We narrowed to 41 results for: gfp control plasmid
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TypeCollection...Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent...Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing... the genome or control gene expression, and find plasmids for your experiments. Plasmid...Fluorescent Biosensors Chemogenetics Plasmid Collection Optogenetics Plasmid Collection Monosynaptic Neuronal...modify the genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre ,... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...
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Fluorescent Protein Guide: Biosensors
TypeCollection... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin Protein) for imaging...FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...interest in a controlled environment under various conditions. When choosing what plasmid backbone to use...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral... -
All Antibodies
TypeCollection...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...provide important details including the protein and plasmid sequence, the antigen sequence, and cross-species...develop and refine these application lists. The plasmids we use to produce antibodies are available as ...as part of Addgene’s larger Antibody Plasmid Collection , which also includes other types of affinity reagents...popular markers like tubulin or epitope tags like Myc, GFP, and more. Neuroscience : Antibodies targeting proteins...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or email... -
Worm Expression Resources
TypeCollection... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits...system for controlled gene expression in Caenorhabditis elegans. - Jane Hubbard Lab. Plasmids for the temporal... -
Biosensor AAV Preps
TypeCollection...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1...Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent...proteins, including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors... 140555 pAAV-hsyn-GRAB_DA-mut Syn GRAB_DA-mut (control) none Constitutive 9 Li 140556 pAAV-hsyn-GRAB_rDA1m...140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208698 pAAV-hSyn-GRAB-gDA3m...pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE GFAP iGABASnFR2 (control) none Constitutive 1, 5 GENIE 218874 pGP-AAV-syn-iGABASnFR2...pGP-AAV-syn-iGABASnFR2(no bind)-WPRE Syn iGABASnFR2 (control) none Constitutive 1, 5 GENIE 218877 pGP-AAV-syn-flex-iGABASnFR2... -
CRISPR References and Information
TypeCollection...lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO ...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled... -
Fluorescent Protein Guide: FRET
TypeCollection...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...these types of studies Addgene has a collection of plasmids suitable for creating individual fluorescently...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1... -
Recombinases AAV Preps
TypeCollection...recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more information .../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* Wilson 105558 pENN.AAV.CamKII...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....2, 5, 8, 9, rh10 Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre...EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB Wilson... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the above...pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...production , these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and... preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated...above serotypes and buffers. Please click on the plasmid name for ordering information. ID Name Promoter...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need.... -
Lentivirus Plasmids
TypeCollection... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd... empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table...packaging plasmids that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation... -
Antibody Plasmid Collection
TypeCollection...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...sort buttons to find antibody plasmids for: Common antigens such as GFP or mCherry Monoclonals, Nanobodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid... Plasmid Collections... -
Genetic Code Expansion
TypeCollection...Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All... -
Neurodegeneration Research Collection
TypeCollection... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...neurons in the substantia nigra, one of the movement control centers in the brain. The cause of this neuron ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... -
Trimmer Lab NeuroMab Collection
TypeCollection...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References...199419 Anti-GFP [N86/38R-2b] GFP Aequorea victoria Mouse IgG2b 199420 Anti-GFP [N86/8R-2b] GFP Aequorea ...206719 Anti-GFP [N86/38R-1] GFP Aequorea victoria Mouse IgG2a 206720 Anti-GFP [N86/8R-1] GFP Aequorea victoria...Rat Mouse 206743 GFP scFv [N86/44] N86/44 scFv GFP Aequorea victoria Mouse 206744 GFP scFv [N86/20] N86... Antibody Plasmid Collection NeuroMab Plasmid Collection... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target... -
Zebrafish Plasmid Collection
TypeCollection... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene...optogenetically controlled Cre/loxP system that enables precise temporal and spatial control of gene expression...expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections... -
COVID-19 Resources
TypeCollection...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection - Addgene has partnered...Bioworks to distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression... -
The Pleiades Promoter Project
TypeCollection... Plasmid Collections Pleiades Promoter Plasmids Pleiades Promoter Project The...using the links to view individual plasmid webpages. These plasmids are described in Portales-Casamar ...Negative Control N/A pEMS1312 cre N/A pEMS1301 cre/EGFP/NLS N/A pEMS1308 EGFP/cre N/A pEMS1302 EGFP/cre/NLS...N/A pEMS1306 EGFP/NLS N/A pEMS1307 EGFP/NLS N/A pEMS1313 intron-lacZ/NLS Positive Control CAG promoter...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective...human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below. Additional information ...pEMS1153 EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44... -
Kazuhiro Oka Lentiviral Vectors
TypeCollection...Adenoviral Guide Viral Vector Plasmids and Resources Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...vectors to express your gene of interest under the control of a variety of promoters. Professor Oka and the...promoter pCDH-EF1-copGFP 73030 Expresses copGFP from the EF1 promoter pCDH-EF1s-Nluc-P2A-copGFP-T2A-Puro 73032...pCDH-EF1s-copGFP 73034 Expressed copGFP from a truncated EF1 promoter pCDH-CMV-Nluc-P2A-copGFp-T2A-Puro...Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed... N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter...