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We narrowed to 139 results for: Ide

Showing: 81 - 100 of 139 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...cloning to control expression of your gene of interest. ID Plasmid Description Transactivator Promoter PI 21915... the transactivator for your tetracycline system. ID Plasmid Description Transactivator PI 26429 pLenti...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible...Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids Inducible and repressible promoters...repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions...importantly, the TetR protein has been modified to provide two classes of tet-responsive systems: Tet-Off ...1995, Gossen et al. used random mutagenesis to identify the amino acid residues of TetR that were important...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP and puromycin... the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous ... . Use these tools to help identify guide sequences that minimize identical genomic matches or near-matches...CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present a video reprint from the Journal...oligos for each guide and its associated reverse complement including additional nucleotides for cloning ...Add “CACC” before the 20-mer guide sequence and “AAAC” before the guide’s reverse complement for cloning...Journal of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs...

  3. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population...Caltech Systemic Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are ...

  4. Chemogenetics Plasmids

    Type
    Collection
    ... AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Plasmid Collection You may also like... Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics... Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled protein... your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... ) VPR ( Addgene #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed...utilizing one of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This process... shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski...in the context of the developing mouse retina. To aide in their studies of the regulatory networks involved...

  7. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to ...

  8. Mammalian RNAi Tools

    Type
    Collection
    ...components, like argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources...

  9. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional...protein with many available isoforms and mutants provides a shared experience for the research community...

  10. AAV for Neuronal Tracing

    Type
    Collection
    ...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell population of...deletion-mutant rabies, the essential G protein is provided to the starter cell in trans , via delivery by...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top ... encoding scFv fragments that bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP...antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following...

  12. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector...Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role...MCP tagged plasmids for visualization of proteins inside cells. Depositor...ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated ...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...are useful for labeling secreted proteins with disulfide bridges such as antibodies. MCP-tag is a mutant...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement...See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information about this field. Receptor... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

  14. AAV Packaged on Request

    Type
    Collection
    ... plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

  15. Brain Initiative Collection

    Type
    Collection
    ...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...of the brain generated through this effort will provide novel ways to treat, cure, and even prevent brain... the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative grants...jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A. Mark Histed 179459-AAV1 pAAV-EF1a-DIO-JEDI...available at Addgene. AAV Viral Service . Addgene provides a viral service to help facilitate scientific ...

  16. Genetic Code Expansion

    Type
    Collection
    ...contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI...to enhance non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites...modified codons for unnatural amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past...from the tRNA is incorporated into the growing peptide chain. To expand the genetic code, modified tRNAs... the genetic code, there are several things to consider. If you are working off of a previously established...

  17. Validated gRNA Sequences

    Type
    Collection
    ...Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...in the associated article (listed below by PubMed ID). These factors include: Does a given gRNA sequence...gRNA Target Sequences You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...in Addgene's database as well as from sequences provided to us by users (see below for more details). Keep... Keep in mind that additional factors must be considered before using a validated gRNA sequence in your...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    .... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top ...rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater fish...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ...CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung...scientists in Latin America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A...zebrafish-specific gene expression database that provides information about when genes are active in developing...

  19. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970 pTR-...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...retina following subretinal injection. It was identified by John Chiorini and characterized by Shannon...

  20. Cre-lox system

    Type
    Collection
    ...of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression System PI...Empty backbones to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico...selecting desired clones, and making transgenic mice. ID Plasmid/Article Description Expression System PI ... Cre; NeoR act15 D. discoideum Kay 108996 pDM1489 Cre; HygroR act15 D. discoideum Kay 109378 LZF40: LZF40...Cre expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special features...Cre expression plasmids. Addgene's viral service provides high-quality and high-titer viral preparations...65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and ...
Showing: 81 - 100 of 139 results