...interest is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA...reagents are appropriate for a given experiment. Make sure to check whether reagents are available to carry... gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then delivered to...match the target locus, but it is also critical to ensure that the gRNA target sequence does NOT match additional...allele may be different owing to the error-prone nature of NHEJ. In HDR gene editing experiments, most ...protein-based genome editing in vitro and in vivo. Nature Biotechnology . 33 (1), 73–80. PMID: 25357182...

...target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions...expanded to incorporate VCre in order to enable three-feature cell targeting. C) Example of viral targeting of...viruses delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic ...Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window) with general principles...Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PubMed...fear-promoting circuit in the extended amygdala. Nature 537(7618):97-101. PubMed (Link opens in a new window...2016. Midbrain circuits for defensive behaviour. Nature 534(7606):206-12. PubMed (Link opens in a new window...

...AAV BsaI yes, cut S. aureus Zhang pX602 61593 Mammalian/AAV BsaI yes, cut S. aureus Zhang pSaGuide 64710...need plasmid 65776 S. aureus Joung BPK2101 65770 Bacteria BsmBI yes, cut S. aureus Joung Multiplex gRNA...designed to be used with, such as S. pyogenes, S. aureus, N. meningitidis, etc. gRNA scaffolds are specific...Crimson Bauer BPK2660 70709 Mammalian U6 none S. aureus Joung pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 71236...64710 Mammalian BbsI none S. aureus Musunuru BPK2301 65778 Mammalian BsmBI none, need plasmid 65775 N. meningitidis...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...

...curated literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information...2008 to drive the development of treatments and cures for Rett Syndrome and related MECP2 disorders. The...delay between 6 and 18 months of age. A defining feature, with onset typically between 18 to 30 months, ...InterRett - international online database of clinical features and genetic characteristics of Rett syndrome (...Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol . 68, 944–950. PMID: 21154482 Neul...

...are no effective therapies to prevent, treat, or cure most of these disorders. An integrated study of ...people over the age of 60. There is presently no cure or way to prevent progression of PD. Treatment consists...the idiopathic (having no specific known cause) nature of the disease, a small portion of cases can be... receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17. Next-generation GRAB sensors for ... new window) A foundation dedicated to finding a cure for Parkinson's disease through an aggressively ...

... one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production. Bhadra S, et al. bioRxiv...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-...Science Journals Coronavirus Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley Online Library...Kit References ACE2 - Andersen, KG, et al, 2020, Nature Medicine The proximal origin of SARS-CoV-2 . TMPRSS2... Inhibitor . FURIN - Andersen, KG, et al, 2020, Nature Medicine The proximal origin of SARS-CoV-2 . BSG...

... you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate...amino acids within a protein, any alterations in structure or function in the protein can be observed. The...working off of a previously established protocol, make sure to match the growth medium, ncAA concentration, ...codon. Therefore many controls must be used to make sure that these conditions are true. Always express first...protein with and without the ncAA in the media to make sure that the full length protein is only made when the...using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3)...

...MGC129975, UI, UROC UCN2 urocortin 2 SRP, UCN-II, UCNI, UR, URP UCN3 urocortin 3 (stresscopin) MGC119002, SCP...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor A/guanylate ...cyclase A (atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide...NR61, RTR NRG1 neuregulin 1 ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, NDF, SMDF NRG2 neuregulin 2 Don-1, HRG2...HRG2, NTAK NRG3 neuregulin 3 HRG3, pro-NRG3 NRG4 neuregulin 4 DKFZp779N0541, DKFZp779N1944, HRG4 NRP1 neuropilin...response is triggered within the first few minutes of exposure to a pathogen and is the dominant defense strategy...

...with Coomassie blue. Protein band intensities are measured using ImageJ software and a standard curve is ...intensities of both the antibody and impurities are measured and the ratio of the antibody protein content ...antibody or the hybridoma-derived equivalent to ensure that potency and specificity have been retained...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

...endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP ...fluorescently tagged proteins of key cellular structures in human cell lines. Depositor...fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full...

...amplification, and high-quality quality control measures such as titering, purity assessment, sterility... comprehensive quality control processes to make sure it is ready for your experiments. Shipping 2–7 days...globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging...including purification, titration, and quality control measures. We have distributed preps around the globe and...

...This new resource described in (Gassaway et al., Nature Methods 2022) is adapted from the synthetic human...Haimovich AD, Rogulina S, Isaacs FJ, and Rinehart J. Nature Communications . 2015. 6, 8130. PubMed (Link opens... Haar CM, Isaacs FJ, Rinehart J, and Jewett MC. Nature Communications . 2015. 6, 8168. PubMed (Link opens... TBK1 Activation to Promote Mitophagy. Heo JM, Ordureau A, Paulo JA, Rinehart J, Harper JW. Molecular ...

...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction... enables the evaluation of TALEN activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate...systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction sites for...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

...are primarily used for determining protein 3D structure and crystallography.... that aims to determine the three dimensional structures of proteins of medical relevance, and place them...empty backbones/controls): ID Plasmid GenBank Key Features 26092 p15TV-L EF456736 Hexahistidine tag with ...

...Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are up-to-...below by function. Addgene's CRISPR collections feature plasmids for a variety of organisms, including ...fused to an epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type...

...order. Experimental Disclaimer Due to the novel nature of experiments carried out with our materials, ...function of any viral vector. Addgene performs QC measures which verify the identity of the viral vector,...thorough shipping documents and following import procedure, it is the responsibility of the importer to pay...

...biology resources available at Addgene. Browse featured collections or depositing labs....Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

... into mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected...Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies or ScFvs. ...

...These mutations can be inherited or arise through exposure to mutagens like radiation or viruses (e.g., HPV...Thompson T, Karsenty G, Bradley A, Donehower LA. Nature. 2002 Jan 3;415(6867):45-53. PubMed PMID: 11780111...Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly K. Nature. 1997 Jun 27;89(7):1175-84. PubMed PMID: 9215639...

...collection of plasmids suitable for FRET experiments to measure protein interactions or conformation changes, as...experiments. The efficiency of the energy transfer is measured using fluorescence microscopy by exciting the ...calibrate the numerous and disparate methods used to measure FRET. Plasmid FRET Pair C5V Cerulean attached to...