...tetR will bind to tetracycline and not to the TRE, permitting transcription. Tetracycline-dependent promoters...To use tetracycline as a regulator of gene expression, a tetracycline-controlled transactivator (tTA) ...Overview of tetracycline regulated inducible transcriptional activation or repression Plasmid...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...Collections Background Tetracycline Off Tetracycline On Experimental Tips Tet Plasmids Background To advance the...induction with efficient tetracycline uptake. Search our collection for all tetracycline plasmids. Tet System...19 nucleotide tetracycline operator (tetO) sequence, and is recognized by the tetracycline repressor (tetR...

...Signaling Strains Browse Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial...Strains for use in Synthetic Biology. Plasmid... Plasmid Collections Synthetic Biology Bacterial Strains Synthetic...Synthetic Biology: Bacterial Strains SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio... Bacterial Strains Search the table by keyword or sort by the table headings. Click on the publication...

...Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal Tracing These AAV encode... EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons...encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies virus. Viral...encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus...virus to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-...Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell...

...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting...lacks nuclease activity, instead recruiting the transposase (comprised of multiple components) to target ...locations in the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs...CASTs is not considered "scarless", as the transposon homology sequences are integrated along with the donor...

...CRISPR plasmids that create double strand breaks in DNA....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants...

...TCRAV7S1, TRAV11 TRAV1-2 T cell receptor alpha variable 1-2 TCRAV1S2, TCRAV7S2, TRAV12 TRAV10 T cell receptor...TCRAV2S1, TRAV122 TRAV12-3 T cell receptor alpha variable 12-3 TCRAV12S3, TCRAV2S2, TRAV123 TRAV13-1 T cell...TCRAV8S1, TRAV131 TRAV13-2 T cell receptor alpha variable 13-2 TCRAV13S2, TCRAV8S2, TRAV132 TRAV14DV4 T cell...TCRAV8S1, TRAV81 TRAV8-2 T cell receptor alpha variable 8-2 TCRAV1S5, TCRAV8S2, TRAV82 TRAV8-3 T cell ...TCRAV8S3, TRAV83 TRAV8-4 T cell receptor alpha variable 8-4 TCRAV1S2, TCRAV8S4, TRAV84 TRAV8-6 T cell ...TCRAV8S6, TRAV86 TRAV8-7 T cell receptor alpha variable 8-7 (non-functional) TCRAV8S7, TRAV87 TRAV9-1 T cell...non-functional) - TRAJ10 T cell receptor alpha joining 10 - TRAJ11 T cell receptor alpha joining 11 - TRAJ12 T cell...

...may be a single-stranded oligonucleotide, double-stranded linear DNA, or a double-stranded DNA plasmid. ...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems... HNH, cleave opposite strands of the target DNA. This results in a double-strand break (DSB) within the...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap, ...Tn7-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions...recruits the transposase proteins to the target location in the genome, where the transposase then carries...

...measurements of citrate concentrations. PLoS One. 2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse...fluorescent indicators for imaging intracellular potassium ion concentration. Commun Biol. 2019 Jan 14;2:18...During Nutrient Transitions. ACS Sens. 2020 Mar 27;5(3):814-822. Bas Teusink ATP (extracellular) ecAT3.10 extracellular... extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP with a genetically-encoded...Yasushi Okada ATP (extracellular) GRAB_ATP1.0/ATP1.0mut biosensor for extracellular ATP (Constitutive ...photostability and reduced ph interference demonstrates augmented transmembrane chloride movement by gerbil prestin...Susan Amara , Susan Ingram Citrate Nanosensors for in vivo measurements of citrate (CitA) Engineering genetically...

