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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 35 results for: his-tag

Showing: 21 - 35 of 35 results

  1. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...Available modifications include: histone acetylation by p300 histone demethylation by LSD1 cytosine methylation...for targeted epigenetic modification, including histone acetylation/demethylation, and cytosine methylation...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...NanoLuc® with either SNAP‐tag or HaloTag7 tag in which labeling of the self‐labeling tag with a fluorophore ...and Renilla luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase is ...biosensor that utilizes Renilla luciferase and a Halo tag to assay mRNA decay in real time. pKC-4.04, pKC-4.06...Lentiviral expression of firefly luciferase with a V5 tag Kevin Janes 83281 pAAV-CAG-FLuc Firefly CAG AAV expression...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (neo)...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian...systems. Each type of luciferase has advantages and disadvantages depending on the application. Firefly...

  3. All Antibodies

    Type
    Collection
    ...collections: Tags and Other Markers : Antibodies targeting popular markers like tubulin or epitope tags like ...species. We currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue...

  4. Validated gRNA Sequences

    Type
    Collection
    ...GCCACAAATCAGATTAATTTGG 64940 tag S. pyogenes 26355004 Mendenhall CREB1 H. sapiens GCCACAAATCAGATTAATTTGGG 64939 tag S. pyogenes...GAAAAGGATAATTGAGCCCCAGG 64254 tag S. pyogenes 26355004 Mendenhall GABPA H. sapiens TTTGGAGTCTCAGAATGTCCTGG 64255 tag S. pyogenes... 24967838 Mashimo ATF1 H. sapiens TAGGAATCAAACACTTTTATTGG 64690 tag S. pyogenes 26355004 Mendenhall ATM...pyogenes 25307933 Vale CEBPB H. sapiens CTCCGGCCACTGCTAGCGCGG 64036 tag S. pyogenes 26355004 Mendenhall CEBPB...CEBPB H. sapiens GCCGGCAGGGGGACGCGCGCGG 64047 tag S. pyogenes 26355004 Mendenhall Cent1 M. musculus GGCAACGTTTGACTTCCTGA...Xue RAD21 H. sapiens CCAAGGTTCCATATTATATAAGG 64057 tag S. pyogenes 26355004 Mendenhall rde-1(D718) C. elegans...rerio GTGCGCAACACATAGCACCA 65566 cut S. pyogenes 25849248 Du GFP A. victoria GAATAGCTCAGAGGCCGAGG 46914...

  5. CRISPR Guide

    Type
    Collection
    ...DNA. The system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The...in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...acid, turn a promoter on/off, or add small protein tags. Precise Modifications Using Homology Directed Repair...genetic modification ). Browse Plasmids: Endogenous Tagging CRISPR Base Editing To overcome low HDR efficiency...also be fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR ...dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing...sequence specified by a particular gRNA. Epitope tag(s) are fused to dCas9 or gRNA for efficient purification...

  6. CRISPR Plasmids and Resources

    Type
    Collection
    ...genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a given genomic locus. Visualize...discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...

  7. Brain Initiative Collection

    Type
    Collection
    ...Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used ...with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for...production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden...pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression...

  8. Optogenetics AAV Preps

    Type
    Collection
    ...PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV ...ChrimsonR (soma-targeted) GCaMP8s Cre dependent 9 Histed 183519 pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ... adds a myristoylation sequence and flag-epitope tag to each ORF from the labs of William Hahn and Jean...-A major histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex,...major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major histocompatibility complex...HLA-DOB major histocompatibility complex, class II, DO beta DOB HLA-DPA1 major histocompatibility complex, ...major histocompatibility complex, class II, DQ alpha 2 HLA-DXA HLA-DQB1 major histocompatibility complex...major histocompatibility complex, class II, DR alpha HLA-DRA1 HLA-DRB1 major histocompatibility complex... HLA-G major histocompatibility complex, class I, G MHC-G HLA-H major histocompatibility complex, class...

  10. Trimmer Lab NeuroMab Collection

    Type
    Collection
    .../9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [N77...206528 Anti-Histone H3 pThr11 [N123/19R] Histone H3 pThr11 Rat Mouse IgG2a 206529 Anti-Histone H3 pThr11...206550 Anti-Synaptotagmin-5 [N274/8R] Synaptotagmin-5 Mouse Mouse IgG2a 206552 Anti-Histone H4-dimethyl-Arg3... Human Mouse IgG2a 114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3... Human Mouse IgG2a 114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a 114483 Anti-GluA1...) Rat Mouse IgG2a 114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin...28R] HCN3 Mouse Mouse IgG2a 177500 Anti-6xHis [N144/14R] 6xHis Mouse IgG2a 177501 Anti-TrpV3 [N15/4R] ...

  11. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...CODA CODA aGTCCGCCTCCACCCGAGGTAGAG zgc:111821 OZ597 and OZ598 CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890...gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg zgc:153086 OZ511 and OZ512 OPEN OPEN ...cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l) OZ519 and OZ520 OPEN OPEN...aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523 and OZ524 OPEN OPEN gCTCCCAACCactttGCTGGTGTCa...and OZ548 OPEN OPEN gAGCTCCGTCtcgctgGCGGCCGAAg histamine receptor H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa...tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg NRF2b (nfe212b) OZ561 and OZ562 OPEN ...tGTCTCCGGCagaggGAGGAGGAGa esrrga OZ563 and OZ564 CODA CODA tAGCTACCGCTGGCGTCTGAGGAGc HACE1 OZ565 and OZ566 CODA CODA gCTCATCTCCACCGAGCTGGAGAAg...

  12. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...apoeb TAL3402 & TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA arap3a TAL3026 & TAL3027...ARSA TAL 3002 & TAL 3003 TCAAGAGGCGGTCTCCCTctcaacgagaccaccaTAGCCGAGGTGCTGAAGA asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA...cited2 TAL3228 & TAL3229 TGGTAGACCGCATGATGGcaatgaaccatggtcgTTTCCCAGATGCTGTGAA cited4a TAL3046 & TAL3047...clcn1b TAL3052 & TAL3053 TTTTATTGTGTTACAGACtgcctagcccgtgtccAGAGGTATATTGTGACAA col22A1 TAL3410 & TAL3411...crispld2 TAL3232 & TAL3233 TATCAGGAGGAGCTAGAAcccaacagcactaaacCCGACGCTCCTGTCCGAA cx52.6 TAL3056 & TAL3057...CYP20A1 TAL3414 & TAL3415 TAGACTTTCCTGAAAAGCaaaaatgctagattttGCCATTTTTGCTGTGACA dachc TAL3234 & TAL3235 ...TCCCGTGCAAGGAATGAAtgcaaaaacatttcctTTCCCTTGTTTTGACGGA dcps TAL3416 & TAL3417 TGAACAGTCCACCGATAAcccagtggtagacttcCAGCTCACCCGCATTCTA ddx26b TAL3418 & TAL3419...

  13. p53 Pathway

    Type
    Collection
    ... can be inherited or arise through exposure to mutagens like radiation or viruses (e.g., HPV, the human...TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins...of p300/CBP in p53-dependent signal pathways. Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine...

  14. Lentivirus Plasmids

    Type
    Collection
    ... genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems...plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of...
Showing: 21 - 35 of 35 results