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We narrowed to 13 results for: acr.4

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Showing: 1 - 13 of 13 results

  1. CRISPR Guide

    Type
    Collection
    ...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved...Nature Biotechnology . 41 (4), 500–512. https://doi.org/10.1038/s41587-022-01527-4 PMID: 36424489 Genome-wide...More commonly, Acrs inhibit multiple species of Cas enzymes, such as AcrIIA2 and AcrIIA4, isolated from...There are rare cases where Acr can work with multiple types, such as AcrVA3.1, which inhibits both Cas12a...Similarly, some Acr proteins are specific to a particular species of CRISPR enzymes, such as AcrIIA7–10, which... protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled Acr activity ... to protect against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety of mechanisms...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...heavy diversity 4-23 (non-functional) IGHD423 IGHD4-4 immunoglobulin heavy diversity 4-4 DA4, IGHD44 IGHD5...heavy variable 4-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304...immunoglobulin heavy variable 4-39 IGHV439, VH IGHV4-4 immunoglobulin heavy variable 4-4 IGHV44, VH IGHV4-59 immunoglobulin...104A BD-4, DEFB-4, DEFB104, DEFB4, MGC118942, MGC118944, MGC118945, hBD-4 DEFB4 defensin, beta 4 DEFB-2...lambda variable 4-3 IGLV43, V5-1 IGLV4-60 immunoglobulin lambda variable 4-60 IGLV460, V5-4 IGLV4-69 immunoglobulin...hIL-3Ra IL4 interleukin 4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402 IL4R interleukin 4 receptor CD124, IL4RA ...

  3. Genetic Code Expansion

    Type
    Collection
    ...Thomas Huber 207620 pRSF-G1(4/5FTrp)RS G1(4/5FTrp)RS Methanogenic archaeon 4-Fluoro-L-Tryptophan (4FTrp...jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram 182884 AzoRS-4 tyrosyl-tRNA...4CNW)RS G1(4CNW)RS Methanogenic archaeon 4-Cyano-L-Tryptophan (4-CN-Trp) Bacterial TAG Thomas Huber 222872... Methanogenic archaeon S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine...pDule-IBBN (G2) IBBN (G2) synthetase M. jannaschii 4-(2′-bromoisobutyramido)-phenylalanine (IBBN) and structurally...pDule2-IBBN (G2) IBBN (G2) synthetase M. jannaschii 4-(2′-bromoisobutyramido)-phenylalanine (IBBN) and structurally...pRSF-G1mCNPRS G1mCNPRS Methanogenic archaeon L-3-(2-cyano-4-pyridyl)alanine (mCNP) Bacterial TAG Thomas Huber ...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...single-cell resolution. PLoS Biol. 2025 Apr 29;23(4):e3003048. Kiryl Piatkevich Calcium Fast red fluorescent...tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25. Martin Webb ATP Multicolor palette of ATP...Dynamics. Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. Edward Boyden cAMP (cyclic AMP) FRET sensors of cAMP... and with high affinity. PLoS One. 2015 Apr 14;10(4):e0122513. Kees Jalink cAMP (cyclic AMP) Fluorescent... chloride in neuronal processes. PLoS One. 2012;7(4):e35373. Susan Amara , Susan Ingram Citrate Nanosensors...Single beta Cells. Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. Dmytro Yushchenko Lactate Laconic FRET-based... Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. Gary Yellen NADH/NAD+ Peredox fluorescent...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA. (4) Each crRNA...double-stranded DNA cleavage. Nature . 533(7603):420-4. PMID: 27096365 Ma H, Naseri A, Reyes-Gutierrez P,...Using the CRISPR/Cas9 System. Science . 343(6166):80-4. PMID: 24336569 Zetsche B, Gootenberg JS, Abudayyeh...trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse...cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break...

  6. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Species Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4 [N222...Human Mouse IgG2a 140081 Anti-Neuroligin-4* [N98/7R] Neuroligin-4* Mouse Mouse IgG2a 149451 Anti-GluK5/Grik5...Rat Mouse IgG2a 177522 Anti-TARPGamma2/4/8 [N245/36R] TARPGamma2/4/8 Rat Mouse IgG2a 177523 Anti-Cav1.2 ...Rat Mouse IgG2a 188168 Anti-Neuroligin-4* [N98/47R] Neuroligin-4* Mouse Mouse IgG2a 188169 Anti-Neuroligin...Human Mouse IgG2a 220426 TARPGamma2/4/8 [N245/36R-2b] TARPGamma2/4/8 Rat Mouse IgG2a 220427 Kir2.3 K+ ...231843 TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody TARPGamma2/4/8 Rat Chimera...Human Mouse 211607 TRIP8b (exon 4) scFv [N212/3] N212/3 scFv TRIP8b (exon 4) Rat Mouse 211608 Synaptotagmin...

  7. mTOR Pathway

    Type
    Collection
    ..., Topisirovic I. Nat Rev Drug Discov. 2015 Apr;14(4):261-78. doi: 10.1038/nrd4505. PubMed PMID: 25743081...proliferation, including an abundant supply of macromolecules and energy, as well as the presence of both...

  8. Brain Initiative Collection

    Type
    Collection
    ...Fishell 83897-AAV1 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83897-AAV9 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83897-AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain...AAV9 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and ...

  9. Resolute Plasmid Collection

    Type
    Collection
    ...560-598. https://doi.org/10.1038/s44320-025-00106-4 (Link opens in a new window) PMID: 40355754 (Link ...Pütter, V., Zindel, D., Hamilton, B., Lenter, M., Santacruz, D., Viollet, C., Whitehurst, C., Johnsson, K....Klimek, C., Garcia, J., Serrano, I., Reil, D., Santacruz, D., Piotrowski, M., Noell, S., Bueschl, C., Li...

  10. Retrograde AAV viral preps

    Type
    Collection
    ... Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hDlx Activator DREADD Gordon Fishell 98747 pAAV-FLEX-EGFPL10a...pAAV-CKIIa-stGtACR2-FusionRed CamKII Inhibitor Optogenetics Ofer Yizhar 105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ...Shalem O, Zhang F. Nat Methods . 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed . Return to GeCKO...CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also... cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013). Additionally...or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes...repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids...

  12. Plan Your Experiment

    Type
    Collection
    ...2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert, L. A., Horlbeck, M. A., Adamson...(2023). Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent ...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...deltaα4) CHMP2B HA CMV ALS Franz-Ulrich Hartl 232009 pCMV CHMP2B-L4D F5D-d55-138-3xHA (deltaα2-4) CHMP2B...3: TLS.pRSET.C FUS His T7 ALS David Ron 21830 TLS 4: hTLS-CHOP.pBSKS(+) FUS T7 ALS David Ron 21831 TLS...dementia (FTD) Thomas Kukar 176924 pcDNA3.1(+)_Granulin 4+linker5 GRN Flag, Strep CMV Frontotemporal dementia...dementia (FTD) Thomas Kukar 176925 pcDNA3.1(+)_Granulin 4-no linker GRN Flag, Strep CMV Frontotemporal dementia...Alzheimer's, ALS Tatyana Shelkovnikova 237548 TDP-43 deltaCR GFP TARDBP GFP CMV Alzheimer's, ALS Tatyana Shelkovnikova...
Showing: 1 - 13 of 13 results