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...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement... in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information...physically separate) none 1, 9, PHPeB Jordane Dimidschstein 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

... plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven serotypes...optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this service...

...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...research. Browse the BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...dissemination to the research community. Browse plasmids , viral preps , and antibodies created with support...distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as high-quality...control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2-dTom-nlsdTom AAV vector...

..., and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description ... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources...some of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright...: Find plasmids for use in zebrafish. Plasmid...

...click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970 pTR-...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep 15...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...where the vector is diluted by the physiologic body fluids, such as blood, vitreous, CSF, etc. Not suitable...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Many of these plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase...codons for non-canonical amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI Return to top Bacterial...to enhance non-canonical amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic...Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids Bacterial Strains The genetic code for...incorporation of amino acids beyond the 20 standard ones, known as non-canonical amino acids (ncAAs). This strategy...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the...

...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI ...and accessory plasmids available from Addgene for generating your own specific TALE plasmids. Genome...TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...Musunuru/Cowan Lab TALEN Kit Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains... 38142 RCIscript-GoldyTALEN Daniel Carlson The plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed...Golden Gate TALEN 2.0 36033 pTAL5-BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative...

...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...regulatory dTomato Constitutive 1, 9, PHP.eB Jordane Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory EGFP...EGFP Constitutive 1, 9, PHP.eB Jordane Dimidschstein 135641 pAAV-S5E6-dTom-nlsdTom E2 regulatory dTomato...dTomato Constitutive 1 Jordane Dimidschstein 137135 pAAV-Ef1a-oScarlet EF1a oScarlet Constitutive 8 Karl Deisseroth...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Lab Improved Phosphoprotein Synthesis Reagents Type ID Item Strain 192872 rEcoli XpS (MG1655-C321 mutS+,... other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express... central phosphoserine site flanked by 15 amino acids on either side occurring within the parent protein...

...bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid...MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett...mammalian CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few...gene. Use the search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial...

...Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...electrical potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information... ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...ChR2 mCherry Constitutive 1, 9, PHPeB Jordane Dimidschstein 137142 pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2...C1V1 EYFP Constitutive 1, 5, 9, PHPeB Jordane Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine...GCaMP6f none Constitutive 1, 9, PHPeB Jordane Dimidschstein 137122 pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research Reprogramming Plasmids Differentiation...Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells are proliferative, unspecialized...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...Ottosson Stem Cell Research Plasmids This table contains a general list of plasmids that have been used in ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...

...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice... Cas9 gRNA plasmids from the Zhang lab Genome... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR... CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab... Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR ...Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse...involved in defense against invading phages and plasmids. CRISPR loci in microbial hosts contain a combination...

... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene’s repository ...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses...viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or check ...out our additional microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute...

... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection...Your Perfect Plasmid Backbone Empty backbones are plasmids containing only the elements needed for replication...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent...marker Also see more empty backbones on our Plant Plasmids and Resources collection page Yeast GAL4, PGK,...

...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Several different tagging techniques, as well as the plasmids and protocols needed for using them, are described...Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag (currently...based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed repair...arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were tested ...

...available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are ...more plasmids containing the necessary components of the viral genome to assemble the viral capsids (packaging...packaging and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct the production...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...information to cells. These vectors rely on production plasmids that have been carefully optimized for both safety...vectors and find viral production and transfer plasmids for a wide array of research purposes. Interested...

...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...variety of factors. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...