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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 98 results for: genome

Showing: 41 - 60 of 98 results

  1. Synthetic Biology - Metabolism

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene...Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector. The PITCh donor plasmid...vectors to insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid...-programming sgRNA and HR donors for selectable genome modification. This system can be used to create...

  4. Zinc Finger Consortium Reagents

    Type
    Collection
    ... these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish...expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents...

  5. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces...

  6. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...

  7. Retrovirus Plasmids

    Type
    Collection
    ...gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic proteins, ...This provirus then gets integrated into the host's genome by the accompanying integrase protein. When scientists...γ-retroviruses integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional...

  8. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Plasmids: Yeast Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ... Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering... Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like...target DNA sequences in living cells (TALE-mediated Genome Visualization [TGV]). pTALYM3 and pTALYM4 contain...this page are designed to target and edit the epigenome of putative enhancers in mammalian cells. These...target locus ( Mendenhall et al., 2013 ). This epigenome editing was also shown to alter regulation of ...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... prime editor plasmids for (search and replace) genome editing....Prime Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...designed with a spacer that binds to a specific genomic DNA locus and directs the nickase to the target...

  11. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...recombinant AAV does not integrate into the host genome, transgene expression can be long-lived. The utility...conjunction with the plasmid that encodes the viral genome (typically called cis -plasmid or transfer plasmid... a general list of plasmids that encode the AAV genome (i.e., the genetic information that will be contained...

  12. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Plasmids: Xenopus Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...and can be used to faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication...

  13. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Plasmids: Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...

  14. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Plasmids: Cut Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...

  15. TALEN Engineering

    Type
    Collection
    ... Genome Engineering TALEN Kits TALengineering Reagents ...TALengineering website. Users may also wish to join the Genome Engineering newsgroup. An upgraded version of the...REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome editing...

  16. Resolute Plasmid Collection

    Type
    Collection
    ...the largest family of transporters in the human genome with approximately 450 identified members. Many...RESOLUTE mission. The combination of metabolomics and genome engineering forms the core of the deorphanizing...

  17. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...: C. elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...

  18. AAV Molecular Tools

    Type
    Collection
    ...TEVp for studying cell ablation. 5 Shah , Wells Genome Engineering Tools These AAV encode Cas9 nuclease...nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype...

  19. CRISPR Plasmids - Plants

    Type
    Collection
    ...Plasmids: Plants Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...

  20. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Browse All SynBio Assembly Standards SynBio Blog Genome Engineering A distinguishing feature of synthetic...Allows for in-frame fusions; NheI is rare in E. Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI...
Showing: 41 - 60 of 98 results