...click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970 pTR-...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...retina following subretinal injection. It was identified by John Chiorini and characterized by Shannon...

...of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression System PI...Empty backbones to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico...selecting desired clones, and making transgenic mice. ID Plasmid/Article Description Expression System PI ... Cre; NeoR act15 D. discoideum Kay 108996 pDM1489 Cre; HygroR act15 D. discoideum Kay 109378 LZF40: LZF40...Cre expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special features...Cre expression plasmids. Addgene's viral service provides high-quality and high-titer viral preparations...65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and ...

...Individual plasmids can be ordered via the links below: ID Plasmid Description 47948 3xFLAG-dCas9/pCMV-7.1 enChIP...Expresses a guide RNA (gRNA) to target human IRF-1 promoter 61080 gRNA-hIRF-1 #12/pSIR Expresses a guide RNA ...Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...genomic regions retaining molecular interactions and identify molecules interacting with those regions. Locus-specific...catalytically inactive form of Cas9 (dCas9) plus guide RNA (gRNA) for biochemical purification (Fig. 2)...interest. 98134 hIRF-1 gRNA #409 (Sa) Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the...system. 105283 hIRF-1 gRNA #351 (Sa) Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the...

...Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN...You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important...repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying documentation...

...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection... found to match all requested criteria. Please consider using fewer filtering options. Browse all Control...

...Lab Improved Phosphoprotein Synthesis Reagents Type ID Item Strain 192872 rEcoli XpS (MG1655-C321 mutS+,... Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria for... for proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski...Substrate Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Barber KW, Miller CJ, Jun...serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“phosphosites...phosphoserine site flanked by 15 amino acids on either side occurring within the parent protein. Using the recoded...purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...

...bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return...toddlerhood primarily affecting females at an incidence of approximately 1:10,000. It is characterized...for proper nervous system function. While there is wide variability in symptoms even within patients with...window) PMID: 1606614 1999 - Huda Zoghbi's lab identifies MECP2 as the genetic cause of RTT. (Link opens...the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ... Rett syndrome in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in...

...AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... See our Optogenetics Plasmids and Optogenetics Guide page for more information about this field. Use ... found to match all requested criteria. Please consider using fewer filtering options. Browse all Optogenetics...

...Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-... found to match all requested criteria. Please consider using fewer filtering options. Browse all Retrograde...

...Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species...4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-wide Inhibition Library 113134 — ...Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406 Human improved genome-wide library v1 ...genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide library...Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral Mouse Genome-wide CRISPR...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...Adamson 3rd ∼3 366 or 1,573 Activity-optimized genome-wide library Discontinued Knockout Human Sabatini and...

...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

... R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species Isotype...produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...IgG2a 188173 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R] NPY2R/Neuropeptide Y receptor type 2 Human...Parvalbumin Rat Mouse IgG2b 206639 Anti-Neuropeptide Y [L115/13R-2b] Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT... 211594 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/106R-2b] NPY2R/Neuropeptide Y receptor type 2 ...Rat Mouse 190505 NPY/Neuropeptide Y scFv [L115/13] L115/13 scFv 2t NPY/Neuropeptide Y Human Mouse 190506...

...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor... HBGF8, HBNF, NEGF1 PYY peptide YY PYY1 QRFP pyroglutamylated RFamide peptide 26RFa, MGC119794, P518 RABEP1...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...

... series of links and guides to help you use CRISPR in your lab. Learn More Guide to CRISPR technology ...Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively...gene families, HDR oligonucleotide design, and batch processing for designing genome-wide gRNA libraries.... for identifying specific Cas9 targets in genomes From the Church Lab , a program that identifies gRNA...References and Information You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...entries describing enzyme activity, experimental considerations, and more. CasPEDIA is a community resource...The amplicon sequence expected after HDR can be provided as an optional input to assess HDR frequency. ...

... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...selection marker to identify and enrich positive cells Packaging and envelope plasmids provide the necessary...see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA in a wide variety of mammalian... desired edit, ideally less than 10 bp away. In this case, it is necessary to identify the exact location... increase specificity, you can also consider using a high-fidelity Cas enzyme. In addition to off-target...the genome like never before. This section will provide a general framework to get you started using CRISPR...apply to using CRISPR in other organisms. First, consider the genetic manipulation that is necessary to ...

...along with the guide RNA. For most applications, we recommend using Cas9 with the single guide RNA (sgRNA)...that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting efficiency...PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9...2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461;...nickase) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate...that adding an additional guanine nucleotide to the 5’ of 21-bp guide can increase targeting efficiency...

...NIH provides basic guidelines for working with various types of viruses; however, these guidelines may...engineered to harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic ...viruses for a wide array of research purposes. Which Type of Virus Should I Use? This table provides a brief...Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide University ...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . References Cohen S, ... Also Like... Addgene's Viral Service Biosafety Guide Blog: Plasmids 101-Viral Vector Elements Viral Vectors...Plasmids Which Type of Virus Should I Use? Use this guide to learn more about the different types of viruses...

...display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9...Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. ... Genome Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...crRNAs). Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic...but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages... screens. Pooled gRNA libraries can be used to identify genes that are important to a given phenotype....sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR ...

...usually pick 3-6 guides around the region to find the most efficient guide (most guides would work, but...and the concentration of Cas9 and guide RNA (gRNA). These considerations only matter if the PAM sequence... HR template vector design guidelines? A few notes below are considerations for designing HR donor. Generally...plasmid, clone in your target guides, test run them and then select the best guide to co-transfect in HR donor... 100bp away, ideally less than 10bp away. For small (<50bp) changes, you should consider using ssDNA oligo...for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric gRNA... drive expression, is it necessary to add a G nucleotide to the start of my target sequence? The human...

...Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor...GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular...isoforms, individual links to each gene page are provided below. Color is used for clarity and does not ...isoforms, individual links to each gene page are provided below. Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine...activating protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin...factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP EIF4EBP1 EIF4EBP2...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...