We narrowed to 83 results for: REV
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TypeCollection... derived from P1 E. coli bacteriophage and S. cerevisiae , respectively. Other tyrosine recombinases include... the region of DNA between the target sites is reversed. B) If the target sites are on the same DNA strand...cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...Tracing CRISPR Plasmids and Resources Content last reviewed on 31 July 2025. Do you have suggestions for other...
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Antibody Guide
TypeCollection...Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus... in the cell, and sample processing can hide or reveal epitopes on an antigen. Monoclonal antibodies only...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...should be run with: a positive control, a sample previously verified to contain your protein of interest ...interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform downstream... -
Genetic Code Expansion
TypeCollection...pAB228v TrpRS S. cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial Ahmed ...things to consider. If you are working off of a previously established protocol, make sure to match the ..., ncAA concentration, and the cell lines used previously. Remember that the orthogonal pairs of synthetase...209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1, M. mazei Bacterial Ahmed Badran 212122... -
Microbiology Resources
TypeCollection... S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab...color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (... -
Trimmer Lab NeuroMab Collection
TypeCollection... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A... -
Plan Your Experiment
TypeCollection...RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a wider window...the gRNA vector you have chosen, so it is best to review the protocol associated with the specific plasmid...doi.org/10.1038/nbt.3081 PMID: 25357182 Content last reviewed on 11 July 2025.... -
Mammalian RNAi Tools
TypeCollection...Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746–759. PubMed (Link opens in a new window..., Zhang, J., & Feng, L. (2023). A comprehensive review of small interfering RNAs (siRNAs): mechanism, ... -
CRISPR Plasmids and Resources
TypeCollection...modified from a bacterial immune system to a revolutionary tool for genome engineering. CRISPR Topic Page...breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...critical for developing therapeutic strategies to prevent viral spread. As a Biosafety Level 3 (BSL-3) agent... Plasmid Collection Return to top Content last reviewed on 01 October 2025. Do you have suggestions for... -
Recombinases AAV Preps
TypeCollection... PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Hongkui... -
Antibody Production
TypeCollection...Usage” and “Delivery” conditions, so we recommend reviewing the specific antibody webpage for further information...the experiment is performed in parallel with a previous lot of recombinant antibody or the hybridoma-derived... -
Viral Vector Guides and Plasmids
TypeCollection...Specific genes have been mutated or deleted to prevent uncontrolled viral replication, and the required...Topics Page Viral Vectors 101 eBook Content last reviewed: 5 August 2025... -
Retrovirus Plasmids
TypeCollection...viral genome encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral...101 eBook Biosafety Resource Guide Content last reviewed: 23 October 2025 Do you have suggestions for other... -
Promega Plasmid Collection
TypeCollection...competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion protein ...NanoBRET® are Promega trademarks. Content last reviewed on: 15 July 2025... -
COVID-19 Resources
TypeCollection...-CoV-2 main protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs...33277466 (Link opens in a new window) Content last reviewed on 26 September 2025. Do you have suggestions ... -
Neurodegeneration Research Collection
TypeCollection...understood, and there are no effective therapies to prevent, treat, or cure most of these disorders. An integrated...age of 60. There is presently no cure or way to prevent progression of PD. Treatment consists mainly of... -
CRISPR References and Information
TypeCollection...bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish,...available for human and mice PDF, 276 KB Content last reviewed on 9 June 2025.... -
Bacterial Expression Systems
TypeCollection...out genes or introducing specific mutations can reveal important insights into gene function. These genetic... CRISPR-Transposons Return to top Content last reviewed on 17 June 2025. Do you have suggestions for other... -
Plasmids for Stem Cell Research
TypeCollection...Sox2 Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency. Cell. 2015... Lentiviral Human Direct Lineage Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from... -
CRISPR Pooled gRNA Libraries
TypeCollection...a single genomic locus. Before ordering, please review the steps for using pooled CRISPR libraries and...Knockout Human Doench 3rd 2 40,964 Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition...