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We narrowed to 123 results for: tra

Showing: 1 - 20 of 123 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...element; tTA: tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation...reverse tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation...in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was created... Plasmid Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression...Stem Cell Research Plasmids Collection Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids...present, TetR binds to tetracycline and releases tet O, enabling transcription (Figure 1). While TetR ...shown. Tetracycline Off (Tet-Off) The first major advance was the Tet-Off system. A tetracycline-controlled...

  2. Synthetic Biology - Strains

    Type
    Collection
    ...Signaling Strains Browse Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial...Strains for use in Synthetic Biology. Plasmid... Plasmid Collections Synthetic Biology Bacterial Strains Synthetic...Synthetic Biology: Bacterial Strains SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio... Bacterial Strains Search the table by keyword or sort by the table headings. Click on the publication...

  3. AAV for Neuronal Tracing

    Type
    Collection
    ...Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal Tracing These AAV encode... EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons...encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies virus. Viral...encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus...virus to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-...Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases (CASTs) combine...tables below for CRISPR transposase plasmids. To learn more about CRISPR transposases and other CRISPR topics...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...combine the large-scale capacity of transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems...lacks nuclease activity, instead recruiting the transposase (comprised of multiple components) to target ...locations in the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs...CASTs is not considered "scarless", as the transposon homology sequences are integrated along with the donor...

  5. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids that create double strand breaks in DNA.... functional Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

  6. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild-type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...TCRAV7S1, TRAV11 TRAV1-2 T cell receptor alpha variable 1-2 TCRAV1S2, TCRAV7S2, TRAV12 TRAV10 T cell receptor...TCRAV2S1, TRAV122 TRAV12-3 T cell receptor alpha variable 12-3 TCRAV12S3, TCRAV2S2, TRAV123 TRAV13-1 T cell...TCRAV8S1, TRAV131 TRAV13-2 T cell receptor alpha variable 13-2 TCRAV13S2, TCRAV8S2, TRAV132 TRAV14DV4 T cell...TCRAV8S1, TRAV81 TRAV8-2 T cell receptor alpha variable 8-2 TCRAV1S5, TCRAV8S2, TRAV82 TRAV8-3 T cell ...TCRAV8S3, TRAV83 TRAV8-4 T cell receptor alpha variable 8-4 TCRAV1S2, TCRAV8S4, TRAV84 TRAV8-6 T cell ...TCRAV8S6, TRAV86 TRAV8-7 T cell receptor alpha variable 8-7 (non-functional) TCRAV8S7, TRAV87 TRAV9-1 T cell...non-functional) - TRAJ10 T cell receptor alpha joining 10 - TRAJ11 T cell receptor alpha joining 11 - TRAJ12 T cell...

  8. CRISPR Guide

    Type
    Collection
    ...may be a single-stranded oligonucleotide, double-stranded linear DNA, or a double-stranded DNA (dsDNA) plasmid...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems... HNH, cleave opposite strands of the target DNA. This results in a double-strand break (DSB) within the...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap, ...Tn7-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions...recruits the transposase proteins to the target location in the genome, where the transposase then carries...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP...measurements of citrate concentrations. PLoS One. 2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse...fluorescent indicators for imaging intracellular potassium ion concentration. Commun Biol. 2019 Jan 14;2:18...Yasushi Okada ATP (extracellular) GRAB_ATP1.0/ATP1.0mut biosensor for extracellular ATP (Constitutive ...photostability and reduced ph interference demonstrates augmented transmembrane chloride movement by gerbil prestin...Susan Amara , Susan Ingram Citrate Nanosensors for in vivo measurements of citrate (CitA) Engineering genetically...biosensor for intracellular glucose Quantitative in vivo imaging of neuronal glucose concentrations with a genetically...

  10. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...serotype is demonstrated to have enhanced transduction of the retina following intravitreal injection in...also displays enhanced transduction of the trabecular meshwork following intracameral injection in mice, ...enhanced transduction of the retina following subretinal administration and enhanced transduction and spread...) serotype shows enhanced transduction of the retina following intravitreal injection in mice and macaque...macaque. This serotype also demonstrates enhanced transduction of cells in culture for ex-vivo conditioning...Anterior Chamber Results in Efficient Transduction of Trabecular Meshwork in Mouse and Rat. PLoS One ....High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for ...

  11. Plan Your Experiment

    Type
    Collection
    ... interfere with transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa...fused to transcriptional activators that need to be within a given range of the transcription start site... perform by chemical transfection or electroporation. Expression can be transient, or you can generate...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...into cells. These methods are more transient than plasmid transfection or viral vectors, reducing the risk...your target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead...is helpful in cells that are difficult to transfect, transduce, or may not express common promoters. Browse...

  12. Adenovirus Plasmids

    Type
    Collection
    ...adenovirus terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398...containing transgene under CMV promoter Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses...Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene under CMV ...genome. Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune...viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production...production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal...Collection of adenovirus shuttle vectors and strains Viral...

  13. Lentivirus Plasmids

    Type
    Collection
    ...divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked...expression, multicistronic design enables tracking of viral transduction. See plasmid 46948 for additional variant...knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey et al. (2011) for additional...20342 FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch 131626 pHAGE-mt-mKeima...envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging...types of viruses used in research. Lentivirus can transduce both dividing and non-dividing cells without a...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...so cells must be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia...emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase reaction...can be used to study processes beyond transcription and translation. Splicing reporters from Younis et al...insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. Its robust...Regulatory elements such as promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can..., and different luciferases require different substrates and detection wavelengths. Firefly luciferase...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Adenoviral High transduction efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle...production AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-...miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells with the Tet-On or...expression Find yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian...Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression page Bacteria Lac...gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for bacterial...

  16. Open Enzyme Collection

    Type
    Collection
    ...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...32 single-stranded DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes You may also ...

  17. Bacterial Expression Systems

    Type
    Collection
    ...extensive selection of plasmids using tetracycline-regulated transcriptional activation or repression. The BIOFAB...generally necessary to purify it. Specific bacterial strains such as E. coli BL21(DE3) are used as biofactories...proteins in high yields. Proteins can then be extracted from the culture and purified through affinity...bacterial protein contamination and facilitates extraction. Browse our most popular plasmids for protein...MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...

  18. AAV Molecular Tools

    Type
    Collection
    ...Overexpression Tracers Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...service encoding tet-off transactivators and tools for affinity purification (TRAP). Viral...Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology...silencing neurons 8 Ivo Spiegel Anterograde Transsynaptic Tracers These AAV encode tools that can be used ...pAAV-ATLASsnCre Syn-driven, constitutive Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic...CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Viviana Gradinaru 99120 pAAV-ihSyn1...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...special trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps... double stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...have long utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...

  20. Genetic Code Expansion

    Type
    Collection
    ...protein translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery... Mehl 85496 pDule-Tet2.0 Tetrazine2.0 tRNA synthetase M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan Mehl...Mehl 85497 pDule2-Tet2.0 Tetrazine2.0 tRNA synthetas M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan Mehl...barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2...Bacterial TAG Wei Niu Browse Strains The table below highlights bacteria strains that have been modified to...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...Rinehart Lab reagents tRNA/Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for ...
Showing: 1 - 20 of 123 results