We narrowed to 38 results for: cas9 genes

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert... Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require that each DNA target have...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... kits at Addgene contain multiplex-compatible gRNA plasmids, including: Multiplex CRISPR/Cas9 Assembly...which Cas has been integrated into the genome. Addgene has a large collection of empty gRNA vectors. Explore...CRISPR/Cas Toolkit for Plants CRISPR Resources Addgene has a large selection of other CRISPR plasmids ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA....gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification. Various epitope tags...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system . The...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...: Overview of NHEJ and HDR following cleavage by Cas9. Browse, sort, or search the tables below for CRISPR...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK ...Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials Kurt...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ...Bydder, S. F., Zhou, Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection...

...currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...A portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...

... vector for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression ...destination vector for gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection...host cell and are designed for a user to insert a gene of interest. They may contain tags or fusion proteins...These vectors allow you to test the function of your gene of interest in a controlled environment under various...we will assume that you want to express a gene; however, we have included a function section for if you...If you want to drive expression of your favorite gene, you will need a plasmid with a promoter that...mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient...

... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human...Gunawardane, R. N. (2017). Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell...Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window... The plasmids used to generate these lines have been deposited here with Addgene for distribution to the...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...

...excised, and gene expression proceeds. Gene Knockout: Placing the loxP sites on either side of a gene (called...shRNA expression. Gene Switch: These constructs contain two genes of interest, Genes A and B. When Cre... few popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with...LSL" cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre.... loxP"), will permit gene expression until Cre is present, at which time the gene will be disrupted or...Cre is absent, only Gene A is translated correctly. Cre expression excises Gene A and alters the reading...backbone to regulate your gene of interest. Depending on the construct, gene expression may be activated...

...and aggressive plan to use gene editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA ...mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR...diagnostic criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett...caused from loss-of-function mutations in the MECP2 gene. Causative mutations for Rett syndrome typically...global transcriptional regulator of thousands of genes and studies have suggested roles in transcriptional...activity dependent de-repression, chromatin remodeling, gene activation, and more. How loss of MECP2 protein ...suggests the major function of MECP2 is to regulate gene expression either locally or globally. The MECP2...

...promoter upstream of your gene of interest. If your gene of interest should be generally active and only turned...Tet-On Systems for Doxycycline-inducible Gene Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org...Tet-On system for regulated gene expression through viral evolution . Gene Ther, 13 (19), 1382–1390. https... methods to induce gene expression in experimental systems. 2nd and 3rd Generation Systems Subsequent ...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...repressible promoters are powerful tools to control gene expression and study biological targets. The tetracycline...

...knocking out genes or introducing specific mutations can reveal important insights into gene function. These...expression of a gene of interest in E. coli . The Marionette Sensor Collection (Addgene #1000000137) allows...of up to twelve genes using small-molecule inducers in the same E. coli strain. Addgene Blog Inducible ...bacterial gene biology or engineering metabolic pathways to synthesize a target metabolite, genetic tools ...visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering...transcriptional regulatory elements that will turn on gene expression upon protein–protein interaction. The...always advantageous to constitutively express your gene of interest at high levels. Perhaps high expression...

...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial genes. Molecular...of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under ...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed...

...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or ...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a ...Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator peptide can...

...protein (FP) tags. Information on genes and associated neurodegenerative diseases was curated from several...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...but not exhaustive. If you do not see a particular gene of interest, please search our full plasmid collection... Huntington's Disease Parkinson's Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized... T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416 25Q GPD HTT GFP...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry... Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a ...

...Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter...Nick ID Plasmid Gene/Insert Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter... ID Plasmid Gene/Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii...search the table for "Bacteria". Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Base... bacteria. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...