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We narrowed to 45 results for: crispr cas9 grna plasmid

Showing: 21 - 40 of 45 results

  1. Lentiviral Prep Service

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    ...information on CRISPR plasmids and function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...

  2. CRISPR Plasmids for Genomic Visualization

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Visualize CRISPR Plasmids:...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  3. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...search the tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression...interest in vivo. For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... CRISPR Purify CRISPR: Purify Genomic ...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  4. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and ...than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled...

  5. CRISPR Plasmids - RNA Targeting

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    ...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available for expression in...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ...

  6. Validated gRNA Sequences

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    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences...Addgene's CRISPR Plasmids and Resources page have been used to indicate the Cas9 application the gRNA was designed... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA Target...PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...Validated gRNA Sequence Datatable Target Gene Target Species Target Sequence Plasmid ID Application Cas9 Species...validated for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database...Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and...

  7. Zhang Lab CRISPR Page

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    ...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors ...CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery... 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...

  8. Worm Expression Resources

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    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs... insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some ...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use ...

  9. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening experiment...primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells...PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664...Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted...

  10. Zebrafish Plasmid Collection

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    ... zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones....Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...

  11. CRISPR Plasmids - Repress Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Interfere CRISPR Plasmids: Interfere...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  12. CRISPR Plasmids - Activate Gene Expression

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    ...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

  13. CRISPR Plasmids and Resources

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    ...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...variety of factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in ... . Drosophila CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for ...systems. Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed for use... in plants. Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed for use ...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...

  14. Neurodegeneration Research Collection

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    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...gene fragments, and gRNAs for genes linked to neurodegenerative disorders. Find plasmids expressing HTT, ...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...

  15. CRISPR Plasmids - Epigenetics

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    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian or...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  16. Genomic Deletions in Mammalian Cell Lines

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    ...the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...: This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is ...cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous...using CRISPR/Cas9 CRISPR... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...

  17. CRISPR Plasmids - CRISPR Transposases (CAST)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... or search the tables below for CRISPR Transposase plasmids. Plasmids are available for expression in ...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  18. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Nick CRISPR Plasmids: Nick...sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

  19. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression...CRISPR plasmids that create double strand breaks in DNA. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...

  20. CRISPR Plasmids - RNA Editing

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...tables below for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR... CRISPR RNA Editing CRISPR Plasmids...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...
Showing: 21 - 40 of 45 results