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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 60 results for: uros

Showing: 21 - 40 of 60 results

  1. Retrovirus Plasmids

    Type
    Collection
    ... expression; also expresses GFP. See pMKO.1 puro for Puro resistance. Hahn 63704 pRetroX GFP T2A Cre CMV...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen... immortalized cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676 pMKO...CMV/MSV pRXTN is a modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-... for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid; deletion...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... plasmids with Neomycin selection Puromycin Mammalian Tet-pLKO-puro - Tet-inducible lentiviral...Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning...plasmids for Drosophila expression pMT-puro - Inducible gene expression under...mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1...lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA...lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...targeting efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n...2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A...) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR ...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed...

  4. Luciferase Plasmid Collection

    Type
    Collection
    .... Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling...firefly luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly...Venus-Aka-luciferase Roland Friedel 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of Renilla luciferase...luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of Venus-firefly...for bioluminescent chemo-optogenetic control of neuronal signaling. TRUPATH Kit , ONE-GO Biosensors Kit...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    ...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...production of transgenic zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct...resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - A repository...) for the distribution of zebrafish lines from European researchers. International Zebrafish Society (...

  6. Optogenetics Plasmid Collection

    Type
    Collection
    ...specific neurons, often utilizing viral expression systems, to activate or deactivate these neurons in a .... When using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent... table below for the optogenetics plasmids for neuronal excitation and inhibition. For example, search...

  7. AAV Molecular Tools

    Type
    Collection
    ..., Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments...Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV...

  8. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222...pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL...pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen Lab SapTrap...

  10. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Site-specific...events but can improve specificity in targeted neuronal populations. Read our blog post about how to minimize...Plasmids Advanced Uses of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids...blog Advanced Uses of Cre-lox and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids...

  11. Caltech Systemic Capsids

    Type
    Collection
    ...tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism... tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For ...: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1,...animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with...

  12. Chemogenetics Plasmids

    Type
    Collection
    ...receptors. Chemogenetic receptors are popular in neuroscience, where they can be used to interrogate brain... function and examine the relationship between neuronal signaling and behavior. Depending on the signaling...the opsin to either excite or inhibit the target neuron. For more detailed descriptions of chemogenetic...

  13. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Filaments (neuronal) Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999...Autophagosome LC3(ATG8) mCherry Maria Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru...

  14. Resolute Plasmid Collection

    Type
    Collection
    ...the Innovative Medicine Initiative (IMI) of the European Union. The RESOLUTE mission is to develop data...This Joint Undertaking receives support from the European Union's Horizon 2020 research and innovation programme... Resources Brain Initiative Plasmid Collection NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning...

  15. Antibody Plasmid Collection

    Type
    Collection
    ...R-mAbs is the NeuroMab collection — monoclonal antibodies extensively validated for neuroscience research ...research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently...

  16. Depositor Collections

    Type
    Collection
    ...Special Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection...Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection Rett ...

  17. AAV Viral Preps

    Type
    Collection
    ...Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1,...

  18. All Antibodies

    Type
    Collection
    ...Myc, GFP, and more. Neuroscience : Antibodies targeting proteins for neuroscience research. Institute ...

  19. Plasmid Collections

    Type
    Collection
    ...MJFF) Plasmid Collection Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Rett...

  20. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...repression system can be found in this article: Vigouroux, A., Oldewurtel, E., Cui, L., Bikard, D., & Van...different populations. Image and caption from Vigouroux et al., 2018, under a CC BY 4.0 license....
Showing: 21 - 40 of 60 results