... one set of primers internal to the sequence to be deleted (“non-deletion band”) and another set of primers... Gordanpour A, Nam RK, Sugar L, Bacopulos S, Seth A. J. Vis. Exp . 2012. PubMed . Western blotting...

...out in the spring of 2011, the groups describe a set of customized plasmids that can be used with the ...Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN...

... top Resources RAS Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the...

... neomycin cassette Zebrafish, Sea urchin, Xenopus, and C. elegans Hamdoun Lab Plasmids - Set includes ...GFP-variants and non-GFP proteins that result in a diverse set of colors. Addgene has assembled a collection of ...mCarmine in pcDNA3 - Mammalian Expression mCarmine in pRSETB - Bacterial Expression mCardinal 604 659 17 5.3...Mammalian Expression pPAGFP-C1 - Mammalian Expression pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR... / 581 47 / 30 5.6/6.4 Prone to oligomerization pRSETa mEos2 - Bacterial Expression pFA6a-link-yomEos2...

...single gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse...single or pooled amplicon deep sequencing, WGS datasets and allows the direct comparison of individual...upload. Currently supports: Human, mouse, rat, marmoset, pig, chicken, frog (X. tropicalis and X. laevis...cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA:...

...to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce...

... lacZ in response to green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids...fluorescent protein variant (CyPet) found in pCyPet-His . pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger...

...Conformationally destabilized Firefly luciferase mutants : A set of three sensors that are increasingly structurally...biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created by fusing a bright...

...invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages infecting ...

...Targeted gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...

...Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...

...research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository....

...non-biological replicates) often each require their own set of controls. Multiplex Assays Flow cytometry, IF,... cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires...requires single-antibody controls, a small subset of the sample labeled with only one antibody, for each ...

... early-onset, where symptoms appear between a person’s thirties and mid-sixties, or late-onset, where ...individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It is more likely... results in death within 3-5 years from symptom onset. Disease mechanisms are still poorly understood ...during or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases...cases of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation in...such as the ε4 allele, are a risk factor for late-onset AD. Many more genes have been associated with Alzheimer's...

...SLC1A3 CMV Episodic ataxia Susan Amara 32868 SETD1A SETD1A His T7 Parkinson's Cheryl Arrowsmith 32880 FLAG-MATR3...Parkinsonism, early-onset Roger Reeves 53822 SYNJ1-170_pCSdest SYNJ1 CMV, SP6 Parkinsonism, early-onset Roger Reeves...ALS Catherine Tomasetto 104450 pEGFPC1-hVAP-B KD/MD VAPB GFP, HA CMV ALS Catherine Tomasetto 104465 hnRNPA2...SERPINI1 Halo Dementia Michael Ward 178169 SETX_Halo_C_allele SETX Halo ALS, Ataxia with neuropathy Michael...C6A/C111A SOD1 ALS Nam-Chul Ha 193056 pADC10-SETXhel SETX Flag CMV, p10 ALS, Ataxia with neuropathy Andrew...infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His Spinocerebellar ataxia, infantile onset Smita...disease Kivanc Birsoy 218838 3xFlag-eGFP-Flag-SETX SETX Flag, GFP CMV ALS, Ataxia with neuropathy Albert...

... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369...gRNA module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or ...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...

...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730... plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned ...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...

...plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins. The ... The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and gRNA...PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To ...cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for... expression vector allows for efficient marker cassette FLP-out. Plasmids can be found associated with...

...active promoter like CAG, or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre...p252 pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green 8408 p255 pPr-CREM/Ins...Ins/CMV-STOP-luc CREM and CMV-STOP-luc cassette, separated by insulator probasin Mammalian Green 11543...Sauer 12168 pMB80 (R26-CreER) Cre-ERT2 with loxp cassette; Targeting vector for Rosa26 locus Mammalian Jacks...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...activates gene of interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration...pPNT6 Cre activates gene of interest, removes Neo cassette. Used in gene targeting. Mouse Targeting Shivdasani...

...inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged...inducible expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional...conditional cell line generation; contains expression cassette for TetR-P2A-GFP; see article for additional constructs... rtTA On Alexeyev 14901 pCL-CTIG The inducible cassette contains tTA at its 5' end and an inducible promoter...