We narrowed to 53 results for: ARA-2

...SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array... system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro...two MS2 RNA aptamers at the tetraloop and stemloop 2 The MS2-P65-HSF1 activation helper protein Full references...backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites...inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid ( PX600, #61592 ) contains...Epub 2013 Aug 29. Erratum in: Cell. 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9... Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014...

...known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau...Rictor RPTOR independent companion of MTOR, complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return...disease. Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID...mTORC2. Background mTORC1 & mTORC2 The better-characterized mTORC1 responds to various signals, including...

...transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs...2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I (Cas3) Type ... with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA...regulation of transcription in eukaryotes. Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino...Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell . 163(3):759-71. PMID: 26422227...contiguous stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR...have homology to foreign DNA. Protospacers are separated by short palindromic repeat sequences. (1) The...

...pAAV-hsyn-GRAB_Ado1.0med Syn GRAB Ado1.0med none Constitutive 2 Yulong Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE...Huebener , Tobias Rose 83899 pAAV-mDlx-GCaMP6f-Fishell-2 Dlx GCaMP6f none Constitutive 1, 9, rg* Gordon Fishell...pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 CAG GCaMP6f none Cre dependent 1, 2, 5, 9 Douglas Kim , GENIE Project 100836 pAAV.CAG....pAAV.CAG.GCaMP6f.WPRE.SV40 CAG GCaMP6f none Constitutive 1, 2, 9 Douglas Kim , GENIE Project 100837 pAAV.Syn.GCaMP6f.WPRE.SV40...AAV-Camk2-NES-Twitch-GR CamKII Twitch none Constitutive 2 Yiyang Gong Calcium Sensor: jRCaMP1 100846 pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40...pAAV-CAG-NIR-GECO2G CAG NIR-GECO2G none Constitutive 1, 2, 9 Robert Campbell Calcium Sensor: HaloCaMP1a 138327....v857.PDGFR CAG iGluSnFr3 v857 none Cre dependent 2 Kaspar Podgorski 234437 pAAV-syn-iGluSnFR4s-NGR-WPRE...

...degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. ... International Journal of Biomedical Science, 13 (2), 48–57. PubMed (Link opens in a new window) . Moore...Ebrahimi, M., Rashno, M. M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological...

...OPEN OPEN gGTCATCTGCcattcGAGGCGGACa REST corepressor 2 OZ553 and OZ554 OPEN OPEN gCTCACCATCtgttggaTGCGATGGAa...modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511 . Oligomerized pool...activity of three-fold or greater in a well-characterized bacterial two-hybrid reporter system but have...

...gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately. They can knockout, ...Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview of the NHEJ repair mechanism. Multiplex ...systems enable researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single...your experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with...the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and Cas12...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...off-target effects by using a single Cas9 nickase and 2 different gRNAs, which bind in close proximity on ...

...constitutive Expression of the tet-off transactivator (tTA). 2 Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible ...receptor fused to GFP for studying cell ablation. 2 Thomas Jessell , Eiman Azim 207997 pAAV hSyn-fDIO-...voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato. 8 Massimo Scanziani 34910 paavCAG-pre-mGRASP-mCerulean... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

... unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic...such as anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found...secondary immune response Monomer with a valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary... ranging from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include...used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative... can be used in the lab to quantify, identify, separate, and locate proteins of interest. The protein ...use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel. Though relative quantification...

...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different delivery...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that...CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006... Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C....

...AAV6, AAV8, AAV11, or AAV Retrograde serotypes and 2 x 10 12 GC/mL for the AAV2 serotype, as titered by...through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL for the AAV1... you know that we don’t expect to produce our guaranteed titer, and will give you the option to proceed...proceed with your order, or not, without a guaranteed titer. Any titer expectations communicated by email ...email will supersede the general guarantees above. We will have our expert viral vector production team carefully...our viral production does not yield the titer guaranteed here or by email, Addgene will contact you to...carried out with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs...

...optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a ... viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description...

...are largely underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part...Cascade on one single plasmid, though many still separate the individual Cas components. Figure 1: Overview...

...optical imaging down to individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled...pipeline, but individual components can be used separately for additional applications. Park & Sheridan ...equimolar ratios and packaged together in one AAV preparation. The depositing lab recommends keeping these ...

...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then...specific antibody in order to generate high quality preparations. Following production, all antibody preps are...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...staining. For Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside serial...Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or...Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled...

...Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis, and rice. Proof-of-concept performed in zebrafish...

...and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from ...Optimization Kit Using SUMO Protease Elution Protein Characterization Christopher Bahl A validation set of 96 unique...single-domain proteins that are structurally characterized (PDB-deposited), disulfide-free, mesophilic,...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...

...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences... Mandelbaum G, Taranda J, Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi Venkataraju K, Straub C, Wang...payloads in target populations defined by multiple parameters. Depositor...payloads in target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population...combination of genetic and/or anatomical-defined parameters, by placing two orthogonal recombinase (Cre and...Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron 102(3):636-652. PubMed (Link...

... and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein.... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

... proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand pairs bridge...IPI is providing access to high-quality, well-characterized antibodies in an open science model. Learn more...