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We narrowed to 100 results for: OMP;

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Showing: 21 - 40 of 100 results

  1. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...parts can be combined to construct complex networks. This is accomplished through the use of assembly standards...suffix sequences that make it easy to combine components for synthetic biology research. Synthetic...meaning that the suffix will need to be reverse complimented for primer design. The restriction enzymes used...

  2. Antibody Production

    Type
    Collection
    ...containing 1 mM sodium azide. This buffer is not compatible for use in live cells and will interfere with...secondary antibody. The fluorescence pattern is compared to an untransfected control. Immunohistochemistry...secondary antibody. The tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue...HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the protein size...

  3. Viral Vector Guides and Plasmids

    Type
    Collection
    ...immunogenicity. Viral vectors also vary in the composition of their viral genome, expression of the transgene...one or more plasmids containing the necessary components of the viral genome to assemble the viral capsids...carefully engineered for safety and are replication-incompetent. Specific genes have been mutated or deleted ... self-inactivating, meaning that they can only complete one round of transcription of their viral genome...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them, or skip ahead... Systems Subsequent efforts have improved the components of these systems for better efficiency, sensitivity... transfected or transduced cells express both components in the same relative ratio. Different generations...transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter, and...et al., 2016 (Link opens in a new window) for comparison of Tet-On systems in different applications. ...

  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP AICSDP-24 mEGFP Non-muscle ...AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43...69 mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar...mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP RPB1 RNA...

  6. TALEN Guide

    Type
    Collection
    ... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Xanthomonas. The central TAL targeting domain is composed of 33-35 amino acid repeats. These repeats only...high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers, potential...TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...

  7. Zebrafish Plasmid Collection

    Type
    Collection
    .... With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months)...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific biotin labeling in zebrafish. pGCS Vector...GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas...

  8. Ras Pathway

    Type
    Collection
    ...EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast growth... to find available plasmids for the gene. For components with multiple subunits or isoforms, individual...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ...Addgene's TALEN Guide. These collections and accompanying documentation allow one to efficiently assemble...Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised of 834 plasmids of pre-assembled trimer and tetramer...TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN and TAL Effector Kit 2.0...i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden Gate TALEN kit, (ii) a lacZ ...

  10. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Plasmids MORF Pooled Library The MORF library is a comprehensive human transcription factor collection that contains...Pooled Library The MORF pooled library is a comprehensive collection of human transcription factor open...

  11. Mammalian RNAi Tools

    Type
    Collection
    ...The siRNA then binds the RNA-induced silencing complex (RISC). RISC unwinds the double-stranded siRNA ...available. To find plasmids containing other RNAi components, like argonaute, please search our full site ...., Sun, H., Zhang, J., & Feng, L. (2023). A comprehensive review of small interfering RNAs (siRNAs): mechanism...

  12. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...or other reasons For these cases, Addgene will promptly notify you by email within a few business days... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...

  13. AAV for Neuronal Tracing

    Type
    Collection
    ...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...

  14. Institute for Protein Innovation

    Type
    Collection
    ...receptors and ligands (Glypicans and Integrins), components in the synapse (Neurexins and Neuroligins), and...Guidance IPI’s Axon Guidance Antibody Collection comprises recombinant monoclonal antibodies with high specificity...are a family of mechanotransduction receptors, comprised of alpha-beta subunit heterodimers. The IPI collection...

  15. Retrovirus Plasmids

    Type
    Collection
    ...for safe use in the lab. The gamma-retrovirus components necessary to produce infectious viral particles...Guide for more information about gamma-retroviral components, viral production, and viral safety. Gamma-Retroviral...mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV For tagging mitochondria with HA epitopes ...

  16. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency of editing...additional sgRNA PE4 — PE2 plus MLH1 (a protein component of MMR) mutant to inhibit MMR PE5 — PE3 plus MLH1... visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting our CRISPR...

  17. Worm Expression Resources

    Type
    Collection
    ...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...elegans. WormBase - A consortium of biologists and computer scientists dedicated to providing the research...elegans and related nematodes. WormBook - A comprehensive, open-access collection of original, peer-reviewed...

  18. AAV Packaged on Request

    Type
    Collection
    ...Density centrifugation purification Addgene’s comprehensive quality control process: Titering Purity assessment... amplify DNA, produce the prep, and perform comprehensive quality control processes to make sure it is...researchers with troubleshooting and results that complement published data, capturing the insights that often...

  19. p53 Pathway

    Type
    Collection
    ... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate..., Lu X, Soron G, Cooper B, Brayton C, Park SH, Thompson T, Karsenty G, Bradley A, Donehower LA. Nature...

  20. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...
Showing: 21 - 40 of 100 results