We narrowed to 96 results for: eng
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TypeCollection... TALengineering Reagents TALEN Expression Vectors TALEN Expression... FLASH assembly methods can be found on the TALengineering website . All three of these methods yield ...
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CRISPR Plasmids and Resources
TypeCollection...immune system to a revolutionary tool for genome engineering. CRISPR Topic Page : Want the latest news on ...experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas enzymes generate double strand... -
Microbiology Resources
TypeCollection...gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles...Read our CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making... -
Zebrafish Plasmid Collection
TypeCollection...and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular...drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific... -
Open Enzyme Collection
TypeCollection...functionality or add new functions through genetic engineering and directed evolution. More information about...polymerase 165543 pOpen-Bstpol Bst DNA Polymerase, Full Length 165544 pOpen-T5gene122 T5 DNA Polymerase 165553... -
COVID-19 Resources
TypeCollection...Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized ...Center The Lancet COVID-19 Resource Centre The New England Journal of Medicine Coronavirus webpage PLOS Journals... -
CRISPR References and Information
TypeCollection... Manuel Leonetti Lab . For more information, see Peng et al. (2024) (Link opens in a new window) . gRNA...new window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids... -
Plan Your Experiment
TypeCollection...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient...manuscript here: Bauer et al, 2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA... -
Tetracycline Inducible Expression
TypeCollection... control gene expression, the system has been engineered with a variety of improvements. A synthetic Tet...systems (Loew et al., 2010; Heinz et al., 2011), engineered to eliminate binding of endogenous mammalian ... -
CRISPR Pooled gRNA Libraries
TypeCollection...192824 Knockout Mouse Knouse 3rd ~5 71,878 Mouse messenger-RBP sgRNA Library 169082 Knockout Mouse Turner...Knockout Yeast Borodina N/A 1 56 Turner Lab Human messenger-RBP sgRNA Library 168791 Knockout Human Turner... -
Plasmids for Stem Cell Research
TypeCollection...Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng RNA Human Reprogramming factors for delivering synthetic...expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused ...Patricia Wadsworth 27123 eTC GFP beta-actin full length Actin Filaments beta-actin EGFP Robert Singer 27382... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection... Gene, 1992 ), and since then scientists have engineered numerous GFP-variants and non-GFP proteins that...Photoactivatable (e.g. off to on) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission... -
Genetic Code Expansion
TypeCollection...Because there are no free codons, this can be challenging. In E.coli , the rarest codon is the amber stop...the ncAA in the media to make sure that the full length protein is only made when the ncAA is included.... -
Synthetic Biology - Browse Plasmids
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse the list of available plasmids related... -
Synthetic Biology - Algal
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Bacterial
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Fungal
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Mammalian
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...