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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 95 results for: Eng

Showing: 21 - 40 of 95 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR...Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD...Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440...

  2. Antibody Guide

    Type
    Collection
    ...emit a specific wavelength of light after excitation by light of a specific wavelength. While more expensive...monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin repeat proteins... constant region. Minibodies - Minibodies are engineered antibody fragments consisting of two scFvs bound... in a range of colors activated by different wavelengths, allowing for easy differentiation between multiple...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ... break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may need to be optimized...

  3. Brain Initiative Collection

    Type
    Collection
    ...light-inducible site-specific iCreV recombinase Hongkui Zeng 140135-PHPeB pAAV-EF1a-iCreV can be used to generate...light-inducible site-specific iCreV recombinase Hongkui Zeng 140136-AAV1 pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136-PHPeB pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137-AAV1 pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137-PHPeB pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the...

  4. Antibody Plasmid Collection

    Type
    Collection
    ...reproducibility. Some scientists choose to work with full length recombinant monoclonal antibodies (R-mAbs) expressed... functions Expression of IgG Monoclonals with Engineered Immune Effector Functions. Methods Mol Biol. ...6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to obtain ...hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822. Scheeren...

  5. Plan Your Experiment

    Type
    Collection
    ...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...them. To increase specificity, consider using an engineered Cas enzyme with higher fidelity. In addition ...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system for CRISPR-Based genome editing...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...fungi and bacteriophages are powerful genome engineering tools used to precisely manipulate DNA and control...Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT...Type PI Description dRMCE Plasmid Kit Genome Engineering Rolf Zeller Use this dual recombinase-mediated...recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional alleles in mouse embryonic stem cells...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...Recombinases Engel , Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Zeng 51669 AAV...pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng 50465 pAAV-hSyn-EGFP Syn EGFP Control Roth 50469...phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Zeng 174378 pAAV-syn-Flpo Syn Flpo expression Recombinases...

  8. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup . ID ... TALengineering Reagents TALE Repeat Plasmids Individual REAL...

  9. Plasmid Collections

    Type
    Collection
    ...before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc...Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries...

  10. Resolute Plasmid Collection

    Type
    Collection
    ...metabolomics and genome engineering forms the core of the deorphanizing engine. Genetic and proteomic ...voltage all contribute to assess ligand action/engagement. Protein production is used for assays and to...

  11. Worm Expression Resources

    Type
    Collection
    ...dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic worm lines. The transparent...below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids... nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic model organism C. elegans...

  12. TALEN Guide

    Type
    Collection
    ...into a single construct would be technically challenging. Thanks to efforts by the Bogdanove group and...targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D...

  13. Arf GTPase Family

    Type
    Collection
    ...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 (ARF1GAP...GTPases and their GEFs and GAPs: concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249...

  14. Control AAV Preps

    Type
    Collection
    ...phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Zeng 58909 pAAV-GFAP104-mCherry GFAP104 mCherry Constitutive...2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Feng 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent...pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent ...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...oncogene homolog, avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN...receptor (TCR) engages with antigen presenting cells of the innate immune system. Engagement of the TCR by...antigens through the B cell receptor (BCR). The strength and duration of signaling controls the fate of...tolerance or anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen...

  16. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ... TALengineering Reagents TALEN Zebrafish Reagents TALENs for...Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to induce targeted mutations...table below lists pairs of TALENs that have been engineered by the Joung, Peterson, and Yeh labs at MGH to...

  17. Optogenetics AAV Preps

    Type
    Collection
    ... GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...dedicated Cre-lox Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN ... custom TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection... luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...

  19. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting J, Tasic B. A suite of ...Gradinaru V, Sunkin SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B. Enhancer viruses for combinatorial cell-subclass-specific...Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng H, Tasic B, Lein ES, Ting JT, Levi BP. Functional...

  20. KLF Research Plasmids

    Type
    Collection
    ...Research KLF Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy...Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are ...
Showing: 21 - 40 of 95 results