We narrowed to 101 results for: NTS

...Empty backbones are plasmids containing only the elements needed for replication in the host cell and are...choosing what plasmid backbone to use, you have many elements to consider. Here is a guide to a selection of... 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal Myc tag for ...-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101... transient expression is sufficient for some experiments, scientists often want to create stable ...species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection... miRNA and 3' UTR mRNA regulation pIS0 - 3'UTR segments of target genes can be inserted into this firefly...

...The RESOLUTE mission is to develop data and reagents to boost the research focused on Solute carrier ...Union. The RESOLUTE mission is to develop data and reagents to boost the research focused on SLCs. Through...-access ethos’ to the results, techniques, and reagents with the highest-possible quality of research ...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...libraries are useful tools for genetic screening experiments. These libraries target the solute carrier family...new window) . If you have further questions or comments you can also contact them at [email protected]...

... collections and resources are up-to-date with reagents and information you need to use CRISPR in your... for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans...of plasmids that are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries...knockout, activation, inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function Genome Engineering...deletions. Insert CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

...Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses (LULLs)...10 business days in order to proceed with any adjustments to the original order. Turnaround Time Production...10 business days in order to proceed with any adjustments to the original order. Experimental Disclaimer...Disclaimer Due to the novel nature of experiments carried out with our materials, Addgene cannot guarantee the...assists importation by including thorough shipping documents and following import procedure, it is the responsibility...

...pseudovirus, where the structural and functional components of one virus (such as HIV, murine leukemia virus...information. Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein ...D614G change which is present in many circulating variants. The table below lists expression plasmids that...Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID...

...(dark) acceptor, used for FLIM measurements Note that FRET measurements can be influenced by many factors...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes...molecules have been demonstrated for use in FRET experiments. FRET efficiency can be measured using fluorescence...Introduction to FRET and Tips for Using FRET in Your Experiments . Find many FRET-based biosensors in Addgene'...

...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV...Whitehead Libraries of Spike (S) Ectodomain and RBD mutants. SARS-CoV-2 Spike Receptor Binding Domain Deep ...Extraction Without A Kit Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...Link opens in a new window) COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv (Link opens in a new ...differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome...

...generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled up for...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...More details on how to use this plasmid in your experiments can be found below and in the supplemental materials... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

... launched in 2008 to drive the development of treatments and cures for Rett Syndrome and related MECP2...syndrome is a neurodevelopmental disorder that presents in early toddlerhood primarily affecting females...skills, notably loss of acquired purposeful hand movements and regression of speech. In contrast to the sustained...there is wide variability in symptoms even within patients with the same mutation, the location and type ...2007. Correlation between clinical severity in patients with Rett syndrome with a p.R168X or p.T158M MECP2...

...intron-lacZ/NLS Ple144 NTSR1 pEMS1137 EGFP/cre/NLS Ple144 NTSR1 pEMS1394 EGFP/NLS Ple146 NTSR1 pEMS1396 EGFP/NLS.../NLS Ple146 NTSR1 pEMS1617 intron-lacZ/NLS Ple147 NTSR1 pEMS1397 EGFP/NLS Ple148 OLIG1 pEMS1169 EGFP/NLS...

...systems, allowing for the integration of large fragments of DNA at specific locations. The Cas effector...recruiting the transposase (comprised of multiple components) to target locations in the genome defined by...for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs...V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type I, type V-K...

...TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...assembly. Links to additional pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN...

...leaders providing research opportunities to their students and other members of the MCC community. They support...courses, at any type of institution, and at different points in their careers to participate in the community...single protein with many available isoforms and mutants provides a shared experience for the research community...PMID: 35921892 (Link opens in a new window) Acknowledgments The MCC and plasmid construction was funded...

...Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v Sarcomeric thick filaments 124606 SOX2-...87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin filaments 87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic...Troponin I, slow skeletal type Sarcomeric thin filaments 114412 TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres...

...mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity...AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep from Addgene... processes to make sure it is ready for your experiments. Shipping 2–7 days We will email you with tracking...Once you start getting results from your AAV experiments, remember to share your protocols and results...

...specific QC experiments performed varies for each viral lot. To learn which specific QC experiments were performed...ddPCR) using primers and probes targeting the ITR elements and an internal control of known titer (protocol...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

... pipeline for the analysis of genome editing experiments. It enables rapid and intuitive interpretation... for the purpose of designing and evaluating experiments. Developed by the Hyongbum Kim Lab . Green Listed... range of reference genomes, including animals, plants, bacteria, fungi, protists, and viruses. Developed...

...these ORF fragments (which have become exons) determines the logical expression requirements of Cre and...References The development of powerful molecular reagents for biology have propelled our understanding of... for the molecular design and testing of novel INTSRECT plasmids for groups interested in producing their...

...recombinant antibody collection. These affinity reagents are expressed and purified from plasmids in our...research. We are building a collection of affinity reagents that are expressed from plasmids. These antibodies...therefore makes the sequences of these affinity reagents available to scientists. Additionally, we have...

... with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by optimizing...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window...