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We narrowed to 47 results for: crispr system

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Showing: 21 - 40 of 47 results

  1. Bacterial Expression Systems

    Type
    Collection
    ...with Addgene's CRISPR Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and...proteins. Addgene Blog CRISPR Methods for Bacteria CRISPR-based Genome Editing Systems For Editing in E. coli...Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein Visualization...tools like CRISPR-Cas9 are not only valuable but often essential. Find a collection of CRISPR plasmids ...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...Empty Backbones Collection Molecular Biology Guide CRISPR Guide Bacteria are commonly used to create, store...beyond that, researchers also use bacteria as model systems to answer many interesting biological questions...

  2. Cre-Lox and Other Site-Specific Recombinases

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    Collection
    ...Tumor Suppressor Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing...account for cellular toxicity in your system. Using inducible Cre systems in combination with lineage-specific...based on your expression system of choice, promoter, or search by inducible system (e.g., search for "split...about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control...Libraries/Kits Site-specific recombinase (SSR) systems, like Cre-lox, derive from fungi or bacteriophages...optimize these tools. The best characterized SSR systems, Cre and Flp, are tyrosine recombinases derived...include Dre, R, Nigri, SCre, VCre, and Vika. These systems work by sequential strand exchange at a specific...

  3. CRISPR References and Information

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    Collection
    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...

  4. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ...Plasmids are available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert...

  5. CRISPR Plasmids for Genomic Visualization

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    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... about visualization using CRISPR and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Visualize CRISPR Plasmids:...chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive... using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...

  6. Antibody Plasmid Collection

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    Collection
    ...Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin...Antibodies are large proteins made by the immune system that bind to an antigen, a particular part of a...bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies...-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic Human ...Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and Fab fragment...2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant... fragments in the form of scFvs A simple vector system to improve performance and utilisation of recombinant...

  7. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Cut CRISPR Plasmids: Cut Mammalian Bacteria... for CRISPR plasmids designed to introduce a DSB. To learn more about NHEJ, HDR, and other CRISPR topics...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that create double strand breaks in DNA. CRISPR...repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ...

  8. Rett Syndrome

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    Collection
    ...Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources...'s Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach...examples of additional CRISPR resources can be found below: Base Editing RNA Editing CRISPR-based DNA methylation...Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens in a new...recruitment of the NCoR complex for proper nervous system function. While there is wide variability in symptoms...Induced pluripotent stem cells (iPSCs) are a powerful system for generating Rett syndrome-specific differentiated...methylation editing system - Shawn Liu and Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights...

  9. Neurodegeneration Research Collection

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    Collection
    ...(Canman et al. Science. 1998) See More CRISPR Tools Find CRISPR pooled libraries available from Addgene... preps for systemic delivery of viral particles that will target the central nervous system, peripheral...neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors..., their families and caretakers, and the health system in general. The required care for these millions...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons...peripheral nervous system, vesicular brain cells and more. Measure synaptic transmission with improved glutamate... 2023) Initiate heritable gene silencing with CRISPRoff-v2.1 . (Nuñez et al. Cell. 2021) Conduct multimodal...

  10. Plasmid Collections

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    Collection
    ...plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors... Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent...Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology...pooled libraries. Pooled Library Types Barcoding CRISPR Screening Surface Display cDNA Depositor Collections...view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  11. Plasmids for Stem Cell Research

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    Collection
    ... Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human cells to...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski...four separate lentiviral plasmids A drug-inducible system for direct reprogramming of human somatic cells...Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse Oct4, Sox2, Klf4, and c-Myc...somatic cell. Sort the table by cell type, delivery system, species, or PI and then select the article link...

  12. AAV Viral Preps

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    Collection
    ...Transactivators, Affinity Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based...Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1, MaCPNS2...AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed on the...

  13. AAV Molecular Tools

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    Collection
    ...nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA...can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven...neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...can be used for cell ablation. ID Name Expression System Activity Serotype PI 45580 pAAV-flex-taCasp3-TEVp...for cell or protein labeling. ID Name Expression System Activity Serotype PI 149415 pAAV-TBG-ER-TurboID...various genes for overexpression. ID Name Expression System Activity Serotype PI 58376 pAAV/D377Y-mPCSK9 hAAT-driven...

  14. Distribution to Industry

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    Collection
    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...England Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium...

  15. Promega Plasmid Collection

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    Collection
    ...protein expression systems including E. coli , mammalian cells, and cell-free systems. NanoBiT Assays NanoLuc...regulation, tracking protein degradation, analyzing CRISPR-mediated gene editing outcomes, and conducting ...window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa) subunit...peptide, Small BiT (SmBiT; 11 amino acids). This system is ideal for protein:protein interaction (PPI) ...

  16. Synthetic Biology - Overview

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    Collection
    ... Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo...describes the rational engineering of biological systems for novel functions. Addgene is working with leading...

  17. Depositor Collections

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    Collection
    ...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression...Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort Gene Vector Core Viral Vectors - Oka ...

  18. Lentivirus Plasmids

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    Collection
    ...lentiviral plasmids to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression...Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector system. Expresses gag-pol and rev . Garry Nolan 8455 pCMV-dR8.2...pCI-VSVG VSV-G recommended for the FELIX (FIV-based) system. Garry Nolan 8454 pCMV-VSV-G VSV-G-expressing envelope...

  19. Botman-Teusink Yeast FP Collection

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    Collection
    ...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink...Resources External Resources Teusink and Bruggeman Systems Biology Lab website (Link opens in a new window...

  20. TALEN Plasmids and Kits

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    Collection
    ...also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important ...Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate...were optimized for TALEN expression in mammalian systems including hard-to-transfect cell lines. Features...transient double-selection of transfected cells, the system has successfully been employed for deletion mutagenesis...
Showing: 21 - 40 of 47 results