...another transfection method such as cationic liposome-based transfection. Optimize transfection conditions... (Crick) strand while sgRNA-B’s protospacer sequence and PAM fall on the top (Watson) strand ( Figure ..., which is used for MEL cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully...using preferred culture conditions and transfection strategies for each cell type. While MEL cells are... μl of DNA extraction solution. NOTE: Generally ~100,000 cells are used for DNA extraction, although a...for 6 min, 98 °C for 2 min to extract gDNA. Measure the DNA concentration. NOTE: While steps 6.1 and 6.2...Aspirate media. Add 50 μl DNA extraction solution per well, resuspend and transfer to 96-well PCR plate. Continue...

...serotype is demonstrated to have enhanced transduction of the retina following intravitreal injection in...also displays enhanced transduction of the trabecular meshwork following intracameral injection in mice, ...enhanced transduction of the retina following subretinal administration and enhanced transduction and spread...) serotype shows enhanced transduction of the retina following intravitreal injection in mice and macaque...macaque. This serotype also demonstrates enhanced transduction of cells in culture for ex-vivo conditioning...Anterior Chamber Results in Efficient Transduction of Trabecular Meshwork in Mouse and Rat. PLoS One ....High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for ...

...mutation, the best HR template for transfection is probably a single stranded DNA (ssDNA) oligo which usually...discussing conversion tract length in drosophila. This 1998 study suggests mammalian cells' tract lengths seem ...positively transfected. Alternatively, you can use a selection marker to select transfected cells (for...recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from cells. ...50ul PCR reaction. The gDNA extracted in this way is usually more concentrated than using other kits like...nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does. There are ... The human U6 promoter prefers a 'G' at the transcription start site to have high expression, so adding...

...Plasmids 101: E. coli strains for protein expression Plasmids 101: Common Lab E. coli Strains Why Bacteria? E...pdCas9-bacteria 44249 pTetO Anhydrotetracycline Stanley Qi Anhydrotetracycline inducible expression of catalytically...light. pCph8 50552 pLtetO-1 Anhydrotetracycline Jeffrey Tabor Anhydrotetracycline inducible expression of ...testing transcription factor binding to reporter plasmid pH3U3-zif268 (omega) . Transcription factors ...Keith Joung Transcription factor testing: Kit contains five plasmids and one bacterial strain for performing... answer many interesting questions. Does my transcription factor bind to a particular sequence? Do these...pwtCas9-bacteria 44250 CRISPR Stanley Qi Anhydrotetracycline inducible expression of wild-type Cas9 from...

...adenovirus terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398...containing transgene under CMV promoter Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses...Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene under CMV ...genome. Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune...viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production...production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal...Collection of adenovirus shuttle vectors and strains Viral...

...expression, multicistronic design enables tracking of viral transduction. Also see plasmid 46948 . Elledge 44011...knockdown, multicistronic design enables tracking of viral transduction. See article for more similar plasmids...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...types of viruses used in research. Lentivirus can transduce both dividing and non-dividing cells without a...stably into the host genome, enabling long term transgene expression. There are some safety considerations...encodes the viral genome (typically called the transfer plasmid ). This table lists common lentiviral ...

...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...32 single-stranded DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding...Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes (Link opens in...

...Adenoviral High transduction efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle...production AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-...miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells with the Tet-On or...expression Find yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian...Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression page Bacteria Lac...gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for bacterial...

...protein translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery... Mehl 85496 pDule-Tet2.0 Tetrazine2.0 tRNA synthetase M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan Mehl...Mehl 85497 pDule2-Tet2.0 Tetrazine2.0 tRNA synthetas M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan Mehl...barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2... TAG Edward Lemke Browse Strains The table below highlights bacteria strains that have been modified to...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...Rinehart Lab reagents tRNA/Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for ...

...special trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps... double stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...have long utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...

...so cells must be lysed or the substrate must be delivered intracellularly in order to measure luminescence...Bioluminescence Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing...including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters...collection of luciferase plasmids for assays of transcriptional regulation and bioluminescent reporters. Plasmid...insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. The robust... protein half-lives, making them useful as transcriptional reporters. However, they are not secreted, ...regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these...