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Showing: 61 - 80 of 143 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...For small deletions (as the “deletion band” may still amplify), resolve agarose gel to determine if size...for 20 min). These cycling conditions allow for digestion and ligation to occur in one reaction (see step...conditions for each primer pair designed based on testing the bulk sorted cells. Run samples on 2% agarose...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout ...Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon Human Cas13 lncRNA...69,716 SAM v2 - 2 plasmid system 1000000078 (Blasticidin) Activation Human Zhang 3rd 3 70,290 CHyMErA ...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection FRET Biosensors...-pmCherryC1 Actin Filaments Cortactin mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin Filaments...Protein Collection . Return to top Do you have suggestions for other plasmids that should be added to this...

  4. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...partnership to provide AAV viral preps, includes listing of transfer status for viral preps....Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available) Based...Wilson Please contact [email protected] with any questions. Return to top...

  5. Neuroscience

    Type
    Collection
    ...Reactivity Recommended Applications PI Do you have suggestions for other antibodies that should be added to ... represent the official views of the National Institutes of Health....

  6. Depositor Collections

    Type
    Collection
    ...- Davidson Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science ...

  7. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...mammalian cells or zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One...mammalian cells or zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One...

  8. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent iRFP variants have been...photoactivation) 3.0 pPAiRFP2-N1 Do you have suggestions for other plasmids that should be added to this...

  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...on how it was originally used. Here are a few questions to consider when choosing a gRNA: Does a given...Insert Vector Type PI Publication Do you have suggestions for other plasmids that should be added to this...

  10. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... and Cascade on one single plasmid, though many still separate the individual Cas components. Figure 1...Last reviewed on: January 30, 2025 Do you have suggestions for other plasmids that should be added to this...

  11. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...cloning to insert the desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ...

  12. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes of...Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast...

  13. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...please let us know using the Cloning Grade DNA Suggestion Form . Please note that this does not guarantee...us of which plasmids would be most helpful to requesting scientists. Return to top...

  14. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...viral vector production is a common step after requesting plasmids from Addgene, and by providing this ...storage. Biosafety Guide Documentation for your institutional biosafety office and your lab, information on...

  15. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... BbsI, BsaI Ingestion by C. elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids...

  16. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... weeks after the approval of the MTA by your institution. Our final step of the QC process, viral genome...communicated by email will supersede the general estimated turnaround time above. After starting viral production...

  17. Adenovirus Plasmids

    Type
    Collection
    ...Adenoviral plasmid construction Frequently asked questions about adenovirus Glossary of commonly used adenovirus...adenovirus genomes Bunz Return to Top Do you have suggestions for other plasmids that should be added to this...

  18. Retrovirus Plasmids

    Type
    Collection
    ...γ-Retroviral packaging systems Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope...mammalian cells Wong Return to Top Do you have suggestions for other plasmids that should be added to this...

  19. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3...Esp31 for PBS-RT No BFP Ervin Welker Do you have suggestions for other plasmids that should be added to this...

  20. Plant Plasmids and Resources

    Type
    Collection
    ... simplifies gene silencing and genome editing logistics - Jose-Antonio Daros. Return to top Empty Backbones... RNA-seq analysis. Return to top Do you have suggestions for other plasmids that should be added to this...
Showing: 61 - 80 of 143 results