We narrowed to 152 results for: Gene

...name to find available plasmids for the gene, or browse the gene list below. For proteins with multiple...top Ras Pathway - Gene List Click on a name to find available plasmids for the gene. For components with... p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small GTPase ...a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation and survival...the cell, however when mutations occur in the Ras gene it can be stuck in a constitutively active form,...multiple subunits or isoforms, individual links to each gene page are provided below. Color is used for clarity...multiple subunits or isoforms, individual links to each gene page are provided below. Symbol Name AKT AKT1 AKT2...

...KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR...CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing mouse...based approach to knockout, repress, and activate gene targets. To accomplish this they have taken advantage...pX458 plasmid to expand its usefulness for the lab's gene regulation and developmental studies. Key plasmid...fate specification, yet differs from deleting both genes . Development. doi: 10.1242/dev.190272. PMID: 32541005...pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) Plasmids...Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual guide cloning...

...developments with CRISPR-Cas9 gene editing have propelled both reverse and forward genetics of these parasites. ... these tools have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission...predicted protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors...content for this page was generated with the help of Scott Lindner . Cut ID Plasmid Gene/Insert Selectable Marker.... Moreover, CRISPR-dCas9 gene regulation has also been established for some of these parasites. Together...plasmids that have been used in these efforts. Addgene's parasite CRISPR plasmid collection also includes... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

...name to find available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits...for the gene. For components with multiple isoforms or subunits, individual links to each gene page are...especially important to tumorigenesis. Inheriting only one functional copy of the p53 gene causes a large reduction...content for this page was generated with the help of . Return to top p53 Pathway - Gene List Click on a name... Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription...Cancer Impact p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations ... subunits, individual links to each gene page are provided below. Color is used for clarity and does not...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...Description Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 ...165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert 165509... Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV...Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N...Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506...NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney...Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease 165560 pOpen-T4 g32 T4 gene 32 single-stranded DNA-...

... their cellular machinery to generate viral vectors for research and gene therapy. Created with BioRender.com...cells. These virusal vectors enable long-term stable gene expression and are permanently integrated into the...Adeno-associated viral (AAV) vectors are commonly used for gene delivery in vivo because of their mild immunogencity...viruses. The viral genome contains only the necessary genes to achieve this goal. When a virus recognizes a ...have been stripped of any unnecessary accessory genes and divided into multi-plasmid systems. These systems...safety and are replication-incompetent. Specific genes have been mutated or deleted to prevent uncontrolled...uncontrolled viral replication, and the required genes are divided in the multi-plasmid system. Many viral plasmids...

...name to find available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits...for the gene. For components with multiple isoforms or subunits, individual links to each gene page are...this page was generated with the help of David Sabatini . Return to top mTOR Pathway - Gene List Click on...Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t ... subunits, individual links to each gene page are provided below. Color is used for clarity and does not...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...catabolic processes including autophagy and lysosome biogenesis. mTORC2 does not respond to nutrients, but it...

... editing multiple genes at once; using dual nickases to generate a knockout or gene edit; or using Cas9...the targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the...eliminate target gene function. It has also been used extensively to screen for novel genes that regulate... activate, or repress genes. Each library typically contains ∼3–6 gRNAs per gene to ensure modification...repress, visualize, and isolate genes. Activation or Repression of Target Genes Targeting dCas9 to transcription...single-gene activation (Figure 9D) Figure 9: Overview of CRISPRi/a. In bacteria, activating gene expression... of effective gene activators when fused with dCas9. Recently, synthetic CRISPR-Cas gene activators have...

...Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter ...Nick ID Plasmid Gene/Insert Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter...other insects. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...and aggressive plan to use gene editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA ...mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR...diagnostic criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett...caused from loss-of-function mutations in the MECP2 gene. Causative mutations for Rett syndrome typically...global transcriptional regulator of thousands of genes and studies have suggested roles in transcriptional...activity dependent de-repression, chromatin remodeling, gene activation, and more. How loss of MECP2 protein ...suggests the major function of MECP2 is to regulate gene expression either locally or globally. The MECP2...

