We narrowed to 56 results for: rest

...adenoviral plasmid. An adenoviral plasmid containing the rest of the adenoviral genome necessary for viral production... transfer plasmid) containing the transgene of interest, the ITRs, and homology regions for recombination...vector production. Want a plasmid with your gene of interest? Search our database for shuttle plasmids with...

...OZ551 and OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa REST corepressor 2 OZ553 and OZ554 OPEN OPEN gCTCACCATCtgttggaTGCGATGGAa...

...Adult Dermal Fibroblasts Neurons Lentiviral Human REST suppression mediates neural conversion of adult ...

...Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest/RCOR1 [K72/8R] Co-Rest/RCOR1 Human Mouse IgG2a 140068 Anti-Kv1.6...GluN2C/NR2C Rat Mouse IgG2a 222145 Co-Rest/RCOR1 [K72/2R] Co-Rest/RCOR1 Human Mouse IgG2a 222146 Calbindin...

...protein of interest. Signal strength is determined by how many antibodies bind the protein of interest, while... antibody of interest to a second, independent antibody for the same protein of interest, with no overlapping...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called... of the animal’s immune system. The antigen of interest is introduced to the animal, which then mounts...primary antibody which is bound to your protein of interest. Secondary antibodies can be isotype-specific ...recognize an available epitope on your protein of interest. Polyclonal antibodies are typically collected...Western blots should be run with your protein of interest and at least one ubiquitously expressed protein...

...-CDS issues. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI Restriction Sites to Avoid: NotI For...Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI, PstI Restriction Sites to Avoid: PvuII, ... use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix...backbone can be repeated to ligate your gene of interest after the promoter, and so on. The following table... reverse complimented for primer design. The restriction enzymes used for assembly are the prohibited ... Employs high-efficiency enzymes. Prohibited Restriction Sites: EcoRI, BglII, BamHI, XhoI For more info...giving in-frame scar encoding Thr-Ala. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI For more info,...

...promoter upstream of your gene of interest. If your gene of interest should be generally active and only... achieve >10,000-fold induction of the gene of interest. Tight TRE promoter or P Tight : reduced background...Considerations Expression System If your gene of interest should only be expressed occasionally, use Tet-On...tet-responsive promoter upstream of your gene of interest. Many plasmids use "TetO" or "Tet-O" in their ... transactivator and the tet-controlled gene of interest from two separate vectors. Stable cell lines or... transactivator and the tet-controlled gene of interest from one plasmid. A single vector approach may... cloning to control expression of your gene of interest. ID Plasmid Description Transactivator Promoter...

...control and optimize expression of your gene of interest. Inducible: The recombinase may be fused to a ...inversion, deletion, or translocation of a gene of interest. Using Cre or Flp as examples, here are a few ...or "LSL" cassette) placed upstream of a gene of interest will prevent gene expression in the absence of... Switch: These constructs contain two genes of interest, Genes A and B. When Cre is absent, only Gene ...and type of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool... integration of a plasmid containing a gene of interest and another FRT site. Flp recombinase recognizes...resulting in the stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing...

...Express gene of interest from the CMV promoter pCDH-EF1 72266 Express gene of interest from the EF-1 promoter... Express gene of interest from the CB promoter pCDH-PGK 72268 xpress gene of interest from the PGK (phosphoglycerate...Express gene of interest from the CB promoter pCDH-EF1s 72484 Express gene of interest from a truncated...respectively, and empty vectors to express your gene of interest under the control of a variety of promoters. Professor...kinase I) promoter pCDH-CMV4 72284 Express gene of interest from the CMV promoter. WPRE has been removed to...

...use bacteria as model systems to answer many interesting biological questions. Escherichia coli is the...Visualization To determine the location of your protein of interest within the cell, you will need to tag it with ...protein (FP) tags so you can express your protein of interest fused to FPs and monitor their localization in... spectra overlap. That way if your proteins of interest interact, they will bring both FPs together and... only exciting the donor. In BiFC, proteins of interest are fused to each part of a split FP. When the...split FPs that can be fused to your proteins of interest in bacterial FRET and BiFC studies. ID Plasmid...Two-Hybrid (B2H) System allows you to fuse proteins of interest to transcriptional regulatory elements that will...

...and are designed for a user to insert a gene of interest. They may contain tags or fusion proteins, a multiple...allow you to test the function of your gene of interest in a controlled environment under various conditions... C-terminal 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal Myc...an N-terminal GST tag fusion with your gene of interest pFastbac1 Nterminal GST - Baculovirus expression...cell lines in which the expression cassette of interest is incorporated into the host DNA. For mammalian...you can clone your regulatory element or gene of interest to create a luciferase reporter Promoter Measure...HA or myc-BioID2-MCS - To fuse your protein of interest to the N-terminus or C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3...

...you can clone your regulatory element or gene of interest into to create a luciferase reporter. Unless otherwise...Retroviral expression of firefly luciferase and gene of interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral...Lentiviral vector for insertion of your gene of interest upstream of IRES-firefly luciferase. Bryan Welm ...expression of firefly luciferase and dTomato. A gene of interest can also be inserted into the plasmid. Malcolm...construct that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return ...

... code and incorporate a ncAA into a protein of interest, four key modifications to the standard translation..., and opal codons (UGA). The amber codon is the rarest codon in Escherichia coli , which makes it the ...efficiency. A target plasmid containing the gene of interest with the modified codon that is recognized by ...non-canonical amino acids (ncAA) in a protein of interest. Applications By making small changes in selected...pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest 209738 pAB228i16 G1 PylRS Methanogenic archaeon...sites changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306...

...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

...incorporation of phosphoserine into your gene of interest....choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030 origins or a low copy p15a...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated...identify candidate human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented...

... The following resources may be of interest for groups interesting in implementing intersectional experimental...strategies to define cellular sub-populations of interest, including dual-transgenic recombinase-expressing...testing of novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online...

...various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast.... overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization of some of...tagging vectors to create fusions of proteins of interest with yeast codon-optimized fluorescent proteins...

...manipulation to model a specific disease or process of interest. Do you want to: Generate complete and permanent...Intereference : Target the promoter region of the gene of interest, where the Cas9 protein can block transcription... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ...Some prime and base edits Can be paired with restriction enzymes for further customization Usually paired...

...advantage of this to stably integrate transgenes of interest into a variety of cell types. Wild-type gamma-...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...Plasmids Want a retroviral plasmid with your gene of interest? Search our database for retroviral transfer plasmids...

...determining subcellular localization of a protein of interest. One of the most common is to use fluorescence...localization pattern, you should fuse your protein of interest with a fluorescent protein that has a different...localization or possible function of a protein of interest. Browse or search the table below to find markers...full catalog for a marker for your structure of interest. Additional Resources Allen Institute for Cell...