...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ... BsaI In vitro transcription none, need Cas9 plasmid Joung pMB70 cU6 BsaI Transfection none, need Cas9...vitro transcription none, need Cas9 plasmid Boxem PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...Calarco SP6-sgRNA-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg L4440_BioBrick-sgRNA...

...easily and inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture...leader sequence (L) for translocation of the heavy chain across the ER membrane. PCR-mediated fusion of...leader sequence (L) for translocation of the light chain across the ER membrane. Downstream of the insert... Mouse IgG2a 206524 Anti-SNAT1 transporter [N104/37R] SNAT1 transporter Rat Mouse IgG2a 206525 Anti-Ankyrin-G...206668 Anti-VGlut2 [N29/29R-2b] VGlut2 neurotransmitter transporter Rat Mouse IgG2b 206669 Anti-Slo2.2 K...206706 Anti-VGlut1 [N28/9R-1] VGlut1 neurotransmitter transporter Rat Mouse IgG1 206707 Anti-VGlut2 [N29...[N29/29R-1] VGlut2 neurotransmitter transporter Rat Mouse IgG1 206708 Anti-Arl13b [N295B/66R-1] Arl13b...

... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor... Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton... p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B, Harris CC. Science. 1991 Jul ...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...56,762 (Set A) 56,476 (Set B) Broad GPP activation Caprano p65-HSF 92383 (Set A) 92384 (Set B) Activation ...164084 Knockout Human Sabatini 3rd 10 7,501 Human Membrane Protein Activation Library 113345 Activation Human...Knockout Human Superti-Furga 3rd 6 2,609 Human Transcription Factor Knockout Library 162275 Knockout Human...Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout...159393 Knockout Mouse Moffat 3rd ~5 19,463 Mouse Transcription Factor Activation Library 122131 Activation ...

...Tetracycline Transactivators Affinity Purification Cell Ablation Tetracycline Transactivators and Inducible...from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP)....-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...ihSyn) Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

...efficiently block transcription (in bacteria, ~300-fold repression) by interfering with transcriptional elongation...for selection or fluorescent gating of transiently transfected cells. Co-expression of both plasmids in...elongation, RNA polymerase binding, or transcription factor binding. The binding specificity is determined jointly...the nuclease-deficient mutant could silence transcription on the gene expression level without genetically...CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut..., Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS...

...Link opens in a new window) . Transfections are performed using the transfer plasmid, a plasmid encoding...cell cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and ...section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...opens in a new window) ). Briefly, 293T cells are transduced with serial dilutions of a lentiviral vector,... taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching...integrated provirus using PCR. This assay involves transducing cells with serial dilutions of the lentiviral...

....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications... pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein 163505 CN1390...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

... Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may be useful for detection assays...site for furin proteases.( 3 ) BSG - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily...proteolysis of SARS-CoV Spike protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease... method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published...Kellner, et al. bioRxiv 2020.06.23.166397 reverse transcriptase, protease, and Bst polymerase constructs now...unpublished) Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa...S, et al. bioRxiv 2020.03.29.013342 Reverse Transcriptase plasmids from Andrew Ellington's lab. High-surety...

...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light...anti-GFP antibody), the target antigen is first transiently expressed in mammalian cells. To learn which ... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...

...with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control...Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A Control Plasmid...Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control...pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector...Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid...

...including dual-transgenic recombinase-expressing mouse lines 2-9 and combinations of transgenic recombinase-expressing... Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image...Online spreadsheet of Flp-recombinase-expressing transgenic mouse and rat lines. Plasmids In addition to ... Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal...Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PMID...Krook-Magnuson E. 2019. A novel population of long-range inhibitory neurons. bioRxiv:554360 Yu K, Ahrens ...Psychiatry 24(9):1351-1368. PMID:30755721 Mandelbaum G, Taranda J, Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi...

...identified by depositors and/or when BRAIN Initiative grants are noted in publications associated with Addgene...-GfaABC1D-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent... expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE...

... (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...emerged as a model system for human disease and translational research, including the study of cancer, inflammation...-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi...zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development...examples of popular tools to use in zebrafish, ranging from optogenetics to tools for studying diseases...vectors designed to streamline the production of transgenic zebrafish for studying neurodegenerative disorders...

...CRISPR Blog Posts CRISPR-on is an RNA-guided transcriptional activator system with two components, the dCas9...sgRNA). While the dCas9 protein provides the transactivation activity, the sgRNA component provides the ...dCas9 activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA ...Gateway donor vectors [48218~48221] and can be transferred to Gateway destination vectors (e.g., pmax-DEST...endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H,...H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Oct;...

...glycoprotein (G) gene, which is not required for the transcription or replication of the genome within infected...infected cells, but is required for transynnaptic spread (Wickersham et al., 2007a, Wickersham et al., 2007b...essential G protein is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell... cell using the G-protein, which is supplied in trans . (3) Nascent G-protein coated RAVB is released ...Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

...reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the...cases Rett syndrome can be inherited. In females, random X inactivation results in approximately half of...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...2-M-2xStrep-IRES-Puro Expression of SARS-CoV-2 membrane (M) protein Krogan 141385 pLVX-EF1alpha-SARS-CoV...Service Suggestion Form . Please note this does not guarantee viral service, but lets us know what viruses would...

...used to transform iPSCs and fibroblasts into neurons and more. New and Noteworthy: Study aberrant axon initial...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases...kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods. 2023 May 4. See...PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, and cortical neuron...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...