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We narrowed to 155 results for: Gene

Showing: 41 - 60 of 155 results

  1. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...methylation Epigenetic modification is typically targeted to a specific promoter or enhancer for your gene of ...CRISPR Epigenetics CRISPR Plasmids: Epigenetics Mammalian Plant Resources To make targeted epigenetic modifications... plasmids designed for epigenetic modification. To learn more about epigenetic modification with CRISPR...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include: P300 — activation... local chromatin state. Figure 1: Overview of epigenetic modification using CRISPR. Created with BioRender.com...

  2. Chemogenetics AAV Preps

    Type
    Collection
    ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral...in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information... Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See What You’re Looking For? Our Packaged...

  3. Optogenetics AAV Preps

    Type
    Collection
    ...Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools can be used... potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information...ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...select plasmids in the repository. Browse our optogenetics AAV collection, including channelrhodopsins,...control cell activity in living tissue with light. Optogenetics is typically used in neuroscience to control...consider using fewer filtering options. Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with...

  4. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252... CRISPR knockdown to modulate expression of two genes without cross‐talk. (A) Schematic of the strain ...

  5. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared...research. This collection is part of Addgene's Neurodegeneration Research Collection , which includes ...Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools (Link opens ...

  6. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does...dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. KLF Research Plasmids

    Type
    Collection
    ...depositing plasmids with Addgene or email deposit@addgene.org to get started. Genes KLF gene pages with information...KLF Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...to the establishment of this shared resource on Addgene. If you have created plasmids containing KLF, we...

  8. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI ...PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  9. Microbiology Resources

    Type
    Collection
    ...page to look for specific genes or applications that are not listed here. Addgene Microbiology Resources ...of Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye...Lab Synthetic Biology BIOFAB plasmid set : E. coli gene expression - Endy Lab CIDAR MoClo Parts Kit : Molecular...Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast ...Resources : Browse plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio...Resources for Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...Resources Prime editing is a “search and replace” gene editing method in which a reverse transcriptase ...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...BFP Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  11. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table contains a general list of plasmids ... to the reliance of the AAV on certain exogenous gene products that mediate AAV replication. This requirement...without the use of a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g...Addgene AAV transfer plasmids Viral...disease in humans. For these reasons, AAVs are generally contained at lower biosafety levels and elicit...AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can ...

  12. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...vectors for human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 ...cells by a mutant adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye...Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. PMID: 26390090... Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID:...: Viral Vectors As part of our Viral Service , Addgene is producing ready-to-use viral preparations from...preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in...

  13. Plan Your Experiment

    Type
    Collection
    ...mutant allele of a gene (point mutant)? Increase or decrease expression of a target gene? Once you have a...want to: Generate complete and permanent loss of gene expression or function (knockout)? Generate a specific...cloning your gRNAs. Select Genetic Element to be Targeted The exact region of the gene you target will depend...Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells will use error-prone non-homologous...Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without permanently modifying the genome...cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently modifying the genome...Intereference : Target the promoter region of the gene of interest, where the Cas9 protein can block transcription...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi...each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target cells ...are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR library may target...target the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR...Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman...Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini ...Birsoy 3rd 7–10 2,105 630 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy 3rd...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's Current AAV inventory... from the Penn Vector Core. Chemogenetics (including DREADDs) Optogenetics Recombinases Calcium Sensors... are now available for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040...tdtomato Optogenetics Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda...mCherry.WPRE.SV40 Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl...eYFP.WPRE.hGH Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...EYFP Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl...

  16. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Request: Scope of Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and... If Addgene determines that our current queue is too high and we do not expect to meet the general turnaround...with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs QC measures...produced through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL ... to toxicity or other reasons For these cases, Addgene will promptly notify you by email within a few ...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...not yield the titer guaranteed here or by email, Addgene will contact you to offer some options for moving...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ... information, please visit the Addgene SAM library page . Targeted gene activation using SAM There are...available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed...contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...human codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid... and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV...-Cre recombinase and sgRNAs targeting the mouse genes: Kras , p53 , and Lkb1 . This plasmid also contains...

  18. Lentivirus Plasmids

    Type
    Collection
    ...viral particle) and toxicity of the gene product (i.e., if the gene encoded by the transfer plasmid is ... which can be modified to encode a wide range of gene products. The main restrictions on a lentiviral ...chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan...gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions of ...pULTRA 3rd bi-cistronic expression of EGFP and the gene of interest, also can express shRNA (cloning H1-...mutagensis. The 2nd and 3rd generation lentiviral systems available at Addgene have been designed for increased...used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253...

  19. Cancer Research Plasmids and Resources

    Type
    Collection
    ... RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled... click on a component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by ...of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor and tumor...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer...

  20. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert... Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 41 - 60 of 155 results