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Showing: 21 - 40 of 117 results

  1. COVID-19 Resources

    Type
    Collection
    ... virus. It also lists several luciferase and fluorescent reporter plasmids that have been used for measuring...RBD mutants that can be used to identify antibody escape mutants and for comprehensive mapping. Some of ... industry is continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI For more information on these genes...COVID-19 research in the table below: ID Plasmid Description Industry PI Do you have a suggestion for plasmids...Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the lab to ask...

  2. Lentiviral Prep Service

    Type
    Collection
    ... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse our list of...based on plasmids in our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...Constitutively active Cas9 mouse Available plasmids are described below: 61408 : Targeting vector for the mouse ... backbone plasmid for cloning new targets are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA...present in the mouse genome. These plasmids are described here. #60227 - AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR...This plasmid enables Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition...plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition...

  4. CRISPR Guide

    Type
    Collection
    ...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

  5. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9...into a customizable DNA labeler compatible with fluorescence microscopy in living cells. Alternatively, gRNAs...specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic loci. ...live cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging... the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking...

  6. Plasmid Collections

    Type
    Collection
    ...Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics...and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more.... set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways ...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology...

  7. Optogenetics Plasmid Collection

    Type
    Collection
    ...Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical...neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for... "transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the... to tag endogenous loci with epitope tags or fluorescent markers. Future Directions It’s amazing to see...for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product....

  9. Microbiology Resources

    Type
    Collection
    ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

  10. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in... Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids... collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell...plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...

  11. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

  12. Institute for Protein Innovation

    Type
    Collection
    ... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

  13. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...plasmids by electroporation 4 . This protocol is described in detail for murine erythroleukemia (MEL) cells...editing efficiency, but 37 °C is acceptable. Fluorescence Activated Cell Sorting (FACS) of Transfected...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...expression of your gene of interest. ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro... shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target...transactivator for your tetracycline system. ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast... for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression...

  15. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

  16. Distribution to Industry

    Type
    Collection
    ... COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards... multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for ...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...

  17. Control AAV Preps

    Type
    Collection
    ...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more....Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

  18. TALEN Plasmids and Kits

    Type
    Collection
    ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains... Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning... and Eric Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the...

  19. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mammalian expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3...pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression ... with multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael...

  20. Chemogenetics Plasmids

    Type
    Collection
    ...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...opto-chemogenetic actuator. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags Eligible...
Showing: 21 - 40 of 117 results