We narrowed to 71 results for: mpl

...parts can be combined to construct complex networks. This is accomplished through the use of assembly standards...meaning that the suffix will need to be reverse complimented for primer design. The restriction enzymes used...encodes Gly-Ser in-frame with prefix start codon; Employs high-efficiency enzymes. Prohibited Restriction...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham...lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the viral glycoprotein...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...

...microscope, or detect when the protein is present in a sample — just to name a few. Traditional sources of antibodies...individual clone targeting a single antigen. An example of R-mAbs is the NeuroMab collection — monoclonal...single-domain antibodies (also known as nanobodies). For example, scientists often use plasmids encoding scFv fragments...recombinant antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation...

...Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric...allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer Information...Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using...using the materials in the order. After the RSA is complete, Addgene will send you a copy of the MTA, which...

...help advance science while reducing operational complexity. This unique collection contains plasmids developed... below to find plasmids in the collection. For example, search by response element, reporter, protein,...Link opens in a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT...LgBiT; 18 kDa) subunit and a small complementary peptide, Small BiT (SmBiT; 11 amino acids). This system...

...Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final...bind adenenine with lower specificity). With the template for studying this type of technology already laid... bp. TAL effectors have some restrictions (for example, the target must start with a T), but they still...

...ARF GTPase family, information on each gene, and examples of plasmid types available for each gene. Click...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375 181 Bacterial... and GEF family, information on each gene, and examples of plasmid types available for each gene. Click...request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 (ARF1GAP) 55738 ...

...constructing CRISPR gene-editing vectors in just two simple cloning steps. CRISPR Kate Creasey Krainer , Vladimir...system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome editing logistics. ...planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC) and Förster Resonance Energy Transfer...MoBiFC Kit Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein...

...techniques, including that it is easy to implement due to the simplicity of gRNA design, programmable for different...copies of labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently...

...cited in publications. All pricing includes DNA amplification, viral vector production, purification, and ...Our production process and quality control, with examples of quality control data. Help Center Articles ...will also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that...

...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex, comprised of a combination of ...difficulties in getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3...

...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR resources, along with links...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...

...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority...variation and lifestyle or environmental factors. For example, variations of Apolipoprotein E (APOE), such as...collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link opens in...

...interacts only with their own target sites (for example, Cre with lox sites, Flp with FRT sites, Dre with...preferred codon usage will improve expression. For example, iCre or FLPo in mammalian cells may facilitate...translocation of a gene of interest. Using Cre or Flp as examples, here are a few popular recombinase-dependent ...

...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...

...transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely...compatible vectors for bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium...Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions...

...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...

...factor isoform has a unique 24 bp barcode that simplifies identification of the transcription factor isoform...is uniquely barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI...

...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...