...Protein Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting... 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin...

... Depositor Collections Rinehart Lab Phosphoprotein Reagents...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated... via partial UAG codon reassignment and release factor 1 deletion. Heinemann IU, Rovner AJ, Aerni HR, ...

...basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN...basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal...TARDBP His, GST, Factor Xa cspA ALS Aaron Gitler 27452 pCold TDP43 G294A TARDBP His, Factor Xa cspA ALS Aaron...M337V TARDBP His, Factor Xa cspA ALS Aaron Gitler 27454 pCold TDP43 Q331K TARDBP His, Factor Xa cspA ALS Aaron...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd Bukau 169713 A1-LCD WT HNRNPA1...107R] VAPB CMV ALS James Trimmer 188167 Anti-IP3 receptor, type 1 [L24/21R] ITPR1 CMV Spinocerebellar ataxia...

... Depositor Collections Cell Migration Consortium (CMC) Plasmids...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome... Depositor Collections IGVF Consortium Impact of Genomic Variation...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF...

...Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... and type V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that...orientation matters. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB...

...is a Monomer not a Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...molecules to activate genetically engineered cellular receptors that affect signalling pathways within cells. ...

...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

...antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected with...Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend... suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested...some of our other resources to help you choose, store, or use your antibody. Antibody Guide Antibody Protocols...

...s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add ...sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion of a G nucleotide ...Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4 )... ( e.g., with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells ...

...Modular Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

...are expressed and purified from plasmids in our repository....summarize antibody production and storage techniques, and explain common applications. View...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...catalytically dead Cas13b creates a programmable RNA editor that converts adenosine to inosine in RNA. Since...effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-984-1090 ADAR2 truncation retain...

...and Cas9 expression vectors and validated the use of RNAs produced by these vectors for efficiently modifying...oligonucleotides needed to construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase... a specific locus, avoiding known transcription factor and other protein binding sites. Browse, sort, ...

...gRNA expression vectors available from Addgene....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...

...Collection of adenovirus shuttle vectors and strains...Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of medium-sized...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

...Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco...promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619 pLB 3rd Modification of...promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730 pLionII 3rd FIV-derived...

...sites changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306...tolC, and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair instead...amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI...