...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection...

...includes 35 plasmids expressing a variety of fluorescent sensors to monitor cell physiology. RNA-based aptamer... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...S-adenosyl methionine . Reporters of miRNAs Toehold sensors for various target RNAs Explore the Fluorescent Biosensor...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...

... Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional... editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR... search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert...human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External Resources Research...

... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies. Antibodies.... Recombinant expression validation A recombinant expression strategy is used to express the protein of...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary in... comparison of expression between different samples, by normalizing protein expression to the controls...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...

...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian Expression AcGFP1 475 505 28 Prone ... - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression Topaz-C1 - Mammalian Expression Topaz-pBAD - Bacterial Expression mTopaz 514 527...

...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein...cytokines and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK...a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be helpful...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...

... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic...resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species...Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids to perform your experiment...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica...

... may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources Addgene Blog: ...: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...

...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ...129 KB Sontheimer gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and...597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330)... for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription...endogenous proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21... generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180...

... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...'s worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the...

...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti...

...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 159,111 plasmids . We've...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion... Expression Kits Kits are groups of plasmids that have been packaged together because they are intended...

...SepOTSγ Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid...SepOTSθ Plasmid 68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 ...68294 SepOTSν Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP...Amp) Plasmid 68300 MBP-MEK1 Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68305...pKD-SepRS-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ... 68306 C321.ΔA.Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep...( 188537 ) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

...our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights plasmid collections... Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made...created by cloning antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast...collections that can be used to create and express plasmid-based antibodies. Description Article PI One-step assembly... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...

...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...chains. Antibodies are expressed for 7 days and then purified from the supernatant using Protein A or Protein...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

...Receptor Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene Description AR plasmids AR, RP11... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection... Nuclear Receptor plasmid collection for the community. If you have created plasmids that you would like...ESRRB Plasmids ESRRB, DFNB35, ERR2, ERRb, ESRL2, NR3B2 estrogen-related receptor beta ESRRG Plasmids ESRRG...group A, member 1 PGR Plasmids PGR, NR3C3, PR progesterone receptor PPARA Plasmids PPARA, SC22CB-5E3.5,...receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha RORA Plasmids RORA, ROR1, ROR2...orphan receptor C RXRA Plasmids RXRA, NR2B1 retinoid X receptor, alpha RXRG Plasmids RXRG, NR2B3, RXRC retinoid...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Example of viral targeting of cells expressing Cre AND Flp, here expressed through a double transgenic animal...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...and VCre to enable viral expression of molecular payloads in target populations defined by multiple parameters...understanding of neural circuit functionality. Precisely expressing these tools in well-defined cellular sub-populations...

...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs...fragments, and gRNAs for genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...and target species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy: ...bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...

... will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate...charge the tRNA with a different amino acid. They are typically expressed from a single plasmid, with multiple...Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA synthetase for...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place where... Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...