...Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids...assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on a split NanoLuc...more. nLuc/cLuc for split Firefly Luciferase complementation in plants. Bioluminescence Resonance Energy...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...

... mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP AICSDP-24 mEGFP Non-muscle ...AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43...69 mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar...mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP RPB1 RNA...

... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Xanthomonas. The central TAL targeting domain is composed of 33-35 amino acid repeats. These repeats only...high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers, potential...TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...

.... With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months)...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific biotin labeling in zebrafish. pGCS Vector...GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas...

...EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast growth... to find available plasmids for the gene. For components with multiple subunits or isoforms, individual...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...

...Addgene's TALEN Guide. These collections and accompanying documentation allow one to efficiently assemble...Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised of 834 plasmids of pre-assembled trimer and tetramer...TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN and TAL Effector Kit 2.0...i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden Gate TALEN kit, (ii) a lacZ ...

... Guide for more information about: Lentiviral components 2nd versus 3rd generation lentiviral systems ...feature of lentivirus used in research is that the components necessary to produce an infectious viral particle...called packaging and envelope plasmids) encode components of the viral capsid and envelope and are used...transfer plasmids along with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making...

...virus Mammalian constructs Due to the size and complexity of the collection, all available Ginkgo SARS-...to identify antibody escape mutants and for comprehensive mapping. Some of the following plasmids are ... Library of Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor... of variant Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler Starr). External...

...CRISPResso CRISPResso is a robust and easy-to-use computational pipeline that enables accurate quantification...visualization of CRISPR-Cas9 outcomes, as well as comprehensive evaluation of their effect on the coding sequence...sequence (without PAM sequence) can be provided, to compare the predicted cleavage position to the position...sequencing, WGS datasets and allows the direct comparison of individual experiments. PubMed PMID 27404874...

...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Plasmids MORF Pooled Library The MORF library is a comprehensive human transcription factor collection that contains...Pooled Library The MORF pooled library is a comprehensive collection of human transcription factor open...

...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in living cells....

... research in small biotech and pharmaceutical companies. The RESOLUTE project has received funding from...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow...efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...

...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...elegans. WormBase - A consortium of biologists and computer scientists dedicated to providing the research...elegans and related nematodes. WormBook - A comprehensive, open-access collection of original, peer-reviewed...

...juxtaposed to the DNA complementary region. The uniqueness of CRISPRi, as compared to several recently ...uide RNA (sgRNA) designed with a 20-basepair complementary region to any gene of interest. Co-expression...

...Density centrifugation purification Addgene’s comprehensive quality control process: Titering Purity assessment... amplify DNA, produce the prep, and perform comprehensive quality control processes to make sure it is...researchers with troubleshooting and results that complement published data, capturing the insights that often...

... - a public web resource composed by a collection of databases, computational and experimental resources...monocots and dicots - Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency...

...for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as...designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant ...Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting...the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...

...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...

...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...