...knocking out genes or introducing specific mutations can reveal important insights into gene function. These...expression of a gene of interest in E. coli . The Marionette Sensor Collection (Addgene #1000000137) allows...of up to twelve genes using small-molecule inducers in the same E. coli strain. Addgene Blog Inducible ...bacterial gene biology or engineering metabolic pathways to synthesize a target metabolite, genetic tools ...visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering...transcriptional regulatory elements that will turn on gene expression upon protein–protein interaction. The...always advantageous to constitutively express your gene of interest at high levels. Perhaps high expression...

... anti-CRISPR genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes employ varied...especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important...system - utilizing various CRISPR-associated (Cas) genes to not only store a record of invading phages but...Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive immune system seems...produces insertions/deletions disrupting a target gene. On the other hand, if a repair template with homology...according to this template, allowing for precise gene editing. These edits can include mutations seen ...given phenotype. Current libraries are available for gene knockout, as well as transcriptional activation ...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...and adenine base editors (ABEs) — can be used to generate single base pair edits without double stranded...editing systems include targeted AID-mediated mutagenesis (TAM) from the Xing Chang lab and CRISPR-X from...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection Chemogenetics plasmids encode genetically engineered...Proteins Guide Optogenetics Plasmids Optogenetics Guide Blog: Chemogenetics Blog: Chemogenetics vs. Optogenetics...biological processes. Addgene's collection contains two main classes of chemogenetic receptors: Designer... a bimodal opto-chemogenetic actuator. For more detailed descriptions of chemogenetic receptors, ligands...your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search ...Additional Resources Chemogenetics Guide Viral Vector Packaging Service In-Stock AAV: Chemogenetics Fluorescent...

...RNA-mediated gene silencing mechanism. It is a useful tool to investigate the roles of specific genes or to ...to knockdown genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian cell...Lentiviral vectors are a preferred method for long-term gene silencing. Once the viral vector is taken up by ...RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods in Molecular Biology, 629 , ...Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral...mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated...

...EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR). ...EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR) and...contain genes with modified codons for non-canonical amino acid incorporation. ID Plasmid Gene/Insert ...Removed endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV gene replaced by tRNA variant that suppress...endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV and tyrU genes replaced by tRNA variant that suppress...endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV and tyrU genes replaced by tRNA variant inactivated... for efficiency. A target plasmid containing the gene of interest with the modified codon that is recognized...

... AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Description Gene/Insert Vector Type Promoter PI Return to top Additional Resources Optogenetics Guide Viral...tables, refer to Addgene's Optogenetics Guide . Interested in more news about optogenetics research? Find...Chemogenetics Guide Blog: Optogenetics Blog: Chemogenetics vs. Optogenetics Content last reviewed: 25 September... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection Microbial Opsin...In-Stock AAV: Optogenetics Fluorescent Biosensors Fluorescent Proteins Guide Chemogenetics Plasmids Chemogenetics...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and...

...protein (FP) tags. Information on genes and associated neurodegenerative diseases was curated from several...but not exhaustive. If you do not see a particular gene of interest, please search our full plasmid collection... Huntington's Disease Parkinson's Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized... T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416 25Q GPD HTT GFP... Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a ...Spinal muscular atrophy Eugene Yeo 156073 TARDBP TARDBP V5-MCP EF1a ALS Eugene Yeo 156883 pD649-HAsp-CSF1R-Fc...Parkinson's, FTD Eugene Yeo 214673 lucMAPT-30U MAPT T5, luciferase CMV Parkinson's, FTD Eugene Yeo 214674 lucMAPT-GenRep...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...methylation Epigenetic modification is typically targeted to a specific promoter or enhancer for your gene of ...CRISPR Epigenetics CRISPR Plasmids: Epigenetics Mammalian Plant Resources To make targeted epigenetic modifications... plasmids designed for epigenetic modification. To learn more about epigenetic modification with CRISPR...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include: P300 — activation... local chromatin state. Figure 1: Overview of epigenetic modification using CRISPR. Created with BioRender.com...