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We narrowed to 88 results for: URE

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Showing: 41 - 60 of 88 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols, 2016 . Yamamoto ...very rapidly after the addition of auxin to the culture medium. Plasmids can be found associated with the...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmid and the effects on gene expression can be measured by the luminescence output. The most popular types...substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase is secreted and...altered emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...

  3. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...-expressing BirA biotin ligase, as seen in the CAPTURE system . The locus is subsequently isolated by ...associated with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using...

  4. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...found in bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared...many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created ...

  5. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...regulation and developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...

  7. Resolute Plasmid Collection

    Type
    Collection
    ...2020 research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...is embedded in an integrated knowledgebase that features, among others, genetic variants and disease associations...

  9. Mammalian RNAi Tools

    Type
    Collection
    ...shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference....silence: conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link opens in ...

  10. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... change. Adenine DNA deaminases do not exist in nature, but have been created by directed evolution of...to activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort...

  11. E11 Bio PRISM Collection

    Type
    Collection
    ...driver for brain circuit mapping and connectomics. Figure 1: Reconstruction of a sparse set of neurons across...high-resolution optical imaging down to individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled...

  12. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...for distribution to the research community. Key features: Allows for visualization of multiple proteins...these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone...

  13. Viral Vector Guides and Plasmids

    Type
    Collection
    ...genome integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral...plasmids). When transfected into in vitro cell cultures, these plasmids direct the production of viral...

  14. Distribution to Industry

    Type
    Collection
    ... Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled...Libraries Antibodies Ordering and MTA Information Featured Collections NEW NanoLuc and HaloTag Fusions from...

  15. Arf GTPase Family

    Type
    Collection
    ...GTPase signaling activity. Figure 1: Locations of ARF superfamily members. Figure from Szutl, et al., 2019...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...transparency of the embryo and larvae, it is easy to culture large populations that are amenable to genetics...combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous single-cell profiling of lineages...

  17. COVID-19 Resources

    Type
    Collection
    ...Collection (Link opens in a new window) Springer Nature SARS-CoV-2 and COVID-19 Articles (Link opens in... F. (2020). The proximal origin of SARS-CoV-2 . Nature medicine, 26 (4), 450–452. https://doi.org/10.1038...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ... vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial gene clusters S. cerevisiae...

  19. Caltech Systemic Capsids

    Type
    Collection
    ...Center at Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. ...Information When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru...

  20. Brain Initiative Collection

    Type
    Collection
    ...dynamic picture of the brain generated through this effort will provide novel ways to treat, cure, and even...
Showing: 41 - 60 of 88 results