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Showing: 1 - 20 of 83 results

  1. Cre-lox system

    Type
    Collection
    ...comparison of Cre+ and Cre- cells. While Cre-lox recombination is a very useful tool, Cre expression can be ...-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (...recombinant Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc CAG Mammalian Cepko 13776 pCAG-Cre:GFP Cre-GFP fusion...pOG231 NLS-Cre CMV Mammalian Wahl 19131 pIC-Cre Cre Bacterial Rajewsky 22776 MSCV CreERT2 puro Cre-ERT2 - ...25997 LV-Cre pLKO.1 Cre and shRNA coexpression CMV Lentiviral Fuchs 26646 pCAG-Cre-IRES2-GFP Cre and GFP...34564 pMSCVpuro-Cre Cre 5'LTR Retroviral Ge 34565 pMSCVhygro-Cre Cre and HygroR coexpression Retroviral Ge...DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian Zeng 61574 Slc17a7-IRES2-Cre targeting vector Cre; Targeting...

  2. Brain Initiative Collection

    Type
    Collection
    ...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...-AAV5 pAAV-CAG-DIO-PPO-Venus AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven...AAV8 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven...AAV9 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing mouse. Described...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for brain...mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for cancer modeling Using Cas9 mice, Platt...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase...target site sequence (20bp). CRISPR-Cas9 Cre expression vectors for genome editing in the brain Using Cas9...

  4. AAV Molecular Tools

    Type
    Collection
    ...amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP...2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby...Name Expression System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent...pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118...

  5. Mammalian RNAi Tools

    Type
    Collection
    ...Trono Cre-regulated Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression ID Plasmid...Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative control vector for ...selection of empty vectors for cloning in shRNAs. Plasmids for constitutive expression as well as those ...those that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing...Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression ID Plasmid Description PI 10878...10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended for cloning new shRNAs (contains a 1.9 kb...Negative control vector containing scrambled shRNA David Sabatini 11795 pLL3.7 Expresses shRNA under the...

  6. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of Medicine Gene Vector Core (Link...Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed...by another vector pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed...promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato 72261 Expresses tdTomato from the ... promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre ...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate...GFP-tagged Cre expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases... 0.4.Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells...physically-separate mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases ...simultaneous dTomato expression Recombinases Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases...pAAV-EF1a-Flpo EF1a Flpo expression Recombinases Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases...

  8. Caltech Systemic Capsids

    Type
    Collection
    ...Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service... a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These viral vector preparations were produced...Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent ...sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent...sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent...

  9. Brain Armamentarium

    Type
    Collection
    ...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression in striatal ..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression in striatal ...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression in striatal ...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...

  10. Zebrafish Plasmid Collection

    Type
    Collection
    ...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are described.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that ...A useful “starter kit” for screening fluorescent protein fusion expression in organisms or cells in which...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...

  11. Viral Production

    Type
    Collection
    ... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These....1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...

  12. Retrovirus Plasmids

    Type
    Collection
    ...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses... 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see pMIG-w , a variant that...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...

  13. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none S. pyogenes Huang...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...cells expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in...Fon EYFP Cre AND Flp 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp 55651 pAAV-hSyn Con/Foff EYFP Cre AND NOT...Flp AND NOT Cre 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp...AND NOT Cre 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry Cre AND NOT...AND NOT Cre 137122 pAAV-Ef1a-Con/Fon-GCaMP6F Cre AND Flp 137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT...Fon/Von-EYFP Cre AND Flp AND Vcre 137165 pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre References ...

  15. Recombinases AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases...Deisseroth 55636 pAAV-EF1a-Cre EF1a none rg* Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1,...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion...105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Carmichael...Carmichael 107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* Wilson 24593 AAV-pgk-Cre PGK none rg* Aebischer 51507...51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...His-tagged bacterial expression vector (see tagged expression vectors from the SGC... Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi...destination vector for gene expression URA3 Yeast pXP518 - Yeast expression ... Gateway expression vector (see more Gateway vectors) TRP1 Yeast pXP216 - Yeast...Yeast expression vector (PGK1 promoter, 2μ) pXP316 - Yeast expression vector... Gateway expression vector (see more Gateway vectors) LEU2 Yeast pXP122 - ...- Yeast expression vector (PGK1 promoter, CEN/ARS) pXP222 - Yeast expression ...

  17. Worm Expression Resources

    Type
    Collection
    ... empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily...developed and characterized which allow researchers to express desired constructs in precise cell types and/or...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...

  18. Optogenetics Plasmid Collection

    Type
    Collection
    ...These proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate...identified to increase the spectral and kinetic properties as well as improve the expression and membrane...fluorescent protein to the opsin in order to track its expression and localization. Many other types of proteins...inhibition. For example, search for "ChR mCherry", "Cre inhibition", "Boyden 2014", or other terms. Looking...optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return to top Optical...other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return to top Do you...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Calcium Intersectional viral expression of sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive...Adenosine Express the adenosine sensor GRAB_Ado1.0 in neurons or astrocytes, (Constitutive or Cre-dependent...cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr: A single-wavelength fluorescent...iSeroSnFR for bacterial or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a ...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...

  20. Lentivirus Plasmids

    Type
    Collection
    ...Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See article...Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid...pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N...MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope...article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can...constitutive transgene expression, contains intron that gives you high expression. Trono 12254 pWPI 2nd... plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic expression of EGFP and the gene...
Showing: 1 - 20 of 83 results