We narrowed to 137 results for: ica
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TypeCollection...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...
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Zinc Finger Consortium: OPEN Reagents
TypeCollection...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your... -
Mammalian RNAi Tools
TypeCollection...Molecular mechanisms and biological functions of siRNA . International Journal of Biomedical Science, 13 (2), ...search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...Scientists using these zinc finger arrays in a publication should notify [email protected] and...acknowledge NIH grant number R01 GM088040 in the publication. References Rapid 'open-source' engineering of...zinc-finger nucleases for highly efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. ... -
p53 Pathway
TypeCollection...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific... of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury MP, Bourdon JC. ... -
Lentiviral Prep Service
TypeCollection...libraries to screen for genes involved in specific biological processes. For more information, see our Guide...Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human ... Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator... -
Open Enzyme Collection
TypeCollection...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended... -
Viral Production
TypeCollection...addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources Browse...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties... -
Brain Armamentarium
TypeCollection...(yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons Jonathan Ting Viviana Gradinaru...protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan Ting.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru 163505-PHPeB... -
Ras Pathway
TypeCollection... removal of a phosphate group. Under normal physiological conditions GTPases play prominent roles in signaling... below. Color is used for clarity and does not indicate a specific relationship. The content and map for...Research Color is used for clarity and does not indicate a specific relationship. The content and map for...