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Showing: 21 - 40 of 66 results

  1. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9...into a customizable DNA labeler compatible with fluorescence microscopy in living cells. Alternatively, gRNAs...specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic loci. ...live cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging... the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking...

  2. Recombinases AAV Preps

    Type
    Collection
    ...these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI....AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA...Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA...Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none...none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a ...

  3. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

  4. Plasmid Collections

    Type
    Collection
    ...and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more....CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics... set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways ...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology...

  5. Worm Expression Resources

    Type
    Collection
    ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large ...and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  6. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in... Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids... collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell...plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in...

  7. Lentiviral Prep Service

    Type
    Collection
    ...expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse our list of...based on plasmids in our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance...

  8. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy...repetitive regions. Fluorophores may also be fused to gRNA sequences to produce fluorescence at a target region...

  9. Optogenetics AAV Preps

    Type
    Collection
    ...regulatory element Fluorophore GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength...Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mammalian expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3...pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression ... with multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael...

  11. Cre-lox system

    Type
    Collection
    ...expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization...of interest. Many Cre constructs also contain fluorescent labels that indicate if recombination has occurred...create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking ... Cre Mammalian Hughes 16422 betaRBPIG Double fluorescent, double selectable cre/loxP reporter Mammalian...

  12. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents)...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...second expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...

  15. Retrograde AAV viral preps

    Type
    Collection
    ... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ... to tag endogenous loci with epitope tags or fluorescent markers. Future Directions It’s amazing to see...

  17. Synthetic Biology - Metabolism

    Type
    Collection
    ...Include: Biofuels Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table...

  18. Optogenetics Plasmid Collection

    Type
    Collection
    ...Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical...neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral ...

  20. Synthetic Biology - Overview

    Type
    Collection
    ...Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...
Showing: 21 - 40 of 66 results