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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 88 results for: IRE

Showing: 31 - 40 of 88 results

  1. CRISPR Plasmids - Plants

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...template to introduce specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter ...a prime editing guide RNA (pegRNA). The pegRNA directs the nickase to the target site by homology to a...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions...

  2. CRISPR Plasmids - Yeast

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...template to introduce specific edits via homology-directed repair (HDR). ID Plasmid Description Gene/Insert...of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions... FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat Commun... with peroxiredoxin-based probes Real-time monitoring of basal H 2 O 2 levels with peroxiredoxin-based...cellular locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded sensor and ...11/520/eaah3738. Justin Blau cAMP (cyclic AMP) cAMPFIRE cAMP biosensors with improved sensitivity Sensitive... PLoS One. 2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse fluorescent biosensor for citrate (Citron...mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors...mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive Serotonin...

  4. Worm Expression Resources

    Type
    Collection
    ...Wormbuilder website. General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C....characterized which allow researchers to express desired constructs in precise cell types and/or developmental...plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert ...modified approach has also been developed by the Fire laboratory and described in Efficient Marker-Free...vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without PATC, which diminishes...

  5. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...can continue for many kilobases in one or both directions from the target region, effectively turning Cas3... Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. ...other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mT-sapphire 399 511 Monomer (A206K) mT-Sapphire-N1 - Mammalian Expression mT-Sapphire-C1 - Mammalian...Structure Plasmids T-sapphire 399 511 26 4.9 1.3 hr Prone to dimerization pFA6a-link-yETSapphire-CaUra3 - Yeast...Mammalian Expression mT-Sapphire-pBAD - Bacterial Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K...

  7. Rett Syndrome

    Type
    Collection
    ..., is regression of previously acquired skills, notably loss of acquired purposeful hand movements and ...Envigo. The following cell lines are available directly from the labs in which they were created. Find...rsrt.org The following cell lines are available directly from the labs in which they were created. Find... a p.R168X or p.T158M MECP2 mutation, and the direction and degree of skewing of X-chromosome inactivation...

  8. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...removing the amplification and extraction steps required when one receives plasmids in bacterial stabs....yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning...DNA is designed for use in cloning applications. Direct transfection into cells is not recommended. Can...independently including the check out and will require separate shipping charges. Where can I find cloning...

  9. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions...plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids....

  10. Serotype Testing AAV

    Type
    Collection
    ...for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid 37825...37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information about...
Showing: 31 - 40 of 88 results