We narrowed to 29 results for: Cre expression vector

... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find...or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre , other...Summary of Cre, Dre, and Flp Recognition Sites. Controlling Recombinase Expression By expressing the enzyme...interest will prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised...lox-STOP-lox shRNA constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs...Genes A and B. When Cre is absent, only Gene A is translated correctly. Cre expression excises Gene A and...double-crossover event. Toxicity Cre-lox recombination is a very useful tool, but Cre expression can be toxic in some...

...Collections Gene Vector Core Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre ... pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato... transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed...by another vector pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed...expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 Cre is coexpressed with tdTomato and ...

...pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase. Takuji Iwasato...pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase. Floris Foijer...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...165457 AAVS1-Neo-TRE-CMV-Cre-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...

... empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily...developed and characterized which allow researchers to express desired constructs in precise cell types and/or...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...

... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...

...2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing mouse. Described...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for brain...NeuN)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA...LacZ)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA...backbone)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA...addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase-2A-EGFP-KASH ...

...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells...AAV-pgk-Cre PGK Cre expression Recombinases Patrick Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre...Cre.SV40 CamKII Cre expression Recombinases James M. Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression...physically-separate mCherry expression Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases...-tagged Cre expression Recombinases James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression...simultaneous dTomato expression Recombinases Rylan Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases... Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde...

...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP...2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby... a 'Cre-on' dependent manner. 1 Don Arnold 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent...pAAV-FLEx-ER-TurboID CMV-driven, Cre-dependent Cre-dependent expression of ER-localized TurboID (with HA...-mKate2-PDE4D3-Cat EF1a-driven, Cre-dependent Cre-dependent expression of mKate2 and a truncated human...pAAV-FLEX-P301L Tau EF1a-driven, Cre-dependent Cre-dependent expression of human 2N4R microtubule-associated... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...

...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...-AAV5 pAAV-CAG-DIO-PPO-Venus AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven...AAV8 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven...AAV9 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization...

...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738... 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA minBG iCre expression iCre Ting 199775 AiP13778 - pAAV-AiE0450h-minBG-iCre...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) minBG iCre(R297T) expression iCre AIBS , Ting 199776...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) minBG iCre(R297T) expression iCre AIBS , Ting 199777...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) minBG iCre(R297T) expression iCre AIBS , Ting 214862...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) minBG iCre(R297T) expression iCre AIBS , Ting PHP.S...

...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression in striatal ..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression in striatal ...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression in striatal ...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...

...Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre...recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more information ...pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11...Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Hongkui Zeng...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato ...information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40... 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg* Esteban Engel , Alexander...

...cells expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in...EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff EYFP Cre AND...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre... Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to... A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations expressing only...

... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These....1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...

...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance...Luciferase MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044 pMIG MSCV Plasmid...Plasmid for transgene expression with GFP expression. See plasmid 12282 for the addition of WRE, a post-transcriptional...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional...pLncEXP CMV/MSV lncRNA expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in ...pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid...

...Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m...224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_IT Isocortex 224038 ...220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 ...220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 ...229920 pAAV-AiE0459m_3xC2-minBG-iCre(R297T)-BGHpA AiP14187 AiE0459m_3xC2 Cre Layer 5_ET Isocortex 224013 ...

...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are described...controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...

... Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive (non-cre-dependent) Clear... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent...floxed-mCherry-WPRE-HGHpA EF1a mCherry Cre dependent 9 Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2,...EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457 pAAV-hSyn-DIO-EGFP hSyn EGFP Cre dependent 1, 2...mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent...pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Ian Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1...

...constitutive expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral...

...Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive... Calcium Intersectional viral expression of sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive...Adenosine Express the adenosine sensor GRAB_Ado1.0 in neurons or astrocytes (Constitutive or Cre-dependent...cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr: A single-wavelength fluorescent...iSeroSnFR for bacterial or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a ...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors for imaging...