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We narrowed to 13 results for: Blue fluorescent Protein

Showing: 1 - 13 of 13 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...Structure Plasmids Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(blue), 6.8(red) 2.8/4.1 7.1 hr (red...Bacterial Expression Medium-FT 401(blue), 579(red) 464(blue), 600(red) 18.4(blue), 5.8(red) 2.7/4.7 3.9 hr (red...Bacterial Expression Slow-FT 402(blue), 583(red) 465(blue), 604(red) 11.7(blue), 4.2(red) 2.6/4.6 28 hr (red... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...

  2. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection More Fluorescent...lab fluorescent protein fusion constructs and backbones. Plasmid... Plasmid Collections Fluorescent Proteins Michael...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian...empty backbone with different fluorescent tags for you to create fusion proteins with your gene of interest...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael...more information. Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty...

  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are... S, Lim WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources Teusink..., Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb 19;9...

  4. Optogenetics Plasmid Collection

    Type
    Collection
    ...Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch... activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...types of proteins can also be controlled by light, generally by utilizing a light sensitive protein from ...light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as an optical switch to control protein interactions...Plasmids Background Optogenetics plasmids encode proteins that are controlled by light. Many of these plasmids... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing... is a membrane channel that opens in response to blue light and allows cations to flow into the cell. ...

  5. Bacterial Expression Systems

    Type
    Collection
    ...Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein Interactions Förster...Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization... Split Fluorescent Proteins for Studying Protein–Protein Interactions Return to top Controlled Expression...that can be used for protein purification, protein visualization, study of protein interactions, controlled...Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled Expression...plasmids that contain fluorescent protein (FP) tags so you can express your protein of interest fused to... Additional Addgene Protein Visualization Resources Visit our Fluorescent Protein Guide to find an extensive...

  6. Antibody Production

    Type
    Collection
    ...then purified from the supernatant using Protein A or Protein G affinity columns. Formulation Buffer After...known concentration and stained with Coomassie blue. Protein band intensities are measured using ImageJ software...measured and the ratio of the antibody protein content to the total protein content of the sample is determined...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...secondary antibody. Protein expression is compared to an untransfected control and the protein size is confirmed...by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies, IPI will...these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage. Antibody...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based Glucose...Lactate Lindoblum green fluorescent biosensor for lactate Green fluorescent protein-based lactate and pyruvate...Pyruvate Pegassos green fluorescent biosensor for pyruvate Green fluorescent protein-based lactate and pyruvate...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein Common...-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector...Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further ...Another common scenario is fusing your protein to another protein, such as GFP, which allows you to...localization of your protein. Additionally, tags are commonly added to aid in protein purification. Just...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-...multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael Davidson...

  9. Antibody Guide

    Type
    Collection
    ...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

  10. Optogenetics AAV Preps

    Type
    Collection
    ...Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein Activity...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ... the protein. 49043 EMM67 49044 EMM71T Golden Gate TALEN 2.0 49401 pBlue-TAL Michal Zurovec pBlue-TAL ...vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully). This next...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...deletion from the N terminus of the wild-type TALE protein (previously shown to preserve the DNA-binding ability...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning... Golden Gate TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...amino acids) fragments for protein-protein interaction assays. Each target protein is fused to either LgBiT...Constructs Other Highlights You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies ...Luciferase Complementation assays can report on protein-protein interactions, drug-induced changes, and more... about BRET on our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns...Both firefly and Renilla luciferase have short protein half-lives, making them useful as transcriptional...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...ATP-independent, and a secreted version of the protein is available, making it ideal for a variety of ...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Michael Ristow 14987 pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA ...Ristow 14989 pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-...Vale 15402 pBlue-hFX(AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F...Ristow 15404 pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...
Showing: 1 - 13 of 13 results