We narrowed to 68 results for: tec
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TypeCollection...of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...bp from the predicted cleavage site to ensure detection would not be impacted by a small indel at the ...in Molecular Biology. 2001. PubMed . MicroRNA detection in prostate tumors by quantitative real-time PCR...PubMed . Western blotting: sample preparation to detection. Eslami A, Lujan J. J. Vis. Exp . 2010...
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Tetracycline Inducible Expression
TypeCollection...binding of Tet-Advanced transactivator (developed by Clontech, based on Resnitzky et al., 1994). TRE3G promoter...alternative effectors for Tet transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04...promoters with minimized background expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10...opens in a new window) Tet Systems: Science and Technology (Link opens in a new window) Do you have suggestions... -
AAV Viral Preps
TypeCollection...can be safely handled at the lowest biosafety protection levels. High quality and high titer in-stock ...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed... -
Institute for Protein Innovation
TypeCollection...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...collection includes anti-glypicans that accurately detect glypican presence and explore glypican function...that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse or search... -
Plan Your Experiment
TypeCollection...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID:...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase... -
Antibody Plasmid Collection
TypeCollection...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample--just to...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system... -
Distribution to Industry
TypeCollection... Proteins Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein...intellectual property rights, and provides liability protection for material providers. Addgene's deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information... -
Plant Plasmids and Resources
TypeCollection...spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation...CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ... -
Zebrafish Plasmid Collection
TypeCollection...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ... -
TALEN Plasmids and Kits
TypeCollection...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091... -
Rett Syndrome
TypeCollection... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window... -
Zinc Finger Consortium Reagents
TypeCollection...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of... -
Antibodies
TypeCollection...you choose and use antibodies, with an expert technical support team ready to answer your questions. All... antibody production and storage techniques, and explain common applications. View Guide ... -
Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids
TypeCollection... genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their...use in mammalian cells SpCas9 variants with no detectable off-target effects Modified PAM specifity of ... -
Viral Prep Service for Plasmids and Pooled Libraries
TypeCollection...Viral Vector Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...troubleshooting advice and explanations of viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors... -
Resolute Plasmid Collection
TypeCollection...inclusive, ‘open-access ethos’ to the results, techniques and reagents with the highest-possible quality... research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project... -
CRISPR Plasmids - Prime Edit
TypeCollection...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA - additional protection added ... -
Microbiology Resources
TypeCollection...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...