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Showing: 1 - 18 of 18 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pRS416Gal TDP43 WT YFP TARDBP YFP GAL1 ALS Aaron Gitler 27448 pRS416 Gal G294A YFP TARDBP YFP GAL1 ALS Aaron...pRS416 Gal M337V YFP TARDBP YFP GAL1 ALS Aaron Gitler 27450 pRS416 Gal Q331K YFP TARDBP YFP GAL1 ALS Aaron...pRS303 Gal TDP-43 YFP TARDBP YFP GAL ALS Aaron Gitler 27470 pDONR TDP43 WT YFP TARDBP YFP ALS Aaron Gitler...Gitler 29592 426Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler 29593 416Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler...29594 416GPD-FUS-YFP FUS YFP GPD ALS Aaron Gitler 29595 416Gal-SV40NLS-FUS-YFP FUS YFP, SV40 NLS GAL1 ALS...426Gal-FUS-1-170aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29597 426Gal-FUS-1-270aa-YFP FUS YFP GAL1 ALS Aaron ...426Gal-FUS-1-373aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29599 426Gal-FUS-1-413aa-YFP FUS YFP GAL1 ALS Aaron ...

  2. Bacterial Expression Systems

    Type
    Collection
    ...Visualization and Tagging Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization...artifacts!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western blotting...Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent protein tags Various Plasmids Blue...interest in bacteria, add epitope tags to it, and, later, cleave those tags from the purified protein. Protein...recombinant protein with an N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP...recombinant protein with an N-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize the...IPTG inducible) N terminal GST tag with TEV cleavage site to remove tag Nicola Burgess Brown pGEX expression...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... NLS mCherry Connie Cepko 37341 pQC NLS YFP IX Nucleus NLS YFP Connie Cepko 158000 pCaggs-NLS-PAmCherry1...miRFP703 Vladislav Verkhusha 1816 Lamp1-YFP Lysosomes Lamp1 YFP Walther Mothes 1817 Lamp1-RFP Lysosomes...Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc...Mothes 79806 pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-...endosomes Rab11a TagBFP James Johnson 79800 pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ...C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging in Drosophila... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse...allow tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods...and throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids...design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System The Eric Mendenhall...deposited plasmids in this CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG ...Protocol 134.7 KB Mendenhall and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA ...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...C-terminal 3xHA tag for yeast expression Myc Epitope Tag pKMyc - N-terminal Myc tag for mammalian...araBAD p15TV-L - His-tagged bacterial expression vector (see tagged expression vectors from...Expression with Strep, His or optimized YFP tags for purification or localization ...promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - Easy cloning...Gateway recombination Back to Top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools... 2xFLAG tag in pCS for a variety of systems, including Xenopus HA Epitope Tag pKH3 - N... 3xHA tag for mammalian expression pHAHA - N-terminal double HA tag for mammalian...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene...Maturation Structure Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz...Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial...empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your gene... iRFP Gradia Lab Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab ...C. elegans Hamdoun Lab Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP pPD95...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...categories of biosensors or browse all the biosensors tagged in our catalog . Use the article links to find ... 2013 Nov 13;7:202. Colin Akerman Chloride (Cl-) YFP-derived sensor for measuring Cl- concentration in...in physiological range A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability...by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy. 2007;3(5):452-60. Tamotsu Yoshimori...Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy...Hoffman Voltage JEDI-2P voltage indicator for two-photon imaging Sustained deep-tissue voltage recording...Rafael Yuste Voltage Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium...

  8. Brain Armamentarium

    Type
    Collection
    ...pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting...pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting...pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting...pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-...pAAV_BiVIPe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting...pAAV_BiCHATe27_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting...pAAV_BiSSTe10_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting...

  9. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...C-terminal His tag SYFP2 Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover ... suitable for creating individual fluorescently tagged proteins to study protein-protein interactions ...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest ... EYFP connected by 8 GGSGGS repeats pET28CLY9 Peptide linker standard consisting of ECFP and EYFP connected...pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected by 1 flexible glycine- and serine-containing...pET28CLY2 Peptide linker standard consisting of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide ...

  10. Control AAV Preps

    Type
    Collection
    ...Fluorophore/Tag Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive...Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Sanes 45186 AAV-EF1a-BbChT... EGFP Constitutive 5 Haydon 104055 pAAV-CAG-eYFP CAG EYFP Constitutive 2, 5, rg*, PHP.eB Gradinaru 104061...Constitutive 5 Khakh 105622 pAAV.CamKII(1.3).eYFP.WPRE.hGH CamKII(1.3) eYFP Constitutive 1 Deisseroth 105921 pAAV-CBh-mKate2... 1, 2, 5, 8, 9, rg* Deisseroth 117382 hSyn1-eYFP hSyn eYFP Constitutive 2, 5, 8, 9, rg*, PHP.eB Gradinaru...Constitutive 9, PHP.eB Feng 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Deisseroth 28306...

