We narrowed to 60 results for: %s
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TypeCollection...effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence designed...
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Bikard Lab - CRISPR Repression Collection
TypeCollection...Cui, L., Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection... zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols ... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...expression empty vector pFA6a vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted...expression pFA6a vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal ...display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac ... Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN kits - Construct...capture and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection... -
The Pleiades Promoter Project
TypeCollection...Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J...selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94. doi: 10.1073/pnas... -
CRISPR Plasmids - C. elegans
TypeCollection...Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM... -
Mammalian RNAi Tools
TypeCollection... Marmari, V., Ebrahimi, M., Rashno, M. M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms... opens in a new window) . Rao, D. D., Vorhies, J. S., Senzer, N., & Nemunaitis, J. (2009). siRNA vs. shRNA... -
AAV for Neuronal Tracing
TypeCollection... Neuronal Tracing References Wickersham IR, Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal...Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. 2007b. Monosynaptic... -
Plan Your Experiment
TypeCollection...endogenous gene(s) without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting... (CRISPRi) Reduce expression of a particular gene(s) without permanently modifying the genome dCas9-repressor...repressor (such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB ... -
CRISPR Plasmids - Drosophila
TypeCollection...Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM... -
Plasmids for Stem Cell Research
TypeCollection...induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating...single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus ... of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes...fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Parmar Fibroblasts...tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Wernig Fibroblasts ... -
Microbiology Resources
TypeCollection...constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae... -
Fluorescent Protein Guide: Biosensors
TypeCollection...657. Douglas Kim , GENIE Project Calcium GCaMP6f/m/s calcium sensors for imaging neural activity (Constitutive...and mRuby2 for imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent) Cell-specific restoration...Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive... encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Ca2+ indicators based...genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer Zinc Cytosolic...single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic GMP)...Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. Katalin Torok Glutamate... -
Viral Vectors
TypeCollection...'s Biosafety Resource Guide . References Cohen S, Au S, Panté N. How viruses access the nucleus. Biochim... -
Trimmer Lab NeuroMab Collection
TypeCollection...190500 Homer1L/S scFv [L113/130] L113/130 scFv 2a Homer1L/S Mouse Mouse 190501 Homer1L/S scFv [L113/130...Gamma2L/S scFv [N452/73] N452/73 scFv 2T GABA(A)R, Gamma2L/S Human Mouse 190556 GABA(A)R, Gamma2L/S scFv ... Human Mouse IgG2a 177456 Anti-Homer1L/S [L113/130R] Homer1L/S Mouse Mouse IgG2a 177457 Anti-Homer1L [...IgG2a 177553 Anti-GABA(A)R, Gamma2L/S [N452/73R] GABA(A)R, Gamma2L/S Human Mouse IgG2a 177554 Anti-GABA...N476/9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [...Human Mouse IgG2b 206636 Anti-Homer1L/S [L113/130R-2b] Homer1L/S Mouse Mouse IgG2b 206637 Anti-Homer1L... Human Mouse IgG1 206687 Anti-Homer1L/S [L113/130R-1] Homer1L/S Mouse Mouse IgG2a 206688 Anti-Homer1L ... -
Ginkgo Bioworks COVID-19 Collection
TypeCollection...6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5) Mutations Wild type (wt)... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification... -
CRISPR Pooled gRNA Libraries
TypeCollection...Human Mouse Fly Yeast T. gondii E. coli P. aeruginosa S. pneumoniae M. tuberculosis M. smegmatis Green monkey...136986 Knockout Human Doench 3rd 2 40,964 Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition...Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening N/A 1 1,499 MusCK Library 174196... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection... Wu Y, Liang D, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D, Li J. Cell Stem Cell . 2013. PubMed ...colonies using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp . 2007. PubMed . Transformation... Gordanpour A, Nam RK, Sugar L, Bacopulos S, Seth A. J. Vis. Exp . 2012. PubMed . Western blotting...