... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR...Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD...Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440...

...emit a specific wavelength of light after excitation by light of a specific wavelength. While more expensive...monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin repeat proteins... constant region. Minibodies - Minibodies are engineered antibody fragments consisting of two scFvs bound... in a range of colors activated by different wavelengths, allowing for easy differentiation between multiple...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ... break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may need to be optimized...

...light-inducible site-specific iCreV recombinase Hongkui Zeng 140135-PHPeB pAAV-EF1a-iCreV can be used to generate...light-inducible site-specific iCreV recombinase Hongkui Zeng 140136-AAV1 pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136-PHPeB pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137-AAV1 pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137-PHPeB pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the...

...before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc...Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries... Cell Migration Consortium Center for Genome Engineering Impact of Genomic Variation on Function (IGVF...

...reproducibility. Some scientists choose to work with full length recombinant monoclonal antibodies (R-mAbs) expressed... functions Expression of IgG Monoclonals with Engineered Immune Effector Functions. Methods Mol Biol. ...6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to obtain ...hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822. Scheeren...

...Recombinases Engel , Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Zeng 51669 AAV...pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng 50465 pAAV-hSyn-EGFP Syn EGFP Control Roth 50469...phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Zeng 174378 pAAV-syn-Flpo Syn Flpo expression Recombinases...

...be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup . ID ... TALengineering Reagents TALE Repeat Plasmids Individual REAL...

...Luciferases AkaLuc : A modified firefly luciferase engineered to use the red-shifted substrate Akalumine with...bioluminescence imaging in vivo . Luciferases with tunable wavelengths : NanoLuc® with either SNAP‐tag or HaloTag7 tag...luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly...Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase Renilla...

... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...Users are also encouraged to join the Genome Engineering newsgroup here . Links to additional pages describing...Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities...

...metabolomics and genome engineering forms the core of the deorphanizing engine. Genetic and proteomic ...voltage all contribute to assess ligand action/engagement. Protein production is used for assays and to...

...dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic worm lines. The transparent...below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids... nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic model organism C. elegans...

...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 55738...GTPases and their GEFs and GAPs: concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249...

...into a single construct would be technically challenging. Thanks to efforts by the Bogdanove group and...targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D...

...phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive...2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Feng 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent...pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent ...

...oncogene homolog, avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN...receptor (TCR) engages with antigen presenting cells of the innate immune system. Engagement of the TCR by...antigens through the B cell receptor (BCR). The strength and duration of signaling controls the fate of...tolerance or anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen...

...silkworm, and budding and fission yeast. Target Finder (Feng Zhang lab) Identifies gRNA target sequences from... non-model invertebrate species. Forums and FAQs Feng Zhang lab: Tips for CRISPR design, homology directed... cloning . CRISPR Discussion Group (moderated by Feng Zhang lab): Forum/FAQ Drosophila: General Advice...

... TALengineering Reagents TALEN Zebrafish Reagents TALENs for...Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to induce targeted mutations...table below lists pairs of TALENs that have been engineered by the Joung, Peterson, and Yeh labs at MGH to...

... GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent...

...dedicated Cre-lox Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN ... custom TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection... luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...

... Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification Controlled... expression systems to find out more! Genome Engineering Although overexpressing or knocking down a gene...purified protein. Protein purification can be challenging, but once you get it right, you can learn many...