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...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. To find emtpy...the help of Scott Lindner . Cut ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top ...

...comprehensive human transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two ...scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive...

...sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for expression in mammalian cells or ...) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric...

...Empty gRNA expression vectors for insertion of custom gRNA target sequences...., Xenopus, Worm, or Other. Whether the plasmid contains Cas9 and if so, which function of Cas9. We have... as Puromycin or EGFP Cloning enzyme used for insertion of your gRNA sequence, such as BsbI or BsaI Cas9...

...prep and how to make your own preps. Recipient Instructions What to do when you receive your viral vector...storage. Biosafety Guide Documentation for your institutional biosafety office and your lab, information on...

...CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur has deposited a collection of CRISPR arrays...protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going to bind...

... Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized Pool... packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature...

...two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit: raptor for mTORC1, and ...but it is activated by growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal...

...factors), and downstream (by GAPs, GTPase activating proteins, which also serve effector roles). A compatible..., & Ettema, T. J. (2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building...

...s lab. It is derived from the AAV2 capsid and contains the following mutations: Y444F, Y500F, and Y730F...s lab. It is derived from the AAV6 capsid and contains the following mutations: Y705F, Y731F and T492V...

... control AAV collection, including fluorescent proteins, spaghetti monster, and more....Browse our control AAV that express fluorescent proteins under general promoters. These AAV can be used...

...Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder (Link opens in a new window) Identifies...Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR & gRNA plasmids ...

...specific populations of brain cells The Allen Institute for Brain Science , Bosiljka Tasic Melina Fan ... Direct-expressing oNigri AAV Virus The Allen Institute for Brain Science , Tanya Daigle James M. Wilson...

...Huang, et al., 2019) for LY6A-permissive mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display...tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations...

...Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under the control ...minBG RiboL1-jGCaMP8s none Constitutive 1 Allen Institute for Brain Science , Jonathan Ting Calcium Sensor...

...elements is followed by their insertion into the p1316 plasmid that contains an upstream CMV promoter for... coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Hybridoma-derived...chain across the ER membrane. Downstream of the insert is a heavy chain constant domain (C H ) that is...

...Consortium Plasmids All SGC Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Genomics Consortium Plasmids - Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...Genomics Consortium Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...

...Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...