We narrowed to 120 results for: prot

...antibody, CRISPR, disease research, visualization, protein expression, synthetic biology, and viral vector...Collection Visualization Michael Davidson Fluorescent Protein Collection Allen Institute for Brain Science AAV... Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase...Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection...

...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA — additional protection added ...PE2 plus additional sgRNA PE4 — PE2 plus MLH1 (a protein component of MMR) mutant to inhibit MMR PE5 — PE3...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

...can be safely handled at the lowest biosafety protection levels. We offer 1,077 in-stock viral vector ... your AAV experiments, remember to share your protocols and results through Addgene’s Data Hub . By contributing...globe and shared our well-developed and trusted protocols and peer-reviewed techniques (Link opens in a ...preps. Data Hub Access Addgene’s Data Hub for protocols and results shared by other scientists using AAV...

...technology for targeting enzymes and other useful protein domains to a specific DNA sequence ( Figure 1A ...Biotechnology in August, uses a serial ligation protocol to assemble arrays. Dr. Joung, co-founder of the...TAL effector arrays in a 2012 issue of Nature Protocols . More information and help can be found at TALeffector...Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas DF. Science...

... and capsid proteins necessary for AAV vector production and assembly. The capsid protein encoded in the...production and assembly of viral capsids. The capsid protein encoded in the packaging plasmid will determine... Videos Addgene's Biosafety Resources Addgene Protocols General Transfection AAV Production in HEK293T...

...drug-inducible approach may also be used. Split proteins: The recombinase is split into inactive N- and...fragments in the same cell reconstitutes a functional protein, allowing recombination of the DNA between target...Drosophila Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing... Reporter Plasmids Incorporating a fluorescent protein into your expression vector allows you to visualize...

...Browse mitogen-activated protein kinase (MAPK) plasmids....Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated... kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse cellular programs...

...Resources CRISPR requires you to express both a Cas protein and a target-specific gRNA in the same cell at ...cells, but are limited to the function of the Cas protein (cut, nick, activate, interfere, etc.). gRNA-only...reference page . CRISPR Guide Lentiviral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

...boundary defined by anti-CRISPR proteins or running into other proteins bound to DNA, and the break can...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual...Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources...

...Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web...and degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target...

...editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both types of...inhibitor (UGI) or the bacteriophage Mu- derived Gam protein (Mu- GAM). While many base editors are designed...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...useful worm CRISPR resources, along with links to protocols, can be found below: SapTrap-SEC kit for C. elegans... expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window...

...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ...Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. ...plasmids in our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose ...

...fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein Activity Cre...

...antibodies targeting epitope tags and common fusion proteins.... target epitope tags and other common cellular proteins. These ready-to-use recombinant monoclonal antibodies...

...recombinant antibodies targeting neuroscience-related proteins....currently available from our catalog that target proteins popular in neuroscience research. These ready-...

...reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium members like...reagents for engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories...

...production process by sharing our protocols and quality control methods. Learn More Guide ...Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids? Browse our collection...

...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...