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We narrowed to 54 results for: ARA-2

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Showing: 41 - 54 of 54 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ... and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein.... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  2. Institute for Protein Innovation

    Type
    Collection
    ... proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand pairs bridge...IPI is providing access to high-quality, well-characterized antibodies in an open science model. Learn more...

  3. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...15264811 Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal...

  4. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons... the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector Hillel...for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable...for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...hSyn promoter rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter...expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link...and the tet-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals...tissue-specific promoter and administer the transgene separately. All-in-one system: express both the transactivator...(Link opens in a new window) External Resources Takara: Tet-inducible Systems Overview (Link opens in ...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids. ID...

  7. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...AAV2 vectors in the mouse retina. Mol Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: ...sequencing of the final viral vector preparation. These viral vector preparations were produced with the pTR-UF11...Service , Addgene is producing ready-to-use viral preparations from the University of Florida for enhanced ...-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers...injection. It was identified by John Chiorini and characterized by Shannon Boye’s lab. AAV44.9(E531D) The AAV44.9... of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID...Shannon Boye and cite Boye, et al. 2016. Impact of Heparan Sulfate Binding on Transduction of Retina by Recombinant...

  8. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2 and Cap2... (E4, E2a, and VA), eliminating the need for a separate helper plasmid during AAV production. You can ...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisomes Peroximal targeting signal (SKL) mCherry...Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles...GolgiCFP Golgi apparatus eNOS(1-33) CFP Alexandra Newton 36205 pmTurquoise2-Golgi Golgi apparatus B4GALT1(1...pmScarlet_Giantin_C1 Golgi apparatus Giantin mScarlet Dorus Gadella 129630 mTurquoise2-Giantin Golgi apparatus Giantin mTurquoise2...Golgi apparatus Giantin td5StayGold Atsushi Miyawaki 231777 pCMV-mGold2t-MannII-N-10 Golgi apparatus MannII... Golgi apparatus MannII mGold2s Francois St-Pierre 189773 pmScarlet3-Giantin_C1 Golgi apparatus Giantin...subcellular structures. To compare with a well-characterized marker's localization pattern, you should fuse...

  10. Fluorescent Proteins: FRET

    Type
    Collection
    ...referred to as intermolecular or bimolecular FRET) or 2) conformational changes within a protein where the...protein-protein interactions where each protein is separately fused to a donor or acceptor molecule (also referred...pairs with mTurquoise2 Mastop et al. (2017) Characterization of a spectrally diverse set of fluorescent...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...different inherited genes: Presenilin 1, Presenilin 2, and APP. The majority (> 90%) of individuals develop...Neurodegenerative diseases are a group of disorders characterized by the progressive degeneration and ultimate...biology of these diseases, rather than focusing on separate diseases, will help speed progress towards curing...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression... Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - ...and cloning of fusion proteins, along with key parameters to help you plan your experiment. Tables are ... like multicolor imaging with better spectral separation. Protein Excitation (nm) Emission (nm) Brightness...

  13. Retrograde AAV viral preps

    Type
    Collection
    ...sensor Jonathan Ting 83899 pAAV-mDlx-GCaMP6f-Fishell-2 mDlx GCaMP6f expression under the control of the mDlx...final viral vector preparation. In some cases, in vivo testing of these viral preparations was performed by...Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova...David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid...-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Karl Deisseroth...pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Karl Deisseroth...GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Jonathan Ting 51083...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo...Tet) Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector ...astrocyte-selective expression in AAV Dual promoter Separate expression pRGEB31 - Deliver sgRNA and Cas9 into...
Showing: 41 - 54 of 54 results