  11. Brain Initiative Collection

    Type
    Collection
    ...Fluorescent reporter for serotonin (dendrite localization tag) Lin Tian 135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for...AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used ...with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for...-CAG-DIO-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the...AAV2 pAAV-CAG-eYFP An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG ...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn EGFP-tagged Cre expression Recombinases Wilson 105551 pENN....pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged Cre expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH...pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent...Fon/Von eYFP nEF EYFP, Cre, Flp and VCre-dependent Control Deisseroth 117382 hSyn1-eYFP Syn EYFP Control...Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth 28306...Dlx dTomato Control Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 112677 pOTTC1032 - pAAV EF1a... 3.0-EYFP EF1a Inhibitor, Cre-dependent Optogenetics Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn ...

  13. AAV Molecular Tools

    Type
    Collection
    ...pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a 5, rg* Heintz , Nectow , Schmidt...TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru... Flp-dependent Flp-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate...

  14. Optogenetics AAV Preps

    Type
    Collection
    ...PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV ...ET/TC)-EYFP nEF ChR2(E123T/T159C) EYFP Flp dependent 8 Deisseroth 26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA...E123T/H134R)-eYFP.WPRE.hGH CaMKII ChETA EYFP Constitutive 9 Deisseroth 135633 pAAV-S5E2-C1V1-eYFP E2 C1V1 ...Arch3.3-EYFP nEF Arch3.3 EYFP Cre dependent 8 Deisseroth 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP nEF Arch3.3...Arch3.3 EYFP Flp dependent 8 Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent...pAAV-CaMKIIa-eNpHR 3.0-EYFP CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Deisseroth 26972 pAAV-hSyn-eNpHR 3.0-EYFP Syn eNpHR...eNpHR 3.0 EYFP Constitutive 5 Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 ...

  15. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2402m-minBG-SYFP2-WPRE3-BGHpA AiP1706 AiE2402m SYFP2 Layer 5_ET Isocortex 223981 pAAV-AiE0464m-minBG-SYFP2-WPRE3...pAAV-AiE0674m-minBG-SYFP2-WPRE3-BGHp AiP12857 AiE0674m SYFP2 Layer 5_ET Isocortex 224017 pAAV-AiE0667m-minBG-SYFP2-WPRE3...Region 230551 pAAV-AiE2638m-minBG-SYFP2-WPRE3-BGHpA AiP2105 AiE2638m SYFP2 Layer 2-3_IT Isocortex 230803 ...Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2-WPRE3-BGHpA AiP1924 AiE2543m SYFP2 Layer 2-3_IT Isocortex 220727 ...Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2-WPRE3-BGHpA AiP12754 AiE0680m SYFP2 Layer 2-3_IT Isocortex 230402 ...223974 pAAV-AiE0050m_3xC2-minCMV-SYFP2-WPRE3-BGHpA AiP12211 AiE0050m_3xC2 SYFP2 Layer 4_IT Isocortex 224056...224038 pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA AiP13897 AiE0671m_3xC2 SYFP2 Layer 4_IT Isocortex 220734...

  16. Caltech Systemic Capsids

    Type
    Collection
    ...rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA minBetaGlobin SYFP2 Control Ting 163509 CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3...Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP...EF1a mCherry Control Deisseroth 117382 hSyn1-eYFP Syn EYFP Control Gradinaru 135630 pAAV-S5E2-dTom-nlsdTom...-BGHpA hSyn1 H2B-SYFP2 Control Ting 191706 AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias...: CN3044) minBG SYFP2 Control AIBS , Ting 191707 AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias...CN2237) minBG SYFP2 Control AIBS , Ting 191720 AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (...CN3038) minBG SYFP2 Control AIBS , Ting 191726 AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (...

  17. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...H134R)-eYFP.WPRE.hGH Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics...pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics...eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-5-26968P 26968-AAV5 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics...pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth AV-5-26972P 26972-AAV5 pAAV-hSyn-eNpHR 3.0-EYFP Optogenetics...floxed-eNpHR-EYFP-WPRE-pA Optogenetics Karl Deisseroth AV-9-26966P 26966-AAV9 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics...H134R)-eYFP.WPRE.hGH Optogenetics Karl Deisseroth AV-9-26968P 26968-AAV9 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics...pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth AV-9-26971P 26971-AAV9 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    .../9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [N77... Human Mouse IgG2a 114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3... Human Mouse IgG2a 114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a 114483 Anti-GluA1...) Rat Mouse IgG2a 114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin... Mouse Mouse IgG2a 177526 Anti-Synaptotagmin-7 [N275/14R] Synaptotagmin-7 Mouse Mouse IgG2a 177527 Anti-Lgi1... Human Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK...Shank3 Rat Mouse IgG2a 188223 Anti-Synaptotagmin-9 [N276A/15R] Synaptotagmin-9 Mouse Mouse IgG2a 188224 Anti-TrpV1...
Showing: 1 - 18 of 18 results