...with these sensors, but further optimization is necessary for these to be as effective at the single cell...perform poorly in two-photon conditions, which are necessary conditions for penetrating deeper tissues depths...

...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com... of a learning curve it will be to get to the necessary level  of proficiency.  What kind of content are...

...showed that the selective interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short ...Some chemical labeling strategies such as the bisarsenical dye (Figure 1a) and FAP systems (Figure 1e) ...

...theoretical maximum value, physical titers don’t necessarily give any indication of the infectious titer. ...and convenient. However, these methods are not necessarily precise or accurate for quantifying AAV because...

..., though – someone accepting your CV doesn’t necessarily mean that you are a future employee of the company...might even be a colleague. A mentor does not necessarily need to be someone a lot older than you are; ...

...an expression clone with all of the components necessary for gene expression.     The LR Reaction ...all kind of organisms from mammals to E. coli. Glossary of Gateway cloning vectors Vector type  Vector...

...organization left open the question of how much data was necessary and reasonable. PLOS has since clarified their..., itself, has not finalized its definition of necessary data requirements and likely will not do so (calling...

...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells ... it. Here, we'll cover the promoter, which is necessary for getting the plasmid express our gene of interest...

...plant-specific changes to CRISPR components are necessary to enable genome editing in plant cells.   This...modifications to commonly used CRISPR plasmids are necessary to use the CRISPR system in plant cells. Like ...

...brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly monomeric EGFP variant...Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot ...

... as the brain, robust single-cell labeling is necessary to distinguish individual cells. The Brainbow-... populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes and...

... or obstruct binding sites or motifs that are necessary for post-translational modifications. Despite ...for which the N- and C-terminus of the POI are necessary for its native properties. In these cases, an ...

...Systems Together You may be wondering, “why is it necessary to have all 3 of the different binary systems ...similarly?” The simple answer is that sometimes it is necessary or desirable to control or look at multiple genes...

...prevent cutting at mismatched guide sites, but not necessarily binding to these sites. Thus, these enzyme swaps...perform whole genome sequencing. While this may be unnecessary for many applications, it is the only comprehensive...

...knockdown or overexpression experiment, generate the necessary constructs, and complete screening in just 3 days...This toolkit reduces the expense and workload necessary to produce vectors for genome editing in worms...the initiation of the T. gondii infection, and necessary for the asexual cycle of malaria parasites. Find...

...packaging plasmid containing all the structural genes necessary for the production of a viral particle (Gag, Pol...encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral particle...

...missing? For example, are additional controls necessary? As you build your story, are additional data ...important to have a plan to get to mechanism. Do the necessary tools exist to tackle the problem and if so, do...

...represented below are those of the writers and do not necessarily represent the views of Addgene. Boston, MA, ...breast cancer and infertility or to argue against unnecessary clinic regulations, evidence-based research is...

...sections and 3D cultures These samples do not necessarily require optical sectioning, but open the door...by pixel are unable to provide the frame-rates necessary for imaging tissues of this size. For example,...

...conditions were generated in your lab are necessary for use of a plasmid you are depositing can ...the submitted material under the terms deemed necessary by my institution or other third party rights ...