We narrowed to 101 results for: CAN

...lineage conversion can be faster, bypasses intermediate pluripotent stages, and can occur without cell...available from Addgene that can be used to create iPSCs or another cell type. You can also search the iPSC Neurodegenerative...Pluripotent stem cells, such as embryonic cells, can potentially give rise to any other cell type in the...stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell types, but are typically... cell use and has the major advantage that iPSCs can potentially be derived from patient-specific or disease-specific...the table below. Once iPSCs have been created they can be directly differentiated into specific somatic ...Disease Initiative (iNDI) Collection for plasmids that can be used to create cell lines with endogenously-tagged...

...collection can be used for screening transcription factors for cellular programming or cancer drug resistance...library containing the entire pooled collection, can be purchased at Addgene. Each transcription factor...isoform in pooled screens. This collection and library can be used for screening transcription factors for applications... applications such as cellular programming or cancer drug resistance. Protocols & References A transcription...

...fluorophores at the single-nanometer scale, scientists can use FRET to report on protein binding or conformational...demonstrated for use in FRET experiments. FRET efficiency can be measured using fluorescence microscopy or spectroscopy...fluorophore and emission of a photon) of the donor can be measured using fluorescence lifetime imaging microscopy...for FLIM measurements Note that FRET measurements can be influenced by many factors, such as the organism...Toolkit from the Grefen Lab. FRET pairs and biosensors can also incorporate organic fluorophores via HaloTag...about self-labeling tags on our blog . Luciferase can be paired with a fluorescent protein acceptor for...

...biotin tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved...genomic DNA bound by the gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification...

...microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which...For example, chromosome-specific repetitive loci can be efficiently visualized in living cells using a... close proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that...

.... If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose a.... It has low immunogenicity, is easy to use, and can be safely handled at the lowest biosafety protection... listed on the Function section pages above. You can also do a search of viral preps and filter by the...

..., and, when dysregulated, can enable cancer cell survival. Although cancers are partially defined by their... variants found across cancer types as part of a “pan-cancer” analyses. Cancer Pathways ORFs Kit : Set... Special Collections Cancer Research Cancer Research...Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer Institute... for cancer research, organized by signaling pathway. Special...Addgene has a large variety of resources for use in cancer signaling studies, including mutant, knockdown,...pathway, focusing on the main pathways involved in cancer development. These pathways are important to normal...

... single-strand DNA degradation. This degradation can continue for many kilobases in one or both directions...effectively turning Cas3 into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions... into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms...

...may yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells...Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning grade DNA? At this...and will require separate shipping charges. Where can I find cloning resources or scientific support? Please...

... binds tightly to a specific antigen, scientists can use it to pull out a protein of choice from a mix...the R-mAbs are secreted into the culture media and can be collected for use. Other scientists may choose...small size. These recombinant antibody fragments can be used in experiments such as immunoprecipitation...following table highlights plasmid collections that can be used to create and express plasmid-based antibodies...

...an Order: After you have created an account, you can place orders through our website. Please note that...Recipient Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who ...institution. An authorized signatory is someone who can execute legal documents on behalf of the recipient...recipient institution. Once the MTA has been signed, it can be submitted to Addgene through your user account...collection Protein Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...orders must be placed online through our website. We cannot accept orders by phone, fax, or email. Pricing ...

...remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve functionality...for expression in Escherichia coli . Researchers can use the collection as the basis of manufacturing ...choice and transforming into E. coli bacteria. This can help reduce lab costs and overcome supply chain issues... where enzymes are harder to obtain. The enzymes can also be used as a base to improve functionality or...evolution. More information about the collection can be obtained at the Open Bioeconomy Lab's Open Enzyme...

...only (RUO) terms and do not require an MTA. Pricing can be found on the individual product pages. Orders ...purification, visualization, and functional studies. You can even customize ligands for your specific purpose....epitope tag enabling precise protein detection. It can be paired with LgBiT for bioluminescent assays or...options. HiBiT technology's exceptional dynamic range can detect low-abundance proteins at endogenous levels...

...receptors are popular in neuroscience, where they can be used to interrogate brain function and examine...where the receptor is expressed, these receptors can be used to artificially activate or inhibit specific... either excite or inhibit the target neuron. LMO can be activated either by adding the luciferin substrate...

...epitope tag can be used to isolate a given genomic locus. Visualize CRISPR fluorescence methods can be used...systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR...

...Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've...this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety...reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle...uniquely suited to simplify the cloning process as it can be used in blue–white screening . Adenoviral Vector...

...Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to...plasmids that are currently in our repository. You can obtain the plasmid from us or receive a ready-to-... Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional...

...DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger expression of the downstream...doxycycline (Zhou et al., 2006). Tet-On 3G systems can achieve >10,000-fold induction of the gene of interest...description, meaning they contain a TRE or tet O and can serve as either Tet-Off or Tet-On systems, depending...vectors. Stable cell lines or transgenic animals can be made to achieve greater control over expression...example, only a relatively low level of doxycycline can access the brain so a highly sensitive transactivator...profiles for dox and other tetracycline derivatives can be found in Krueger et al., 2004. Some cell culture...may contain tetracycline or its derivatives which can result in unintended induction. Check the manufacturer...

..., allowing for precise gene editing. These edits can include mutations seen in human patients, protein...scalable. Multiple gRNAs targeting multiple loci can be easily expressed in the same cell or organism....amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important to a... Nickase mutant: cuts only one strand of DNA and can be used with paired gRNAs to lower off-target cleavage...fused to transcriptional activators or repressors can modulate transcription in a reversible manner. Epigenetics...Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions and create heritable epigenetic...labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image genomic loci in live cells. RNA ...

...dsRNA can be delivered as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur...similar mechanism. Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding...therapeutic targets, and delivery strategies for cancer therapy . International Journal of Nanomedicine...