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  1. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

  2. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually ...Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The ...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library...Nov;19(11):1371-1375. doi: 10.1038/s41592-022-01638-5. PubMed (Link opens in a new window) rEcoli XpS and...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

  4. Brain Initiative Collection

    Type
    Collection
    ...Fishell 83896-AAV1 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAV9 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain... 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons Yulong Li 140553...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

  5. Adenovirus Plasmids

    Type
    Collection
    ...modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube...information about: Adenoviral components Adenoviral plasmid construction Frequently asked questions about adenovirus...

  6. Retrovirus Plasmids

    Type
    Collection
    ...retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV... information about: γ-Retroviral packaging systems Frequently asked questions (FAQ) about retrovirus Popular...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...

  7. Distribution to Industry

    Type
    Collection
    ...mediated saturation genome editing of human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library Screening...plasmids that are available to industry. Learn more about the available plasmids and how to order them. Distribution...Information page for more information. To learn more about Addgene’s MTA process please visit our Help Center...

  8. mTOR Pathway

    Type
    Collection
    ... kinase C Protor Also known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis...

  9. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... expressing Rep2 and Cap2 Melina Fan 104964 pAAV2/5 AAV5 AAV packaging plasmid, expressing Rep2 and Cap5...production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral integration...out our Viral Vector Packaging Service and learn about our options for hundreds of in-stock preps that ...

  10. Cre-lox system

    Type
    Collection
    ... Trypanosoma brucei Cross 34564 pMSCVpuro-Cre Cre 5'LTR Retroviral Ge 34565 pMSCVhygro-Cre Cre and HygroR...HygroR coexpression Retroviral Ge 34568 WZLneo-Cre Cre 5'LTR Retroviral Ge 35619 pTAT-CRE Cre fused to HIV-...1027-32. doi: 10.1073/pnas.0610155104. Epub 2007 Jan 5. PubMed . Sauer B, Henderson N. 1988. Site-Specific...Transgenes. Proc Natl Acad Sci. Jul 13;101(28):10380-5. doi:10.1073/pnas.0403954101. Epub 2004 Jul 6. PubMed...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ...that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting efficiency....that adding an additional guanine nucleotide to the 5’ of 21-bp guide can increase targeting efficiency....Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...

  12. Viral Production

    Type
    Collection
    ...gram-negative bacterial endotoxin is ensured to be less than 5 endotoxin units per mL. The endotoxin levels are determined... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...

  13. Open Enzyme Collection

    Type
    Collection
    ...exo-) DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-) 165512 pOpen-THEAQpolA Taq DNA Polymerase 165513...engineering and directed evolution. More information about the collection can be obtained at the Open Bioeconomy...

  14. Lentivirus Plasmids

    Type
    Collection
    ...pLionII 3rd FIV-derived (FELIX) plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene of... Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...systems Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral... or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ...pCL-CTIG The inducible cassette contains tTA at its 5' end and an inducible promoter at its 3' end, which... or shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB On Aebischer...

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ... for targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a.... Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to...

  17. COVID-19 Resources

    Type
    Collection
    ...protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease that cleaves hACE2...lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...

  18. Retrograde AAV viral preps

    Type
    Collection
    ...DREADD Gether 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hDlx Inhibitor DREADD Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell...serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced...Janelia Research Campus viral project team in collaboration with David Schaffer (University of California...

  19. Bacterial Expression Systems

    Type
    Collection
    ...the SacB gene, allowing for negative selection on 5% sucrose. Other empty vectors for protein purification...in vitro ? These, in addition to many questions about bacteria themselves, can be answered using model...overexpressing or knocking down a gene can tell you much about its function and/or alter cellular activity in a...basic biology of a bacterial gene or redirecting metabolic pathways to produce a therapeutic compound, there...once you get it right, you can learn many things about your protein’s function, structure, and interactions...molecules, light, temperature, or even intracellular metabolite levels. Different plasmids and, often times, ...pCph8 50552 pLtetO-1 Anhydrotetracycline Jeffrey Tabor Anhydrotetracycline inducible expression of red ...

  20. Antibody Guide

    Type
    Collection
    ...but both rely on the same general strategy. Figure 5: Immunoprecipitation using bead-based antibody capture...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...
Showing: 21 - 40 of 40 results