...target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which ...RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining single-stranded... binding of RISC. For more information on shRNA design and delivery, see resources below. Figure 1: Overview... Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab...Taxman, D. J. (2010). Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods...

...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ... to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID ...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...

...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently repurposed...protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair complementary region to any...ampicillin resistance, wherein the N20 can be custom designed to target arbitrary sequences in the genome. Co-expression...sgRNA cassette, wherein the GN19 can be custom designed to target sequences in the genome. The pgRNA_humanized...targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as an RNA-Guided Platform...can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically inactive...

... cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, ... like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. coli strains for protein expression...! Plasmid ID Promoter Epitope Tag(s) ID Purpose pDEST-HisMBP 11085 Tac (lactose/IPTG inducible) MBP, 6x...and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG inducible) MBP...molecule, 3OC6HSL, from a promoter repressed by EsaR. Designed to be used with the pAC-EsaR Series . pAC-EsaR...Christopher Voigt When combined with pPLPCB(S), encodes a green light responsive sensor protein that activates...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has been...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information...information about HDR/repair template design. How to use CRISPR to tag your gene of interest Mendenhall and...interest is currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein...protein to your target sequence, as well as design and clone in the homology arms for the donor plasmid. The...produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair template...

...Robert Singer 27382 pDEST/N1-hEB1-GFP Microtubules EB1 GFP Robin Shaw 40908 pDEST/LIfeAct-mCherry-N1 Actin...Davidson 40907 pDEST/hCx43-EGFP-N1 Gap Junctions Connexin 43 EGFP Robin Shaw 49861 pDEST-mCherry-GJA1-20k-N1...encoding fluorescent proteins tagged with genes or peptides with known subcellular localization patterns. ...important to determine where your protein of interest resides. There are a variety of methods for determining...junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers...

...RGN target sites and to facilitate design of short oligonucleotides needed to construct gRNA expression...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( ...cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated...Engineering newsgroup here . Links to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression...

...genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding ...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites continue...have been the focus of biomedical research for decades. Recent developments with CRISPR-Cas9 gene editing...

...signaling during development. The IPI collection includes anti-glypicans that accurately detect glypican... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

...Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to target various...pyogenes , S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, PAM requirements, experimental...unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and function...

...efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description Expression System...Cre expression plasmids. Addgene's viral service provides high-quality and high-titer viral preparations... are currently in our repository. ID Plasmid Description Promoter Expression System PI 8394 p209 pCMV-... 61573 Rasgrf2-2A-dCre targeting vector DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian ...Eisenhoffer 131783 pTol2-CreLite CreLite; Tol2 destination vector with mTagBFP2 Zebrafish Eisenhoffer 131785...Vivid photodimers lacUV5 Bacterial Dunlop 135217 pDEST mfap4:icre-p2a-tomato iCre and tdTomato mfap4 Zebrafish...to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico Cre activates...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful tool...screening experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any ...can be found in the following table, which also includes information about the type of genetic modification... represent all 4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-wide Inhibition Library...2 40,964 Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition Yeast Ingolia NA ~6 44,580...Knockout Human Mali 3rd 1 74 Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A...

...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...effector and TALEN kits here . References Efficient design and assembly of custom TALEN and other TAL effector-based...

... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...2A-Hygro-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells for single-cell...Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for single-cell...Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for single-cell...

...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets....Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems,...mutation reduces off-target effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in Xenopus. CRISPR...in Xenopus. Cut Fully functional Cas9 enzymes designed to introduce a double-strand break (DSBs) at a specific...

... chemogenetic receptors: Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) and Pharmacologically...inhibit the target neuron. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you...

...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - Wildonger...production of RNA from the T7 promoter, while pMB70 is designed for in vivo transcrition controlled by the regulatory...-chiRNA plasmid via the BbsI restriction sites. Design oligos based on the following template: example...non-model invertebrate species. These plasmids are described in: Genome engineering of Drosophila with the ...

...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...

...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in C. elegans. CRISPR... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...