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We narrowed to 73 results for: dna plasmids

Showing: 1 - 20 of 73 results

  1. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids in our...next generation sequencing Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ...performed on my cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence has been validated...different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA is designed...cloning grade DNA preps of this plasmid available, but helps to inform us of which plasmids would be most...cloning grade DNA for over 200 of our most popular plasmids! DNA... DNA Service: Cloning Grade DNA Service Details...

  2. Open Enzyme Collection

    Type
    Collection
    ...Synthetic Biology Plasmids BIOFAB Plasmid Set Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Blog: Golden...assembly. Plasmids Explore the collection and order individual plasmids via the links below: DNA Ligases... ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514 pOpen-9N7lig 9 Degrees North DNA Ligase...pOpen-Pfulig Pfu DNA Ligase 165535 pOpen-THEAQlig Taq DNA Ligase 165554 pOpen-T4gene30 T4-DNA Ligase 165564...pOpen-ECOligA E. coli DNA Ligase 165569 pOpen-Tthlig Tth DNA Ligase 165574 pOpen-HSlig3 DNA Ligase III Return... to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase...Tli DNA polymerase (exo-) 165524 pOpen-T4gene43 T4 DNA Polymerase 165532 pOpen-ECOpolA E. coli DNA Polymerase...

  3. CRISPR Guide

    Type
    Collection
    ...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...Browse Plasmids: RNA Targeting , RNA Editing Degrading DNA with Cas3 Rather than initiating DNA cleavage...-stranded DNA plasmid. Small ssDNA oligos often work well for small edits like a single-point mutation...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...

  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter... purify a region of genomic DNA and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added to ...

  5. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter... purify a region of genomic DNA and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG...variety of Cas-containing plasmids. Do you have suggestions for other plasmids that should be added to ...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent... cleave all four DNA strands at once. They can mediate the integration of a large DNA payload containing...same DNA strand in opposite directions, recombination results in an inversion and the region of DNA between... circular piece of DNA (and is not maintained). C) If the sites are on separate DNA molecules (in trans...recombination of the DNA between target sites. Codon optimization: Modifying the DNA sequence of the recombinase... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter... purify a region of genomic DNA and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...cerevisiae LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other plasmids that should be added to ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...family. After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can...CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single plasmid, though...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert ...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...posts , or find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models...editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature . 533(7603):420-4....Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive...specifically, specialized Cas proteins snip foreign DNA into small fragments approximately 20 bp in length...length and paste them into contiguous stretches of DNA known as CRISPR arrays. Separate Cas proteins then...motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ...protospacer sequences that have homology to foreign DNA. Protospacers are separated by short palindromic ...

  10. TALEN Guide

    Type
    Collection
    ... “multiple DNA fragments in an ordered fashion in a single reaction.” Using these plasmids, which are ...Guide to TALEN technology and using TALEN plasmids TALENs...Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in...Imagine being able to manipulate a specific region of DNA in the genome of your favorite species almost as ...biological disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of ...enzymes and other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified...expect that assembling relatively small, repeat-laden DNA regions into a single construct would be technically...

  11. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA....precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments of DNA at specific...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases...guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless", as...homology sequences are integrated along with the donor DNA. The two main classes of CASTs are type I and type...

  12. CRISPR Plasmids and Resources

    Type
    Collection
    ...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

  13. CRISPR Plasmids - Plants

    Type
    Collection
    ...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...expression plasmids that have been designed for use in plants. CRISPR...

  14. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...vector is produced from a cargo plasmid in Addgene’s collection. These plasmids are created by your colleagues...Virus-associated DNA Each viral vector service request will also include virus-associated DNA, which is a ...a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...widely cited in publications. All pricing includes DNA amplification, viral vector production, purification...inclusion is what gives you permission to use the plasmid....

  15. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids that create double strand breaks in DNA....genomic changes, researchers use DNA repair templates with homology to the DNA flanking the DSB and a specific...Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert...elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  16. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance... Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ... repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should ...module plasmids and improve the success rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2... TALE plasmids. Genome...

  18. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break....Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors ..., creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been ...

  19. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed ...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...SARS-CoV-2 Plasmids for Many Expression Systems Collection: COVID-19 and Coronavirus Plasmids & Resources...Browse Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems. Depositor...Ginkgo Bioworks COVID-19 Ginkgo Bioworks COVID-19 Plasmid Collection (Link opens in a new window) Researchers...therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many ...Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities, in collaboration...

  20. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule can then...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...
Showing: 1 - 20 of 73 results