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We narrowed to 54 results for: cloning

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Showing: 21 - 40 of 54 results

  1. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors...Addgene #73345) is uniquely suited to simplify the cloning process as it can be used in blue–white screening...

  2. Plan Your Experiment

    Type
    Collection
    ...using standard restriction-ligation cloning. However, the exact cloning strategy will depend on the gRNA ...including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating...Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery Validate Genetic...the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down...few factors to keep in mind when designing and cloning your gRNAs. Select Genetic Element to be Targeted... about how to design your gRNA . Synthesize and Clone Desired gRNAs Once your target sequences are selected...selected, it’s time to design your gRNA oligos and clone them into your desired vector. In many cases, you...

  3. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common...

  5. Distribution to Industry

    Type
    Collection
    ... Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining...Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic...

  6. Worm Expression Resources

    Type
    Collection
    ...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...

  7. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...Backbones Find backbones or constructs to use for cloning to control expression of your gene of interest....EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with ...Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional... for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE...

  10. Synthetic Biology - Strains

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  11. Synthetic Biology - Metabolism

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  13. Zinc Finger Consortium Reagents

    Type
    Collection
    ... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

  15. Resolute Plasmid Collection

    Type
    Collection
    ...and SLC binder sequences in Gateway-compatible cloning vectors (pDONR, pENTR), bacterial expression vectors...NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning References Frommelt, F., Ladurner, R., Goldmann...

  16. Ras Pathway

    Type
    Collection
    ...Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0 mTOR Pathway p53 Pathway Background...

  18. Plasmid Collections

    Type
    Collection
    ... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...

  20. Retrovirus Plasmids

    Type
    Collection
    ...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression. Select with puromycin or screen...
Showing: 21 - 40 of 54 results