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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 88 results for: IRE

Showing: 11 - 20 of 88 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    .... pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen... expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. ... a Biosafety Level 3 (BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study of the live...Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST...Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin resistance. Other Resources...

  2. Retrograde AAV viral preps

    Type
    Collection
    ...expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression...expression Recombinases Wilson 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression...CamKII Bidirectional Optogenetics Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn Bidirectional, Cre-dependent...Dopamine Optogenetics Activators Inhibitors Bidirectional DREADDs Activators Inhibitors Other Molecular...

  3. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene of interest from...

  4. Ras Pathway

    Type
    Collection
    ...receptor INSRR Insulin receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate JUN Jun proto-oncogene...

  5. Control AAV Preps

    Type
    Collection
    ...Constitutive 1 Deisseroth 105921 pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Patrick 114469...

  6. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 5 Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST CAG eGtACR1 (soma-targeted) mRuby3 Cre...variants Jaws WiChR stGtACR iC++ OPN3 PdCO PPO Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx EF1a/nEF...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ... or Off) None Tight TRE Markus Ralser 64238 pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression...transduction (transient or stable) may depend on the desired sensitivity, level of induction, or amount of background... TRE, miniCMV Maria Lung 16542 pBI-MCS-EGFP Bidirectional promoter (Pbi) for Tet-responsive expression...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian U6 yes, cut S. pyogenes Kuehn pMCS-rybozyme-IRES-CAS9 64668 Mammalian yes, cut S. pyogenes W Fujii...Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you have to express both a Cas protein and... plasmids that do not co-express a Cas protein require a separate plasmid that does so; however, these...these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...Apramycin Zhao pRB1017 59936 Worm BsaI none S. pyogenes Fire pGL3-U6-sgRNA-PGK-Puro 51133 Mammalian none S. pyogenes...expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369 ). Joung ...

  9. CRISPR Guide

    Type
    Collection
    ...combination of wild type alleles, NHEJ-repaired alleles, and the desired HDR-edited allele. Therefore, it is...presence of the desired edit experimentally and to isolate clones containing the desired edit (see: Validate...) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired edit is incorporated...transcription-activator-like effector nucleases (TALENs) required scientists to design and generate a new nuclease...the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout assay are simple. You’...continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches matters...of the PAM sequence. The resulting DSB is then repaired by one of two general repair pathways: the efficient...

  10. Plan Your Experiment

    Type
    Collection
    ...BioRender.com. Select Your Desired Genetic Manipulation Different genetic manipulations require different CRISPR...percentage of DNA breaks will be repaired by NHEJ instead of incorporating your desired edit through HDR. Base ...methods and often require screening a larger pool of cells to find one with your desired edit. Lastly, you...base edits Can be paired with restriction enzymes for further customization Usually paired with a sequencing...must be experimentally verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, ...or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency compared...Activate Design and Clone Your gRNA Achieving your desired edit comes down to location, location, location...
Showing: 11 - 20 of 88 results