We narrowed to 105 results for: ESC;

...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

... in a new window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview This collection... form an active enzyme and generate a bright luminescent signal in the presence of a substrate. NanoBiT... detection. It can be paired with LgBiT for bioluminescent assays or with the high-affinity anti-HiBiT...

...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...

... packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging...for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral...

... for gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...Lentiviral Packaging Plasmids ID Plasmid Generation Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector...Popular Lentiviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

...2023) See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...

...UGA). The amber codon is the rarest codon in Escherichia coli , which makes it the most commonly used ...Liu 164081 pCMV-MfPylRS (human-opti) PylRS M. flavescens acetyl-lysine Mammalian Tao Liu 164195 pCMV-MtAcKRS...Liu 164196 pCMV-MfBulKRS (human-opti) BulKRS M. flavescens acetyl-lysine Mammalian Tao Liu 164579 pEVOL-...non-canonical amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites changes to UAG...

...Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein ...

... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3...and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...

...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment.... mRNA and Proteins In addition to the methods described above, you can also directly deliver mature mRNA...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to... plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication ...

...biological context. Assays monitoring impedance, fluorescence, mass/charge properties, protein thermal stabilization...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

...10.1038/nprot.2009.98. Epub 2009 Sep 17. PubMed . Description Use of the OPEN method requires OPEN pools (available...using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature...

...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...

...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...

...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....

...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell...